Time-resolved gas temperatures in the oscillating turbulent flow of a pulse combustor tail pipe

The cyclic behavior of the gas temperature in the oscillating turbulent flow in a pulse combustor tail pipe was studied using two-line atomic fluorescence. In this flow, the oscillations result from an acoustic resonance, and have amplitudes of up to 5 times the mean velocity. Oscillation frequencies were varied from 67 to 101 Hz. Spatially resolved temperature measurements were made to within 400 [mu]m of the wall, providing cycle-resolved profiles of the temperature and the random temperature fluctuations. The combustor-cycle phase relationships among the gas temperature, random-temperature-fluctuation intensity, velocity, and combustion chamber pressure, are compared. Velocity field effects dominated the cyclic behavior of the gas temperature, over the effects of isentropic compressive heating and the convection of hot pockets of gas from the combustion chamber. Cycle-resolved profiles show the gas temperature to be constant across the tail pipe, except for a boundary layer region, at all times during the cycle. Although cyclic temperature oscillations of more than 200 K were observed, the thermal boundary layer was well developed at all times during the cycle. The gas temperature was greater than the wall temperature at all cycle times, unlike the reversing velocity field, indicating that Reynolds analogy between momentum and thermal transport is not valid in this flow. Time-resolved wall heat flux was also measured and its fundamental oscillation is found to be in phase with that of the gas temperature.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28549/1/0000348.pd

Time-resolved velocities and turbulence in the oscillating flow of a pulse combustor tail pipe

The cyclic behavior of the oscillating velocity field in the tail pipe of a pulse combustor was studied using laser doppler velocimetry. In this flow, the oscillations result from an acoustic resonance and have amplitudes of up to 5 times the mean velocity. Oscillation frequencies were varied from 67 to 101 Hz. Streamwise velocity and turbulence-intensity boundary layer profiles were measured to within 130 [mu]m of the wall, and transverse turbulence measurements were made to within 2 mm. The phase relationships of the velocity, turbulence intensity, and combustion chamber pressure oscillations are compared. Velocity oscillations near the wall are found to phase lead those in the center of the pipe, creating periodic flow reversals through the boundary layer. A comparison is made between this turbulent oscillating boundary layer and the laminar oscillating boundary layer for flow over a flat plate. The effects of axial position, pulsation frequency, pulsation amplitude, and mean flow rate on the velocity and turbulence profiles are discussed. Time-resolved wall shear stresses (directly calculated from the velocity measurements) are presented and compared with those of steady turbulent flow. Time-averaged velocity and turbulence profiles are also compared with those of conventional steady turbulent flows. The time-averaged velocity profile is found to be flatter than that of steady flow at the same mean Reynolds number, and both the streamwise and transverse turbulence intensities are found to be significantly higher than those of steady flow.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29484/1/0000570.pd

Heat transfer enhancement in the oscillating turbulent flow of a pulse combustor tail pipe

Heat transfer rates in pulse combustor tail pipes and in other reversing, oscillating, turbulent flows have been found to be much higher than those of steady turbulent flow. To elucidate the mechanisms of the enhancement, the temperature and velocity fields, measured with two-line atomic fluorescence (TLAF) and laser Doppier velocimetry (LDV), respectively, are compared. Time-resolved wall heat fluxes and Nusselt numbers are also presented and discussed. Possible causes for the heat transfer enhancement in oscillating flows are reviewed and discussed in view of the data presented in this paper and the recent literature.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29892/1/0000246.pd

Pulse combustion: The importance of characteristic times

The response of a valved pulse combustor to changes in the relative timing between the resonant pressure wave and the instantaneous energy release rate has been examined. Experiments were designed to examine the pulse combustor's response to independent changes in the experimental conditions that resulted in nearly independent changes in the fluid dynamic species mixing time, the fluid dynamic mixing time of cold reactants with hot products, the characteristic chemical kinetics time, and the characteristic resonance time. The time scales considered in this study were adjusted independently to modify the coupling between the instantaneous energy release rate and the resonant pressure wave, thereby modifying the magnitude of the pressure oscillations and altering the frequency of operation. All of these experimental observations of the pulse combustor response to variations in characteristic time scales are interpreted in terms of Rayleigh's criterion.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28111/1/0000560.pd

