485 research outputs found
Digging the population of compact binary mergers out of the noise
Coalescing compact binaries emitting gravitational wave (GW) signals, as recently detected by
the Advanced LIGO-Virgo network, constitute a population over the multi-dimensional space
of component masses and spins, redshift, and other parameters. Characterizing this population
is a major goal of GWobservations and may be approached via parametric models.We demonstrate
hierarchical inference for such models with a method that accounts for uncertainties in
each binary mergerâs individual parameters, for mass-dependent selection effects, and also
for the presence of a second population of candidate events caused by detector noise. Thus,
the method is robust to potential biases from a contaminated sample and allows us to extract
information from events that have a relatively small probability of astrophysical origin
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines
Neuroblastoma cell lines are an important and cost-effective model used to study oncogenic drivers of the disease. While many of these cell lines have been previously characterized with SNP, methylation, and/or mRNA expression microarrays, there has not been an effort to comprehensively sequence these cell lines. Here, we present raw whole transcriptome data generated by RNA sequencing of 39 commonly-used neuroblastoma cell lines. These data can be used to perform differential expression analysis based on a genetic aberration or phenotype in neuroblastoma (e.g., MYCN ampliïŹcation status, ALK mutation status, chromosome arm 1p, 11q and/or 17q status, sensitivity to pharmacologic perturbation). Additionally, we designed this experiment to enable structural variant and/or long-noncoding RNA analysis across these cell lines. Finally, as more DNase/ATAC and histone/transcription factor ChIP sequencing is performed in these cell lines, our RNA-Seq data will be an important complement to inform transcriptional targets as well as regulatory (enhancer or repressor) elements in neuroblastoma
A polymerase chain reaction/ligase detection reaction fluorescent microsphere assay to determine Plasmodium falciparum MSP-119 haplotypes
The merozoite surface protein-1 (MSP-1) is a blood stage antigen currently being tested as a vaccine against Plasmodium falciparum malaria. Determining the MSP-1(19) haplotype(s) present during infection is essential for assessments of MSP-1 vaccine efficacy and studies of protective immunity in human populations. The C-terminal fragment (MSP-1(19)) has four predominant haplotypes based on point mutations resulting in non-synonymous amino acid changes: E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type). Current techniques using direct DNA sequencing are laborious and expensive. We present an MSP-1(19) allele-specific polymerase chain reaction (PCR)/ligase detection reaction-fluorescent microsphere assay (LDR-FMA) that allows simultaneous detection of the four predominant MSP-1(19) haplotypes with a sensitivity and specificity comparable with other molecular methods and a semi-quantitative determination of haplotype contribution in mixed infections. Application of this method is an inexpensive, accurate, and high-throughput alternative to distinguish the predominant MSP-1(19) haplotypes in epidemiologic studies
Microreactor cells for high throughput x-ray absorption spectroscopy
High-throughput experimentation has been applied to X-ray Absorption spectroscopy as a novel route for increasing research productivity in the catalysis community. Suitable instrumentation has been developed for the rapid determination of the local structure in the metal component of precursors for supported catalysts. An automated analytical workflow was implemented that is much faster than traditional individual spectrum analysis. It allows the generation of structural data in quasi-real time. We describe initial results obtained from the automated high throughput (HT) data reduction and analysis of a sample library implemented through the 96 well-plate industrial standard. The results show that a fully automated HT-XAS technology based on existing industry standards is feasible and useful for the rapid elucidation of geometric and electronic structure of materials
An Absolute Flux Density Measurement of the Supernova Remnant Casseopia A at 32 GHz
We report 32 GHz absolute flux density measurements of the supernova remnant
Cas A, with an accuracy of 2.5%. The measurements were made with the 1.5-meter
telescope at the Owens Valley Radio Observatory. The antenna gain had been
measured by NIST in May 1990 to be .
Our observations of Cas A in May 1998 yield . We also report absolute flux density measurements of 3C48, 3C147, 3C286,
Jupiter, Saturn and Mars.Comment: 30 pages, 4 figures; accepted for publication by AJ. Revised
systematic error budget, corrected typos, and added reference
The Radio Variability of the Gravitational Lens PMN J1838-3427
We present the results of a radio variability study of the gravitational lens
PMN J1838-3427. Our motivation was to determine the Hubble constant by
measuring the time delay between variations of the two quasar images. We
monitored the system for 4 months (approximately 5 times longer than the
expected delay) using the Australia Telescope Compact Array at 9 GHz. Although
both images were variable on a time scale of a few days, no correlated
intrinsic variability could be identified, and therefore no time delay could be
measured. Notably, the fractional variation of the fainter image (8%) was
greater than that of the brighter image (4%), whereas lensed images of a point
source would have the same fractional variation. This effect can be explained,
at least in part, as the refractive scintillation of both images due to the
turbulent interstellar medium of the Galaxy.Comment: To appear in AJ (8 pages, including 4 figures
High Throughput In Situ XAFS Screening of Catalysts
We outline and demonstrate the feasibility of high-throughput (HT) in situ XAFS for synchrotron radiation studies. An XAS data acquisition and control system for the analysis of dynamic materials libraries under control of temperature and gaseous environments has been developed. The system is compatible with the 96-well industry standard and coupled to multi-stream quadrupole mass spectrometry (QMS) analysis of reactor effluents. An automated analytical workflow generates data quickly compared to traditional individual spectrum acquisition and analyses them in quasi-real time using an HT data analysis tool based on IFFEFIT. The system was used for the automated characterization of a library of 91 catalyst precursors containing ternary combinations of Cu, Pt, and Au on ?-Al2O3, and for the in situ characterization of Au catalysts supported on Al2O3 and TiO2
ALMA High-frequency Long Baseline Campaign in 2017:Band-to-band Phase Referencing in Submillimeter Waves
In 2017, an Atacama Large Millimeter/submillimeter Array (ALMA)
high-frequency long baseline campaign was organized to test image capabilities
with baselines up to 16 km at submillimeter (submm) wavelengths. We
investigated image qualities using ALMA receiver Bands 7, 8, 9, and 10 (285-875
GHz) by adopting band-to-band (B2B) phase referencing in which a phase
calibrator is tracked at a lower frequency. For B2B phase referencing, it is
expected that a closer phase calibrator to a target can be used, comparing to
standard in-band phase referencing. In the first step, it is ensured that an
instrumental phase offset difference between low- and high-frequency Bands can
be removed using a differential gain calibration in which a phase calibrator is
certainly detected while frequency switching. In the next step, comparative
experiments are arranged to investigate the image quality between B2B and
in-band phase referencing with phase calibrators at various separation angles.
In the final step, we conducted long baseline imaging tests for a quasar at 289
GHz in Band 7 and 405 GHz in Band 8 and complex structure sources of HL Tau and
VY CMa at ~670 GHz in Band 9. The B2B phase referencing was successfully
applied, allowing us to achieve an angular resolution of 14x11 and 10x8 mas for
HL Tau and VY CMa, respectively. There is a high probability of finding a
low-frequency calibrator within 5.4 deg in B2B phase referencing, bright enough
to use an 8 s scan length combined with a 7.5 GHz bandwidth.Comment: 61 pages, 17 figures, 8 table
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