1,525 research outputs found

    Doppler radar with multiphase modulation of transmitted and reflected signal

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    A microwave radar signal is generated and split by a circulator. A phase shifter introduces a series of phase shifts into a first part of the split signal which is then transmitted by antenna. A like number of phase shifts is introduced by the phase shifter into the return signal from the target. The circulator delivers the phase shifted return signal and the leakage signal from the circulator to a mixer which generates an IF signal output at the Doppler frequency. The IF signal is amplified, filtered, counted per unit of time, and the result displayed to provide indications of target sense and range rate. An oscillator controls rate of phase shift in the transmitted and received radar signals and provides a time base for the counter. The phase shift magnitude increases may be continuous and linear or discrete functions of time

    Book Review: A Provisional Gazeteer of Florida Place-Names of Indian Derivation

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    Review of A Provisional Gazeteer of Florida Place-Names of Indian Derivation, Either Obsolescent or Retained, Together with Others of Recent Application. By J. Clarence Simpson. Edited by Mark F. Boyd. (Tallahassee, Florida Geological Survey, Special Publication No. 1, 1956. X+ 158 pp. Maps.

    The Addison Blockhouse

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    The so-called Addison blockhouse, situated on the old Addison grant near the Tomoka River in coastal Volusia County, has long been an enigma. The alternatives of dating suggested by persons interested in it have ranged from the sixteenth to the nineteenth century, and its builders have variously been claimed to have been Spaniards, Englishmen, or Americans. In order to lay plans for a valid interpretation of the structure to the public, the Florida Park Service undertook a study to determine its origin and history

    Alien Registration- Griffin, John W. (Mars Hill, Aroostook County)

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    https://digitalmaine.com/alien_docs/33913/thumbnail.jp

    Methods for the Detection, Study, and Dynamic Profiling of O-GlcNAc Glycosylation

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    The addition of O-linked β-N-acetylglucosamine (O-GlcNAc) to serine/threonine residues of proteins is a ubiquitous posttranslational modification found in all multicellular organisms. Like phosphorylation, O-GlcNAc glycosylation (O-GlcNAcylation) is inducible and regulates a myriad of physiological and pathological processes. However, understanding the diverse functions of O-GlcNAcylation is often challenging due to the difficulty of detecting and quantifying the modification. Thus, robust methods to study O-GlcNAcylation are essential to elucidate its key roles in the regulation of individual proteins, complex cellular processes, and disease. In this chapter, we describe a set of chemoenzymatic labeling methods to (1) detect O-GlcNAcylation on proteins of interest, (2) monitor changes in both the total levels of O-GlcNAcylation and its stoichiometry on proteins of interest, and (3) enable mapping of O-GlcNAc to specific serine/threonine residues within proteins to facilitate functional studies. First, we outline a procedure for the expression and purification of a multiuse mutant galactosyltransferase enzyme (Y289L GalT). We then describe the use of Y289L GalT to modify O-GlcNAc residues with a functional handle, N-azidoacetylgalactosamine (GalNAz). Finally, we discuss several applications of the copper-catalyzed azide-alkyne cycloaddition “click” reaction to attach various alkyne-containing chemical probes to GalNAz and demonstrate how this functionalization of O-GlcNAc-modified proteins can be used to realize (1)–(3) above. Overall, these methods, which utilize commercially available reagents and standard protein analytical tools, will serve to advance our understanding of the diverse and important functions of O-GlcNAcylation

    Panel Discussion: Foreign Governmental Control of Multinational Corporations Marketing in the United States

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    This panel discussion focuses on debating the regulation of companies operating in more than one country. The primary emphasis is placed on oil companies

    Final Report for Intravenous Fluid Generation (IVGEN) Spaceflight Experiment

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    NASA designed and operated the Intravenous Fluid Generation (IVGEN) experiment onboard the International Space Station (ISS), Increment 23/24, during May 2010. This hardware was a demonstration experiment to generate intravenous (IV) fluid from ISS Water Processing Assembly (WPA) potable water using a water purification technique and pharmaceutical mixing system. The IVGEN experiment utilizes a deionizing resin bed to remove contaminants from feedstock water to a purity level that meets the standards of the United States Pharmacopeia (USP), the governing body for pharmaceuticals in the United States. The water was then introduced into an IV bag where the fluid was mixed with USP-grade crystalline salt to produce USP normal saline (NS). Inline conductivity sensors quantified the feedstock water quality, output water purity, and NS mixing uniformity. Six 1.5-L bags of purified water were produced. Two of these bags were mixed with sodium chloride to make 0.9 percent NS solution. These two bags were returned to Earth to test for compliance with USP requirements. On-orbit results indicated that all of the experimental success criteria were met with the exception of the salt concentration. Problems with a large air bubble in the first bag of purified water resulted in a slightly concentrated saline solution of 117 percent of the target value of 0.9 g/L. The second bag had an inadequate amount of salt premeasured into the mixing bag resulting in a slightly deficient salt concentration of 93.8 percent of the target value. The USP permits a range from 95 to 105 percent of the target value. The testing plans for improvements for an operational system are also presented
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