Delta Directions Consortium (DDC): Summary of Collaborative Pathways

The Delta Directions Consortium is an interdisciplinary network of individuals, academic institutions, non-profit organizations, and foundations that work together to create positive social change in the multi-state Mississippi Delta Region. Goals include improving public health and promoting socioeconomic development. The Consortium is not an independent non-profit organization but, rather, an alliance of partners committed to collaborative and innovative problem-solving. This document provides a summary of pathways for partners in the Delta Directions Consortium, with emphasis on substantive topics and projects. It should be read as a living document to frame ideas and approaches that will be adapted in response to the needs and interests of core partners and diverse stakeholders

Biology-specific vocabulary: students’ understanding and lecturers’ expectations of student understanding

The current A-level biology curriculum includes a broad coverage of all the biosciences which demands knowledge of a wide range of biological vocabulary. Students (n = 184) from two UK universities were presented with a list of vocabulary, associated with a ‘Revise Biology’ text which highlighted key terms that students should know. Lecturers (n = 26) were asked which of these terms they expected students to know, or be aware of. Findings revealed that students’ claimed knowledge of vocabulary exceeded lecturer expectations. In addition, there were a number of terms which students did not understand and lecturers did not expect them to know, which could be removed from A-level biology courses. This is discussed in relation to whether A-level curricula need to be so content heavy and whether lecturers would benefit from knowing more about their students’ knowledge of discipline-specific terms

Biology-specific vocabulary: students’ understanding and lecturers’ expectations of student understanding

The current A-level biology curriculum includes a broad coverage of all the biosciences which demands knowledge of a wide range of biological vocabulary. Students (n = 184) from two UK universities were presented with a list of vocabulary, associated with a ‘Revise Biology’ text which highlighted key terms that students should know. Lecturers (n = 26) were asked which of these terms they expected students to know, or be aware of. Findings revealed that students’ claimed knowledge of vocabulary exceeded lecturer expectations. In addition, there were a number of terms which students did not understand and lecturers did not expect them to know, which could be removed from A-level biology courses. This is discussed in relation to whether A-level curricula need to be so content heavy and whether lecturers would benefit from knowing more about their students’ knowledge of discipline-specific terms

CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations

CBS domains are defined as sequence motifs that occur in several different proteins in all kingdoms of life. Although thought to be regulatory, their exact functions have been unknown. However, their importance was underlined by findings that mutations in conserved residues within them cause a variety of human hereditary diseases, including (with the gene mutated in parentheses): Wolff-Parkinson-White syndrome (γ2 subunit of AMP-activated protein kinase); retinitis pigmentosa (IMP dehydrogenase-1); congenital myotonia, idiopathic generalized epilepsy, hypercalciuric nephrolithiasis, and classic Bartter syndrome (CLC chloride channel family members); and homocystinuria (cystathionine β-synthase). AMP-activated protein kinase is a sensor of cellular energy status that is activated by AMP and inhibited by ATP, but the location of the regulatory nucleotide-binding sites (which are prime targets for drugs to treat obesity and diabetes) was not characterized. We now show that tandem pairs of CBS domains from AMP-activated protein kinase, IMP dehydrogenase-2, the chloride channel CLC2, and cystathionine β-synthase bind AMP, ATP, or S-adenosyl methionine,while mutations that cause hereditary diseases impair this binding. This shows that tandem pairs of CBS domains act, in most cases, as sensors of cellular energy status and, as such, represent a newly identified class of binding domain for adenosine derivatives

