Biochemical and Morphological Studies of Rat Submandibular Gland: II. Partial Purification of Proteins from Granule-Rich Fraction

Soluble proteins derived from a centrifuged and filtered granule-rich fraction of homogenized rat submandibular gland were analyzed by gel filtration, ion-exchange chromatography, and polyacrylamide gel electrophoresis. Both the granule-rich fraction and final supernatant fraction contained alkaline esterase activity. The major protein component, derived from granules of the convoluted tubules, was further resolved into a series of peptides ranging in molecular weight from 9,000 to 55,000 daltons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67196/2/10.1177_00220345750540053301.pd

The effect of sample drying temperature on marine particulate organic carbon composition

Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography Methods 16 (2018): 286-298, doi:10.1002/lom3.10245.Compositional changes in marine particulate organic carbon (POC) throughout the water column trace important processes that underlie the biological pump’s efficiency. While labor-intensive, particle sampling efforts offer potential to expand the empirical POC archive at different stages in the water column, provided that organic composition is sufficiently preserved between sampling and analysis. The standard procedure for preserving organic matter composition in marine samples is to immediately store particles at -80°C to -20°C until they can be freeze-dried for analysis. This report investigates the effect of warmer drying and storage temperatures on POC composition, which applies to the majority of POC samples collected in the field without intention for organic analysis. Particle samples collected off Woods Hole, MA were immediately dried at 56°C, at room temperature, or stored at -80°C until being freeze-dried. Results show that oven- and air-drying did not shift the bulk composition (i.e., carbon and nitrogen content and stable isotope composition) of POC in the samples relative to freeze-drying. Similarly, warmer drying temperatures did not affect POC thermal stability, as inferred by ramped pyrolysis/oxidation (RPO), a growing technique that uses a continuous temperature ramp to differentiate components of organic carbon by their decomposition temperature. Oven- and air-drying did depress lipid abundances relative to freeze-drying, the extent of which depended on compound size and structure. The data suggest that field samples dried at room temperatures and 56°C are appropriate for assessing bulk POC composition and thermal stability, but physical mechanisms such as molecular volatilization bias their lipid composition.This research was funded by the National Science Foundation (NSF) Graduate Research Fellowship program and the NSF Cooperative Agreement for the Operation of a National Ocean Sciences Accelerator Mass Spectrometry Facility (OCE-0753487)

Allochthonous sources and dynamic cycling of ocean dissolved organic carbon revealed by carbon isotopes

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geophysical Research Letters 44 (2017): 2407–2415, doi:10.1002/2016GL071348.We present concentration and isotopic profiles of total, size, and polarity fractionated dissolved organic carbon (DOC) from Station ALOHA (A Long-term Oligotrophic Habitat Assessment), an oligotrophic site in the North Pacific Ocean. The data show that, between the surface and 3500 m, low molecular weight (LMW) hydrophilic DOC, LMW hydrophobic DOC, and high molecular weight (HMW) DOC constitute 22–33%, 45–52%, and 23–35% of DOC, respectively. LMW hydrophilic DOC is more isotopically depleted (δ13C of −23.9‰ to −31.5‰ and Δ14C of −304‰ to −795‰; mean age of 2850 to 15000 years) than the LMW hydrophobic DOC (δ13C of −22‰ to −23‰ and Δ14C of −270‰ to −568‰; 2470 to 6680 years) and HMW DOC (δ13C of ~−21‰ and Δ14C of −24‰ to −294‰; 135–2700 years). Our analyses suggest that a large fraction of DOC may be derived from allochthonous sources such as terrestrial and hydrothermal DOC and cycle on much longer time scales of >10000 years or enter the ocean as preaged carbon.NSF Cooperative Agreement for the Operation of a National Ocean Sciences Accelerator Mass Spectrometry Facility Grant Number: OCE-0753487; Gordon and Betty Moore Foundation Grant Numbers: GBMF3298, GBMF3794; Simons Foundation Grant Number: 3291082017-09-0

Robotic Laser-Adaptive-Optics Imaging of 715 Kepler Exoplanet Candidates using Robo-AO