Evaluation of three 3ABC ELISAs for foot-and-mouth disease non-structural antibodies using latent class analysis

BACKGROUND: Foot-and-mouth disease (FMD) is a highly contagious viral disease of even-toed ungulates. Serological diagnosis/surveillance of FMD presents several problems as there are seven serotypes worldwide and in the event of vaccination it may be necessary to be able to identify FMD infected/exposed animals irrespective of their vaccination status. The recent development of non-structural 3ABC protein (NSP) ELISA tests has greatly advanced sero-diagnosis/surveillance as these tests detect exposure to live virus for any of the seven serotypes of FMD, even in vaccinated populations. This paper analyses the performance of three NSP tests using a Bayesian formulation of the Hui-Walter latent class model to estimate test sensitivity and specificity in the absence of a "gold-standard" test, using sera from a well described cattle population in Cameroon with endemic FMD. RESULTS: The analysis found a high sensitivity and specificity for both the Danish C-ELISA and the World Organisation for Animal Health (O.I.E.) recommended South American I-ELISA. However, the commercial CHEKIT kit, though having high specificity, has very low sensitivity. The results of the study suggests that for NSP ELISAs, latent class models are a useful alternative to the traditional approach of evaluating diagnostic tests against a known "gold-standard" test as imperfections in the "gold-standard" may give biased test characteristics. CONCLUSION: This study demonstrates that when applied to naturally infected zebu cattle managed under extensive rangeland conditions, the FMD ELISAs may not give the same parameter estimates as those generated from experimental studies. The Bayesian approach allows for full posterior probabilities and capture of the uncertainty in the estimates. The implications of an imperfect specificity are important for the design and interpretation of sero-surveillance data and may result in excessive numbers of false positives in low prevalence situations unless a follow-up confirmatory test such as the enzyme linked immunoelectrotransfer blot (EITB) is used

Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania

Background: Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. Methods and findings: Bayesian latent class analysis was used to evaluate performance of the Rose Bengal plate test (RBT) and a competitive ELISA (cELISA) in detecting Brucella spp. exposure at the individual animal-level for cattle, sheep, and goats in Tanzania. Median posterior estimates of RBT sensitivity were: 0.779 (95% Bayesian credibility interval (BCI): 0.570–0.894), 0.893 (0.636–0.989), and 0.807 (0.575–0.966), and for cELISA were: 0.623 (0.443–0.790), 0.409 (0.241–0.644), and 0.561 (0.376–0.713), for cattle, sheep, and goats, respectively. Sensitivity BCIs were wide, with the widest for cELISA in sheep. RBT and cELISA median posterior estimates of specificity were high across species models: RBT ranged between 0.989 (0.980–0.998) and 0.995 (0.985–0.999), and cELISA between 0.984 (0.974–0.995) and 0.996 (0.988–1). Each species model generated seroprevalence estimates for two livestock subpopulations, pastoralist and non-pastoralist. Pastoralist seroprevalence estimates were: 0.063 (0.045–0.090), 0.033 (0.018–0.049), and 0.051 (0.034–0.076), for cattle, sheep, and goats, respectively. Non-pastoralist seroprevalence estimates were below 0.01 for all species models. Series and parallel diagnostic approaches were evaluated. Parallel outperformed a series approach. Median posterior estimates for parallel testing were ≥0.920 (0.760–0.986) for sensitivity and ≥0.973 (0.955–0.992) for specificity, for all species models. Conclusions: Our findings indicate that Brucella spp. surveillance in Tanzania using RBT and cELISA in parallel at the animal-level would give high test performance. There is a need to evaluate strategies for implementing parallel testing at the herd- and flock-level. Our findings can assist in generating robust Brucella spp. exposure estimates for livestock in Tanzania and wider sub-Saharan Africa. The adoption of locally evaluated robust diagnostic tests in setting-specific surveillance is an important step towards brucellosis prevention and control

