Expression Of Line-1 In Human Somatic Tissues And The Factors Correlated With Line- 1 Expression

Despite the long-held assumption that transposons are normally only expressed in the germline, recently we discovered that full length or partial transcripts of LINE1 are frequently found in the somatic cells. However, the extent of variation in LINE1 levels across different tissues and different individuals, and the genes and pathways that are co-expressed with LINE1 are unknown. Co-expressed genes may be candidate genes that are functioning in transposon silencing. Here, we report the extent of variation in L1HS expression levels across cancer tissues and healthy tissues collected for The Cancer Genome Atlas (TCGA). L1HS is overexpressed in most of the cancer types we have studied. Our results confirm earlier reports of higher L1HS expression in the esophagus and stomach tissues. We also show that mitochondrial genes are enriched among the genes whose expression is negatively correlated with L1HS expression and that PHD fingers, bromodomains and KRAB-zinc fingers (KRAB-ZFPs) are enriched among the genes positively co-expressed with L1HS. Additionally, we studied the association of L1HS transcript level with miRNA expression, and we found several candidate miRNAs that are significantly correlated with L1HS expressio

Transcriptome Analyses of Tumor-Adjacent Somatic Tissues Reveal Genes Co-Expressed with Transposable Elements

Background: Despite the long-held assumption that transposons are normally only expressed in the germ-line, recent evidence shows that transcripts of transposable element (TE) sequences are frequently found in the somatic cells. However, the extent of variation in TE transcript levels across different tissues and different individuals are unknown, and the co-expression between TEs and host gene mRNAs have not been examined. Results: Here we report the variation in TE derived transcript levels across tissues and between individuals observed in the non-tumorous tissues collected for The Cancer Genome Atlas. We found core TE co-expression modules consisting mainly of transposons, showing correlated expression across broad classes of TEs. Despite this co-expression within tissues, there are individual TE loci that exhibit tissue-specific expression patterns, when compared across tissues. The core TE modules were negatively correlated with other gene modules that consisted of immune response genes in interferon signaling. KRAB Zinc Finger Proteins (KZFPs) were over-represented gene members of the TE modules, showing positive correlation across multiple tissues. But we did not find overlap between TE-KZFP pairs that are co-expressed and TE-KZFP pairs that are bound in published ChIP-seq studies. Conclusions: We find unexpected variation in TE derived transcripts, within and across non-tumorous tissues. We describe a broad view of the RNA state for non-tumorous tissues exhibiting higher level of TE transcripts. Tissues with higher level of TE transcripts have a broad range of TEs co-expressed, with high expression of a large number of KZFPs, and lower RNA levels of immune genes

Correction to: Transcriptome Analyses of Tumor-Adjacent Somatic Tissues Reveal Genes Co-Expressed with Transposable Elements

Following publication of the original article [1], the authors reported errors in Table 2 wherein all “KZFP” in the gene names should be changed to “ZNF”

Uso de Internet y la alfabetización en eSalud con temor al COVID-19 entre estudiantes de enfermería en Filipinas

The COVID-19 outbreak situation brought into focus not only the safety but also the mental wellbeing of nursing students. At present, the level of fear of COVID-19 and associated factors among nursing students in the Philippines is not well understood. This cross-sectional online survey determined the relationship between Internet use and eHealth literacy with fear of COVID-19. One thousand three hundred and sixty-seven (n=1,367) answered an online survey using the adopted eHealth Literacy Scale (eHEALS), and Fear of COVID-19 Scale (FCV-19S) administered from May 1 to 15, 2020. Descriptive statistics, tests for differences, and correlational analysis were performed. Results indicated that the composite score of the FCV-19S was 3.65, indicating moderate to high levels of fear. Fear of COVID-19 significantly differed based on sex, year level, and location. A significant inverse relationship was found between the average daily use of the Internet and fear of COVID-19. On the other hand, no significant association was noted between eHealth literacy and fear of COVID-19. The first wave of the COVID-19 outbreak has resulted in a far-reaching impact on nursing students' psychological wellbeing. This study highlights the value of the Internet and its use during the outbreak may not always lead to higher fear related to COVID-19. Nursing schools may need to create strategies to promote regulated and responsible Internet use, address students' mental health concerns and develop interventions to respond proactively to mitigate or reduce fear among nursing students during the pandemic.El brote de COVID-19 puso de relieve no solo la seguridad sino también el bienestar mental de los estudiantes de enfermería. En la actualidad, no se comprende bien el nivel de miedo al COVID-19 y los factores asociados entre los estudiantes de enfermería en Filipinas. Esta encuesta transversal en línea determinó la relación entre el uso de Internet y la alfabetización en eSalud con el miedo al COVID-19. Mil trescientos sesenta y siete (n = 1.367) respondieron una encuesta en línea utilizando la Escala de alfabetización en salud electrónica (eHEALS) y la Escala de miedo a COVID-19 (FCV-19S) administradas del 1 al 15 de mayo de 2020. Estadísticas descriptivas, se realizaron pruebas de diferencias y análisis correlacional. Los resultados indicaron que la puntuación compuesta del FCV-19S fue de 3,65, lo que indica niveles de miedo de moderados a altos. El miedo al COVID-19 difirió significativamente según el sexo, el año y la ubicación. Se encontró una relación inversa significativa entre el uso diario promedio de Internet y el miedo al COVID-19. Por otro lado, no se observó una asociación significativa entre la alfabetización en eSalud y el miedo al COVID-19. La primera ola del brote de COVID-19 ha tenido un impacto de gran alcance en el bienestar psicológico de los estudiantes de enfermería. Este estudio destaca el valor de Internet y su uso durante el brote no siempre puede generar un mayor temor relacionado con el COVID-19. Las escuelas de enfermería pueden necesitar crear estrategias para promover el uso de Internet regulado y responsable, abordar las preocupaciones de salud mental de los estudiantes y desarrollar intervenciones para responder de manera proactiva para mitigar o reducir el miedo entre los estudiantes de enfermería durante la pandemia.Universidad Pablo de Olavid