2014 REU Poster: Expression and Characterization of WT BMUL4434 from Burkholderia multivorans

Poster presentation at REU Summer's End Research Symposium, 2014, by REU participant Santina Johnson, Spring Hill College - Sean Elliott group, Kimberly Rizzolo lab mentorBacterial cytochrome c peroxidases (bCcPs) are di-heme enzymes that protect the cell from peroxide by reducing it to water. Alternatively, MauG is a di-heme enzyme that is similar in structure to bCcPs, yet is a poor peroxidase. Instead, MauG catalyzes the formation of tryptophan tryptophyl quinone (TTQ) the catalytic cofactor required for MADH. Previous work in the Elliott lab used bioinformatics to investigate how sequence divergence within peroxidase superfamily reflects functionality. The bioinformatics analysis led to the discovery of unreported di-heme enzymes found in all strains of Burkholderia. BMUL4434 from Burkholderia multivorans was gene synthesized and codon optimized for expression in E. coli. We expressed and purified WT BMUL4434 and obtained initial optical characterization to understand where it falls in the peroxidase superfamily.NSF-RE

IBM Cloud Strategic Audit

An examination of IBM Cloud\u27s strategy and history and a recommendation for what to do moving forward

Impact testing machine Patent

Impact testing machine for imparting large impact forces on high velocity package

Complexity models in design

Complexity is a widely used term; it has many formal and informal meanings. Several formal models of complexity can be applied to designs and design processes. The aim of the paper is to examine the relation between complexity and design. This argument runs in two ways. First designing provides insights into how to respond to complex systems – how to manage, plan and control them. Second, the overwhelming complexity of many design projects lead us to examine how better understanding of complexity science can lead to improved designs and processes. This is the focus of this paper. We start with an outline of some observations on where complexity arises in design, followed by a brief discussion of the development of scientific and formal conceptions of complexity. We indicate how these can help in understanding design processes and improving designs

Coupled Mode Theory of Electron‐Beam Parametric Amplification

A theory of parametric amplification in a filamentary electron beam by transverse fields is developed in coupled mode form. Space charge effects are neglected. In addition to beam modes at the signal frequency, beam modes at frequencies ωn=ω+ω_p, n=0, ±1, ±2…, where ω_p is the pump frequency, are coupled together. A discussion of the general form of the equations is given and reveals the circumstances under which exponential gain or periodic energy transfer between various modes can occur. When applied to quadrupole electric pump fields, a description of the quadrupole amplifiers of Adler, Wade, and Gordon is obtained. This theory is then used to evaluate the noise contribution from synchronous beam modes and higher cyclotron idler modes. Coupling by axially symmetric electric fields and by axially symmetric magnetic fields is discussed, and other amplification schemes suggested. The theory of coupling by axially symmetric fields can also be used to study lens effects on noise in the gun region

Computer program for high pressure real gas effects

Computer program obtains the real-gas isentropic flow functions and thermodynamic properties of gases for which the equation of state is known. The program uses FORTRAN 4 subroutines which were designed for calculations of nitrogen and helium. These subroutines are easily modified for calculations of other gases