Diesel Combustion: An Integrated View Combining Laser Diagnostics, Chemical Kinetics, And Empirical Validation

This paper proposes a structure for the diesel combustion process based on a combination of previously published and new results. Processes are analyzed with proven chemical kinetic models and validated with data from production-like direct injection diesel engines. The analysis provides new insight into the ignition and particulate formation processes, which combined with laser diagnostics, delineates the two-stage nature of combustion in diesel engines. Data are presented to quantify events occurring during the ignition and initial combustion processes that form soot precursors. A framework is also proposed for understanding the heat release and emission formation processes

Latent class evaluation of the performance of serological tests for exposure to Brucella spp. in cattle, sheep, and goats in Tanzania

BACKGROUND : Brucellosis is a neglected zoonosis endemic in many countries, including regions of sub-Saharan Africa. Evaluated diagnostic tools for the detection of exposure to Brucella spp. are important for disease surveillance and guiding prevention and control activities. METHODS AND FINDINGS : Bayesian latent class analysis was used to evaluate performance of the Rose Bengal plate test (RBT) and a competitive ELISA (cELISA) in detecting Brucella spp. exposure at the individual animal-level for cattle, sheep, and goats in Tanzania. Median posterior estimates of RBT sensitivity were: 0.779 (95% Bayesian credibility interval (BCI): 0.570–0.894), 0.893 (0.636–0.989), and 0.807 (0.575–0.966), and for cELISA were: 0.623 (0.443–0.790), 0.409 (0.241–0.644), and 0.561 (0.376–0.713), for cattle, sheep, and goats, respectively. Sensitivity BCIs were wide, with the widest for cELISA in sheep. RBT and cELISA median posterior estimates of specificity were high across species models: RBT ranged between 0.989 (0.980–0.998) and 0.995 (0.985–0.999), and cELISA between 0.984 (0.974–0.995) and 0.996 (0.988–1). Each species model generated seroprevalence estimates for two livestock subpopulations, pastoralist and non-pastoralist. Pastoralist seroprevalence estimates were: 0.063 (0.045–0.090), 0.033 (0.018–0.049), and 0.051 (0.034–0.076), for cattle, sheep, and goats, respectively. Non-pastoralist seroprevalence estimates were below 0.01 for all species models. Series and parallel diagnostic approaches were evaluated. Parallel outperformed a series approach. Median posterior estimates for parallel testing were ≥0.920 (0.760-0.986) for sensitivity and ≥0.973 (0.955-0.992) for specificity, for all species models. CONCLUSIONS : Our findings indicate that Brucella spp. surveillance in Tanzania using RBT and cELISA in parallel at the animal-level would give high test performance. There is a need to evaluate strategies for implementing parallel testing at the herd- and flock-level. Our findings can assist in generating robust Brucella spp. exposure estimates for livestock in Tanzania and wider sub-Saharan Africa. The adoption of locally evaluated robust diagnostic tests in setting-specific surveillance is an important step towards brucellosis prevention and control.The UK Biotechnology and Biological Sciences Research Council (BBSRC), Department for International Development (DFID), the Economic & Social Research Council (ESRC), the Medical Research Council (MRC), the Natural Environment Research Council (NERC) and the Defence Science & Technology Laboratory under the Zoonoses and Emerging Livestock Systems programme, Zoonoses and Emerging Livestock Systems – Associated Studentship programme, US National Institutes of Health-National (NIH) Science Foundation Ecology and Evolution of Infectious Disease program, UK BBSRC , the US NIH, National Institute of Allergy and Infectious Diseases through Investigating Febrile Deaths in Tanzania (INDITe) and the BBSRC Institute Strategic Programme Grants.https://journals.plos.org/plosntdspm2022Veterinary Tropical Disease