Letter, John M. Patton Jr. to Jack, August 27, 1859

This letter, dated August 27, 1859, is presumed to be written from John M. Patton Jr.to an unidentified man named Jack. The letter discusses several legal matters and offers his services should Jack need them.https://scholarsjunction.msstate.edu/fvw-manuscripts-clarke/1001/thumbnail.jp

Decision Support in Healthcare Supply Chain Management and Pharmaceutical Inventory Control

This exploratory research examines the pharmaceutical inventory control practices of a local area hospital and demonstrates the utility of two alternative quantitative approaches. Pharmaceuticals represent a large cost factor for most hospitals due to the significant costs of these products and the storage and control requirements. The hospital stores drugs in the Central Pharmacy and at various Care Units (CUs) throughout the hospital and employs advanced technology to manage inventory and automate the ordering processes at these local storage units. This research examines current hospital policies, employs quantitative methods to improve upon these practices, and provides a decision support tool that allows managers to quickly understand operational, tactical, and strategic implications of changes in the formulary (pharmaceutical product mix available in hospital). This decision support tool affords managers the opportunity to improve management practices and the capacity to support negotiations with stakeholders on issues related to the formulary

Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate

A recently solved structure of the aquareovirus virion (Zhang, X; Jin, L.; Fang, Q; Hui, W.H.; Zhou Z.H. 3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry. Cell 2010, 141, 472-482 [1]) provides new insights into the order of entry events, as well as confirming and refining several aspects of the entry mechanism, for aquareovirus and the related orthoreovirus. In particular, the structure provides evidence of a defined order for the progressive proteolytic cleavages of myristoylated penetration protein VP5 that prime the virion for membrane penetration. These observations reinforce the concept that, much like enveloped viruses, nonenveloped virions often undergo priming events that lead to a meta-stable state, preparing the virus for membrane penetration under the appropriate circumstances. In addition, this and other recent studies highlight the increasing power of electron cryomicroscopy to analyze large, geometrically regular structures, such as icosahedral viruses, at atomic resolution

Mutational analysis of residues involved in nucleotide and divalent cation stabilization in the rotavirus RNA-dependent RNA polymerase catalytic pocket

AbstractThe rotavirus RNA-dependent RNA polymerase (RdRp), VP1, contains canonical RdRp motifs and a priming loop that is hypothesized to undergo conformational rearrangements during RNA synthesis. In the absence of viral core shell protein VP2, VP1 fails to interact stably with divalent cations or nucleotides and has a retracted priming loop. To identify residues of potential import to nucleotide and divalent cation stabilization, we aligned VP1 of divergent rotaviruses and the structural homolog reovirus λ3. VP1 mutants were engineered and characterized for RNA synthetic capacity in vitro. Conserved aspartic acids in RdRp motifs A and C and arginines in motif F that likely stabilize divalent cations and nucleotides were required for efficient RNA synthesis. Mutation of individual priming loop residues diminished or enhanced RNA synthesis efficiency without obviating the need for VP2, which suggests that this structure serves as a dynamic regulatory element that links RdRp activity to particle assembly

Maternal structure of Iberian honey bees inferred from whole mitochondrial genomes

The maternally inherited mitochondrial DNA has been the marker of choice for assessing Iberian honey bee variation, particularly the intergenic tRNA leu -cox2 region. The data generated by massive sampling of this region confirmed early findings of coexistence of African (A) and western European (M) lineages, forming a southwestern–northeastern cline, and revealed unparalleled levels of haplotype diversity and complexity. Accordingly, it has been suggested that Iberia served as a glacial refuge, and as a place of secondary contact between European and African lineages. While we have learned a great deal with this region, due to its high levels of variation and repetitive structure, there are evolutionary questions that an only be properly addressed using other mitochondrial regions. In this study, we used NGS technology to sequence the mitogenomes of 92 individuals and analyzed the data using two phylogenetic methods.We are deeply grateful to numerous people that collaborated in this study. Beekeepers from Spain and Portugal helped obtaining Iberian samples. Antonio Pajuelo provided the contacts of Spanish beekeepers. Margarida Neto, Andreia Brandão and Irene Muñoz collaborated in the sampling. Pilar de la Rúa, Wahida Loucif, Per Kryger, Bjorn Dahle, Lionel Garnery, Raffaele Dall’ Olio, and Romée van der Zee provided the reference samples. Phillip San Miguel and Paul Parker sequenced the whole genomes and Rick Westerman performed the mapping (Purdue University). Dora Henriques and Julio Chávez-Galarza are supported by Fundação para a Ciência e Tecnologia through the scholarships SFRH/BD/84195/2012 and SFRH/BD/68682/2010, respectively. This research was funded by Fundação para a Ciência e Tecnologia and COMPETE/QREN/EU through the project PTDC/BIA-BEC/099640/2008.info:eu-repo/semantics/publishedVersio

