17 research outputs found
Why Congressional Republicans may come to regret moving so quickly with their Obamacare repeal and replacement bill.
Congressional Republicans are now moving rapidly to push through the American Health Care Act, which would repeal and replace President Obama’s signature health care reform, Obamacare. Jonathan Lewallen writes that the GOP’s haste could backfire on them. Writing legislation is a skill, he argues, and in a time of both unified government and budget cuts to Legislative Counsel offices, the chances of the party making a legislative mistake are considerably increased
Recommended from our members
You better find something to do : lawmaking and agenda setting in a centralized Congress
The U.S. Congress has significantly curtailed its lawmaking activities in recent years, and many commentators, scholars, and legislators themselves point to a decline in the institution’s output. Two trends blur this focus. First, the number of substantive (non-commemorative) laws enacted by Congress did not significantly decline until very recently. Second, that the roots of this decline have been growing for several decades, in the committee system. Data from 1981 to 2012 show that congressional committees have significantly shifted their activity towards oversight and other non-legislative policymaking at the expense of advancing legislation. Congressional committees act as Congress’s agenda setting capacity by determining what issues the institution can and will address and how it does so. Any explanation for a decline in congressional lawmaking, therefore, must begin with committees. I develop a theory of committee policymaking in this dissertation based on the limited agenda space decisionmakers face. Making policy through legislative or non-legislative means involves opportunity costs, and committees face uncertainty about whether their legislative work will bear fruit. With this theory as a guide, I test three explanations for the longitudinal shift in committee activity away from legislation. While current and former members of Congress, commentators, and other observers blame political gridlock and an expanding executive branch, I find that changes made to the legislative process itself have altered the incentives for committees to compete for agenda space and make policy through legislation. Members of both parties have centralized agenda setting responsibilities under party leaders over the last three decades, which has altered the contours and availability of legislative authority. My findings have important implications for Congress’s role in the policy process and how scholars and citizens evaluate the institution, including the importance of committee incentives and capacity for congressional agenda setting.Governmen
Protein arginine methyltransferases PRMT1, PRMT4/CARM1 and PRMT5 have distinct functions in control of osteoblast differentiation
Osteogenic differentiation of mesenchymal cells is controlled by epigenetic enzymes that regulate post-translational modifications of histones. Compared to acetyl or methyltransferases, the physiological functions of protein arginine methyltransferases (PRMTs) in osteoblast differentiation remain minimally understood. Therefore, we surveyed the expression and function of all nine mammalian PRMT members during osteoblast differentiation. RNA-seq gene expression profiling shows that Prmt1, Prmt4/Carm1 and Prmt5 represent the most prominently expressed PRMT subtypes in mouse calvarial bone and MC3T3 osteoblasts as well as human musculoskeletal tissues and mesenchymal stromal cells (MSCs). Based on effects of siRNA depletion, it appears that PRMT members have different functional effects: (i) loss of Prmt1 stimulates and (ii) loss of Prmt5 decreases calcium deposition of mouse MC3T3 osteoblasts, while (iii) loss of Carm1 is inconsequential for calcium deposition. Decreased Prmt5 suppresses expression of multiple genes involved in mineralization (e.g., Alpl, Ibsp, Phospho1) consistent with a positive role in osteogenesis. Depletion of Prmt1, Carm1 and Prmt5 has intricate but modest time-dependent effects on the expression of a panel of osteoblast differentiation and proliferation markers but does not change mRNA levels for select epigenetic regulators (e.g., Ezh1, Ezh2, Brd2 and Brd4). Treatment with the Class I PRMT inhibitor GSK715 enhances extracellular matrix mineralization of MC3T3 cells, while blocking formation of H3R17me2a but not H4R3me2a marks. In sum, Prmt1, Carm1 and Prmt5 have distinct biological roles during osteoblast differentiation, and different types histone H3 and H4 arginine methylation may contribute to the chromatin landscape during osteoblast differentiation.</p
The Global Trachoma Mapping Project: Methodology of a 34-Country Population-Based Study.
PURPOSE: To complete the baseline trachoma map worldwide by conducting population-based surveys in an estimated 1238 suspected endemic districts of 34 countries. METHODS: A series of national and sub-national projects owned, managed and staffed by ministries of health, conduct house-to-house cluster random sample surveys in evaluation units, which generally correspond to "health district" size: populations of 100,000-250,000 people. In each evaluation unit, we invite all residents aged 1 year and older from h households in each of c clusters to be examined for clinical signs of trachoma, where h is the number of households that can be seen by 1 team in 1 day, and the product h × c is calculated to facilitate recruitment of 1019 children aged 1-9 years. In addition to individual-level demographic and clinical data, household-level water, sanitation and hygiene data are entered into the purpose-built LINKS application on Android smartphones, transmitted to the Cloud, and cleaned, analyzed and ministry-of-health-approved via a secure web-based portal. The main outcome measures are the evaluation unit-level prevalence of follicular trachoma in children aged 1-9 years, prevalence of trachomatous trichiasis in adults aged 15 + years, percentage of households using safe methods for disposal of human feces, and percentage of households with proximate access to water for personal hygiene purposes. RESULTS: In the first year of fieldwork, 347 field teams commenced work in 21 projects in 7 countries. CONCLUSION: With an approach that is innovative in design and scale, we aim to complete baseline mapping of trachoma throughout the world in 2015
Recommended from our members
Incentives and competition for information in Congress
textPolicymakers need a wide array of information for multiple purposes. Acquiring information often is costly, so it is assumed that incentives must be provided to overcome these costs and stimulate information gathering. It is further assumed that increasing the number of actors engaged in acquiring information creates free-rider problems. In 2007 the U.S. House of Representatives created a select committee to address energy and environment issues, but did not give that committee legislative authority. The new committee could not compete with others for the ability to write or amend legislation, so its presence should not have changed the standing committee’s information gathering patterns. In fact, committees did alter their hearing patterns in response to the select committee’s work. Information has jurisdictional and reputational value to policymakers in addition to the incentives it can help them obtain, and policymakers will act to acquire information even without explicit incentives to do so.Governmen
Epigenetic regulators controlling osteogenic lineage commitment and bone formation
Bone formation and homeostasis are controlled by environmental factors and endocrine regulatory cues that initiate intracellular signaling pathways capable of modulating gene expression in the nucleus. Bone-related gene expression is controlled by nucleosome-based chromatin architecture that limits the accessibility of lineage-specific gene regulatory DNA sequences and sequence-specific transcription factors. From a developmental perspective, bone-specific gene expression must be suppressed during the early stages of embryogenesis to prevent the premature mineralization of skeletal elements during fetal growth in utero. Hence, bone formation is initially inhibited by gene suppressive epigenetic regulators, while other epigenetic regulators actively support osteoblast differentiation. Prominent epigenetic regulators that stimulate or attenuate osteogenesis include lysine methyl transferases (e.g., EZH2, SMYD2, SUV420H2), lysine deacetylases (e.g., HDAC1, HDAC3, HDAC4, HDAC7, SIRT1, SIRT3), arginine methyl transferases (e.g., PRMT1, PRMT4/CARM1, PRMT5), dioxygenases (e.g., TET2), bromodomain proteins (e.g., BRD2, BRD4) and chromodomain proteins (e.g., CBX1, CBX2, CBX5). This narrative review provides a broad overview of the covalent modifications of DNA and histone proteins that involve hundreds of enzymes that add, read, or delete these epigenetic modifications that are relevant for self-renewal and differentiation of mesenchymal stem cells, skeletal stem cells and osteoblasts during osteogenesis.</p