Ca 2+/calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation

Ca2+/calmodulin-dependent protein kinase kinase β (CaMKKβ) is a serine/threonine-directed kinase that is activated following increases in intracellular Ca2+. CaMKKβ activates Ca2+/calmodulin-dependent protein kinase I, Ca2+/calmodulin-dependent protein kinase IV, and the AMP-dependent protein kinase in a number of physiological pathways, including learning and memory formation, neuronal differentiation, and regulation of energy balance. Here, we report the novel regulation of CaMKKβ activity by multisite phosphorylation. We identify three phosphorylation sites in the N terminus of CaMKKβ, which regulate its Ca2+/calmodulin-independent autonomous activity. We then identify the kinases responsible for these phosphorylations as cyclin-dependent kinase 5 (CDK5) and glycogen synthase kinase 3 (GSK3). In addition to regulation of autonomous activity, we find that phosphorylation of CaMKKβ regulates its half-life. We find that cellular levels of CaMKKβ correlate with CDK5 activity and are regulated developmentally in neurons. Finally, we demonstrate that appropriate phosphorylation of CaMKKβ is critical for its role in neurite development. These results reveal a novel regulatory mechanism for CaMKKβ-dependent signaling cascades

A new method for imaging nuclear threats using cosmic ray muons

Muon tomography is a technique that uses cosmic ray muons to generate three dimensional images of volumes using information contained in the Coulomb scattering of the muons. Advantages of this technique are the ability of cosmic rays to penetrate significant overburden and the absence of any additional dose delivered to subjects under study above the natural cosmic ray flux. Disadvantages include the relatively long exposure times and poor position resolution and complex algorithms needed for reconstruction. Here we demonstrate a new method for obtaining improved position resolution and statistical precision for objects with spherical symmetry

The M-sigma and M-L Relations in Galactic Bulges and Determinations of their Intrinsic Scatter

We derive improved versions of the relations between supermassive black hole mass (M_BH) and host-galaxy bulge velocity dispersion (sigma) and luminosity (L) (the M-sigma and M-L relations), based on 49 M_BH measurements and 19 upper limits. Particular attention is paid to recovery of the intrinsic scatter (epsilon_0) in both relations. We find log(M_BH / M_sun) = alpha + beta * log(sigma / 200 km/s) with (alpha, beta, epsilon_0) = (8.12 +/- 0.08, 4.24 +/- 0.41, 0.44 +/- 0.06) for all galaxies and (alpha, beta, epsilon_0) = (8.23 +/- 0.08, 3.96 +/- 0.42, 0.31 +/- 0.06) for ellipticals. The results for ellipticals are consistent with previous studies, but the intrinsic scatter recovered for spirals is significantly larger. The scatter inferred reinforces the need for its consideration when calculating local black hole mass function based on the M-sigma relation, and further implies that there may be substantial selection bias in studies of the evolution of the M-sigma relation. We estimate the M-L relationship as log(M_BH / M_sun) = alpha + beta * log(L_V / 10^11 L_sun,V) of (alpha, beta, epsilon_0) = (8.95 +/- 0.11, 1.11 +/- 0.18, 0.38 +/- 0.09); using only early-type galaxies. These results appear to be insensitive to a wide range of assumptions about the measurement errors and the distribution of intrinsic scatter. We show that culling the sample according to the resolution of the black hole's sphere of influence biases the relations to larger mean masses, larger slopes, and incorrect intrinsic residuals.Comment: 27 pages, 18 figures, 7 tables, ApJ accepte

The Black Hole Mass and Extreme Orbital Structure in NGC1399

The largest galaxies, and in particular central galaxies in clusters, offer unique insight into understanding the mechanism for the growth of nuclear black holes. We present Hubble Space Telescope kinematics for NGC1399, the central galaxy in Fornax. We find the best-fit model contains a black hole of 5.1 +-0.7 x 10^8 Msun (at a distance of 21.1 Mpc), a factor of over 2 below the correlation of black hole mass and velocity dispersion. We also find a dramatic signature for central tangential anisotropy. The velocity profiles on adjacent sides 0.5" away from the nucleus show strong bimodality, and the central spectrum shows a large drop in the dispersion. Both of these observations point to an orbital distribution that is tangentially biased. The best-fit orbital model suggests a ratio of the tangential to radial internal velocity dispersions of three. This ratio is the largest seen in any galaxy to date and will provide an important measure for the mode by which the central black hole has grown.Comment: 9 pages, accepted for publication in the Astrophysical Journa