Functionalized paramagnetic nanoparticles for waste water treatment

An approach to the design, development and implementation of a new separation technology for use in the decontamination of radioactive waste streams is reported here. Calixarene-crown-6 derivatives with terminal carboxyl groups were synthesised and attached to nano-sized magnetoferritin molecules and their ability to sequester radioactive caesium(i) ions from aqueous solution was demonstrated. © 2010 The Royal Society of Chemistry

Temporal pattern of africanization in a feral honeybee population from Texas inferred from mitochondrial DNA

The invasion of Africanized honeybees (Apis mellifera L.) in the Americas provides a window of opportunity to study the dynamics of secondary contact of subspecies of bees that evolved in allopatry in ecologically distinctive habitats of the Old World. We report here the results of an 11-year mitochondrial DNA survey of a feral honeybee population from southern United States (Texas). The mitochondrial haplotype (mitotype) frequencies changed radically during the 11-year study period. Prior to immigration of Africanized honeybees, the resident population was essentially of eastern and western European maternal ancestry. Three years after detection of the first Africanized swarm there was a mitotype turnover in the population from predominantly eastern European to predominantly A. m. scutellata (ancestor of Africanized honeybees). This remarkable change in the mitotype composition coincided with arrival of the parasitic mite Varroa destructor, which was likely responsible for severe losses experienced by colonies of European ancestry. From 1997 onward the population stabilized with most colonies of A. m. scutellata maternal origin.PRODEP II - Medida 5/Acção 5.

Prospectus, February 12, 1971

S.A.S.L.A. IS STARTED FOR STUDENT BENEFIT: ILLIACS TO TAKE ACTION; Veterans Association Co-Sponsors Boy Scouts; Problems Of Our Times: This Business Of Space; Letters To Editor; An Editorial; Bull Page: Coffee House, New Club, Family Night, SASLA, W. I. U. Transfer, SWAMP, Fail Safe, Vets Meeting, Hotline, Gemini House, Wit N\u27 Wisdom, No Paper; Champion Gymnast Teaches At Parkland; Parkland Wins Triangular; Parkland Defeats Lincoln Land; District Playoffs Announced; Mad Dogs Win Green Division; Swim Meet Belley Flops; Intramural Playoffs; Indoor Track Schedule; Intramural Standings; Track Team Shows Strengthhttps://spark.parkland.edu/prospectus_1971/1012/thumbnail.jp

Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay

Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all known introduced subspecies in the United States, and 451 colonies from non-Africanized areas of the southern United States were screened to validate a rapid PCR-based assay for identiÞcation of Africanized honey bee mtDNA. This PCR-based assay requires a single enzyme digestion (BglII) of a single PCR-ampliÞed segment of the cytochrome b gene. The BglII polymorphism discriminates the mitochondrial haplotype (mitotype) of Apis mellifera scutellata L. (ancestor of Africanized bees) from that of A. m. mellifera, A. m. caucasia, A. m. ligustica, A. m. carnica, A. m. lamarcki, A. m. cypria, A. m. syriaca, and some A. m. iberiensis, but not from that of A. m. intermissa and some A. m. iberiensis. Nonetheless, given the very low frequency ( 1%) of African non-A. m. scutellata mitotype present before arrival of Africanized bees in the United States, cytochrome b/BglII assay can be used to identify maternally Africanized bees with a high degree of reliability

Mechanism for Coordinated RNA Packaging and Genome Replication by Rotavirus Polymerase VP1

Rotavirus RNA-dependent RNA polymerase, VP1, catalyzes RNA synthesis within a subviral particle. This activity depends on core shell protein VP2. A conserved sequence at the 3′ end of plusstrand RNA templates is important for polymerase association and genome replication. We have determined the structure of VP1 at 2.9 Å resolution, as apoenzyme and in complex with RNA. The cage-like enzyme is similar to reovirus λ3, with four tunnels leading to or from a central, catalytic cavity. A distinguishing characteristic of VP1 is specific recognition, by conserved features of the template-entry channel, of four bases, UGUG, in the conserved 3′ sequence. Well-defined interactions with these bases position the RNA so that its 3′ end overshoots the initiating register, producing a stable but catalytically inactive complex. We propose that specific 3′ end recognition selects rotavirus RNA for packaging and that VP2 activates the auto-inhibited VP1/RNA complex to coordinate packaging and genome replication.Molecular and Cellular Biolog