Visual Poetry in the Network of the International Communication. Terms, Categories, Typology

The doctoral thesis is concerned diachronically with the term "visual poetry". The focus is on its progress during the period after the Second World War in the context of the extensively advanced movement, as substantially defined in international expert discussion. The use of the term, which is supported by the arguments of many theoreticians and artists of the period, is studied in the thesis from the viewpoint of the inspirational sources and specific contacts between art and literature. The focus is on the specific positions and strategies of visual perception. Visual poetry is studied - considering the different contexts of the European avant-garde movements and through the analysis of contemporaneous theories which defined three main lines of thought - as an incoherent art stream manifesting itself in the eclectic use of artistic media. In conclusion, the thesis looks at the project of Czech author poetics typology which grew from purely Czech examples based on formal language of the visual poetry works. The thesis seeks to point out the original aspects of the internal discussion of visual poetry, and to place it in the international context

MOESM10 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 10: Table S10. Tukey HSD for mRNA 2 (Experiment 6)

MOESM6 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 6: Table S6. Tukey HSD for acetylation H3K14 at promoter 3 (Experiment 4)

MOESM8 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 8: Table S8. Tukey HSD for corticosterone analysis (Experiment 5)

Effects of ACT and AVP on AQP4 expression in astrocytes.

<p>Time course of the expression of AQP4 mRNA and protein in astrocytes at 6, 12, 24, and 48 h after treatment with AVP and combination of ACT and AVP. (A, B) The expression of AQP4 mRNA was analyzed by quantitative real-time RT-PCR and compared to the respective untreated controls. The expression, standardized by the level of GAPDH, is shown as folds of expression. (C, D) The AQP4 protein level was analyzed by Western blotting. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p

V1aR inhibitor reduces astrocyte damage and cellular AQP4 expression.

<p>(A) Hoechst 33342 and PI double-staining in astrocytes. Cells were treated with ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 for 48 h. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst stained cells. (C) The expression of AQP4 mRNA at 48 h after ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 were analyzed by quantitative real-time RT-PCR. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control, # <i>p</i> < 0.05.</p

Identification of candidate causative factors of HE with a 2-DE-based proteomic analysis.

<p>Identification of candidate causative factors of HE with a 2-DE-based proteomic analysis.</p

Alpha-1 antichymotrypsin is involved in astrocyte injury in concert with arginine-vasopressin during the development of acute hepatic encephalopathy - Fig 1

<p><b>The growth and viability of human astrocytes cultured with plasma from mini-pigs with HE and the identification of candidate causative factors for HE by a 2-DE-based proteomic analysis</b> (A) Experimental design: Mini-pig plasma was collected before the administration of α-amanitin and LPS, when encephalopathy occurred after the addition of toxins, and after treatment with extracorporeal circulation. (B) Astrocytes were cultured in medium with the collected plasma for 24 h. (C) Representative 2-DE gel images of silver-stained plasma proteins. These were based on LC-MS/MS data obtained from the NCBInr database using the MASCOT searching program. The labels 1 to 7 indicate the seven proteins that were upregulated before BAL treatment in comparison to normal plasma and which were downregulated after BAL treatment in comparison to before BAL treatment. (D) Astrocytes were incubated with various concentrations of ACT (0.01–0.5 mg/ml) for 24 h. Cell nuclei were counterstained with Hoechst 33342 with wavelengths of 350 nm excitation and 460 nm emission. Bars indicate the mean ± standard error of the mean (<i>n</i> = 5). *<i>p</i> < 0.05, in comparison to control (normal plasma).</p

Effects of ACT and AVP on astrocyte damage.

<p>(A) Astrocyte cells were co-stained with Hoechst 33342 and PI. Cells were treated with AVP (100 nM) and combination with ACT (0.5 mg/ml) and AVP for 48 h. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst stained cells. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p

ACT induces astrocyte damage.

<p>(A) Astrocyte cells were co-stained with Hoechst 33342 and propidium iodide (PI). Cells were treated with NH₄Cl (5 mM) and ACT (0.5 mg/ml) for 48 h. Final observation was conducted by fluorescence microscopy. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst-stained cells. Cells stained by PI represent dead cells, whereas Hoechst 33342 staining reveals all nuclei. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p