The Robo-AO Kepler Planetary Candidate Survey is designed to observe every Kepler planet candidate host star with laser adaptive optics imaging to search for blended nearby stars, which may be physically associated companions and/or responsible for transit false positives. In this paper we present the results from the 2012 observing season, searching for stars close to 715 representative Kepler planet candidate hosts. We find 53 companions, 44 of which are new discoveries. We detail the Robo-AO survey data reduction methods including a method of using the large ensemble of target observations as mutual point-spread-function references, along with a new automated companion-detection algorithm designed for large adaptive optics surveys. Our survey is sensitive to objects from 0.15" to 2.5" separation, with contrast ratios up to delta-m~6. We measure an overall nearby-star-probability for Kepler planet candidates of 7.4% +/- 1.0%, and calculate the effects of each detected nearby star on the Kepler-measured planetary radius. We discuss several KOIs of particular interest, including KOI-191 and KOI-1151, which are both multi-planet systems with detected stellar companions whose unusual planetary system architecture might be best explained if they are "coincident multiple" systems, with several transiting planets shared between the two stars. Finally, we detect 2.6-sigma evidence for <15d-period giant planets being 2-3 times more likely be found in wide stellar binaries than smaller close-in planets and all sizes of further-out planets.Comment: Accepted by ApJ. Minor updates & improved statistical analysis; no changes to results. 15 pages, 13 figure

Seasonal occurrence of anoxygenic photosynthesis in Tillari and Selaulim reservoirs, Western India

© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Biogeosciences 9 (2012): 2485-2495, doi:10.5194/bg-9-2485-2012.Phytoplankton and bacterial pigment compositions were determined by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) in two freshwater reservoirs (Tillari Dam and Selaulim Dam), which are located at the foothills of the Western Ghats in India. These reservoirs experience anoxia in the hypolimnion during summer. Water samples were collected from both reservoirs during anoxic periods while one of them (Tillari Reservoir) was also sampled in winter, when convective mixing results in well-oxygenated conditions throughout the water column. During the period of anoxia (summer), bacteriochlorophyll (BChl) e isomers and isorenieratene, characteristic of brown sulfur bacteria, were dominant in the anoxic (sulfidic) layer of the Tillari Reservoir under low light intensities. The winter observations showed the dominance of small cells of Chlorophyll b-containing green algae and cyanobacteria, with minor presence of fucoxanthin-containing diatoms and peridinin-containing dinoflagellates. Using total BChl e concentration observed in June, the standing stock of brown sulfur bacteria carbon in the anoxic compartment of Tillari Reservoir was estimated to be 2.27 gC m−2, which is much higher than the similar estimate for carbon derived from oxygenic photosynthesis (0.82 gC m−2. The Selaulim Reservoir also displayed similar characteristics with the presence of BChl e isomers and isorenieratene in the anoxic hypolimnion during summer. Although sulfidic conditions prevailed in the water column below the thermocline, the occurrence of photo-autotrophic bacteria was restricted only to mid-depths (maximal concentration of BChl e isomers was detected at 0.2% of the surface incident light). This shows that the vertical distribution of photo-autotrophic sulfur bacteria is primarily controlled by light penetration in the water column where the presence of H2S provides a suitable biogeochemical environment for them to flourish.Financial support for this work was provided by the Council of Scientific & Industrial Research (CSIR) and Ministry of Earth Sciences (MoES). S. Kurian acknowledges POGO-SCOR for financial support to visit WHOI. R. Roy, G. Narvenkar and A. Sarkar received fellowship support from CSIR. D. Repeta acknowledges support from US National Science Foundation Center Award EF0424599 to the Center for Microbial Oceanography: Research and Education (C-MORE)

From nonassociativity to solutions of the KP hierarchy

A recently observed relation between 'weakly nonassociative' algebras A (for which the associator (A,A^2,A) vanishes) and the KP hierarchy (with dependent variable in the middle nucleus A' of A) is recalled. For any such algebra there is a nonassociative hierarchy of ODEs, the solutions of which determine solutions of the KP hierarchy. In a special case, and with A' a matrix algebra, this becomes a matrix Riccati hierarchy which is easily solved. The matrix solution then leads to solutions of the scalar KP hierarchy. We discuss some classes of solutions obtained in this way.Comment: 7 pages, 4 figures, International Colloquium 'Integrable Systems and Quantum Symmetries', Prague, 15-17 June 200