Development of a radiative transport based, fluorescence-enhanced, frequency-domain small animal imaging system

Herein we present the development of a fluorescence-enhanced, frequency-domain radiative transport reconstruction system designed for small animal optical tomography. The system includes a time-dependent data acquisition instrument, a radiative transport based forward model for prediction of time-dependent propagation of photons in small, non-diffuse volumes, and an algorithm which utilizes the forward model to reconstruct fluorescent yields from air/tissue boundary measurements. The major components of the instrumentation include a charge coupled device camera, an image intensifier, signal generators, and an optical switch. Time-dependent data were obtained in the frequency-domain using homodyne techniques on phantoms with 0.2% to 3% intralipid solutions. Through collaboration with Transpire, Inc., a fluorescence-enhanced, frequency-domain, radiative transport equation (RTE) solver was developed. This solver incorporates the discrete ordinates, source iteration with diffusion synthetic acceleration, and linear discontinuous finite element differencing schemes, to predict accurately the fluence of excitation and emission photons in diffuse and transport limited systems. Additional techniques such as the first scattered distributed source method and integral transport theory are used to model the numerical apertures of fiber optic sources and detectors. The accuracy of the RTE solver was validated against diffusion and Monte Carlo predictions and experimental data. The comparisons were favorable in both the diffusion and transport limits, with average errors of the RTE predictions, as compared to experimental data, typically being less than 8% in amplitude and 7% in phase. These average errors are similar to those of the Monte Carlo and diffusion predictions. Synthetic data from a virtual mouse were used to demonstrate the feasibility of using the RTE solver for reconstructing fluorescent heterogeneities in small, non-diffuse volumes. The current version of the RTE solver limits the reconstruction to one iteration and the reconstruction of marginally diffuse, frequency-domain experimental data using RTE was not successful. Multiple iterations using a diffusion solver successfully reconstructed the fluorescent heterogeneities, indicating that, when available, multiple iterations of the RTE based solver should also reconstruct the heterogeneities

Supercritical Water Gasification: Practical Design Strategies and Operational Challenges for Lab-Scale, Continuous Flow Reactors

Optimizing an industrial-scale supercritical water gasification process requires detailed knowledge of chemical reaction pathways, rates, and product yields. Laboratory-scale reactors are employed to develop this knowledge base. The rationale behind designs and component selection of continuous flow, laboratory-scale supercritical water gasification reactors is analyzed. Some design challenges have standard solutions, such as pressurization and preheating, but issues with solid precipitation and feedstock pretreatment still present open questions. Strategies for reactant mixing must be evaluated on a system-by-system basis, depending on feedstock and experimental goals, as mixing can affect product yields, char formation, and reaction pathways. In-situ Raman spectroscopic monitoring of reaction chemistry promises to further fundamental knowledge of gasification and decrease experimentation time. High-temperature, high-pressure spectroscopy in supercritical water conditions is performed, however, long-term operation flow cell operation is challenging. Comparison of Raman spectra for decomposition of formic acid in the supercritical region and cold section of the reactor demonstrates the difficulty in performing quantitative spectroscopy in the hot zone. Future designs and optimization of SCWG reactors should consider well-established solutions for pressurization, heating, and process monitoring, and effective strategies for mixing and solids handling for long-term reactor operation and data collection

Measurement of gamma-ray spectra from thermal-neutron capture

"AFCRL-63-341."Originally presented as the first author's Ph. D. thesis, Massachusetts Institute of Technology, Dept. of Nuclear Engineering, 1963Scientific report no. 1 for Contract no. AF19(604)-7492Project 4608; Task 46080

Investigation of elemental analysis using neutron-capture gamma ray spectra

"September 1969.""Prepared for United States Department of the Interior Bureau of Mines Morgantown Research Center Morgantown, West Virginia."Also issued as a Ph. D. thesis, written by the first author and supervised by the second author, MIT, Dept. of Nuclear Engineering, 1969Includes bibliographical references (pages 307-312)This thesis evaluated the potential of neutron-capture gamma rays in elemental analysis. A large portion of the work was devoted to the development of a method for the analysis of weak peaks in gamma ray spectra. This was based on equations developed for the standard deviation in the measurement of the various peak parameters, consideration being also given to the reduction in the statistical fluctuations obtained by smoothing the data with the use of Fourier transforms. Two methods of peak area determination were considered end their relative effectiveness examined. An equation was then derived for the minimum weight of an element needed for reliable quantitative analysis. The equations were verified using both real and pseudo-experimental data constructed with the use of a computer. Experiments were carried out using the MIT Reactor with samples positioned La) in a high neutron flux next to the reactor tank (2xl01- n/sq.cm sec), and (b) in an external neutron beam facility of relatively lower but well thermalized flux (2xl0 n/sq.cm sec). Capture gamma ray spectra were obtained with a three-crystal system capable of operating in the free mode, the Compton suppression mode and as a pair spectrometer. The results were used to examine the relative analytical sensitivity of the internal and external sample arrangements and the various gamma detection modes. The minimum measurable weights of 75 elements were evaluated for a stainless steel sample. For these computations use was made of the listing of capture gamma ray spectra recently established by the MIT gamma spectroscopy group. 'In a majority of the cases the detection limits range between 0.1 percent and 10 percent. Equations were developed for extending the results to different samples and different. experimental arrangements.Contract no. H018089

Marine Reptiles

Of the more than 12,000 species and subspecies of extant reptiles, about 100 have re-entered the ocean. Among them are seven species of sea turtles and about 80 species and subspecies of sea snakes, as well as a few other species that are occasionally or regularly found in brackish waters, including various other snakes, the saltwater crocodile, and the marine iguana of the Galapagos Islands. The largest group of marine reptiles, the sea snakes, occur in the tropical and subtropical waters of the Indian and Pacific Oceans from the east coast of Africa to the Gulf of Panama. They inhabit shallow waters along coasts, around islands and coral reefs, river mouths and travel into rivers more than 150 km away from the open ocean. A single species has been found more than 1000 km up rivers. Some have also been found in lakes. The taxonomic status of the sea snakes is still under review and no general agreement exists at the moment. The effects of the exploitation on sea snakes have been investigated in the Philippines and Australia but are almost unknown from other areas. Investigations indicate that some populations are already extinct and others are in danger of extinction in various parts of Asia. All sea turtles are endangered except one. The marine iguana of the Galapagos Islands remains vulnerable due to its limited range. Brackish water snakes are closely associated with mangrove forests and as such are subject to deforestation and coastal development schemes that result in habitat loss. In addition, some are collected for their skins. While none of the coastal species are considered in danger of extinction at the present time, many are data deficient

An Improved Trajectory Model to Evaluate the Collection Performance of Snow Gauges

Recent studies have used numerical models to estimate the collection efficiency\ud of solid precipitation gauges when exposed to the wind, in both\ud shielded and unshielded configurations. The models used computational fluid\ud dynamics (CFD) simulations of the airflow pattern generated by the aerodynamic\ud response to the gauge/shield geometry. These are used as initial conditions\ud to perform Lagrangian tracking of solid precipitation particles. Validation\ud of the results against field observations yielded similarities in the overall\ud behavior, but the model output only approximately reproduced the dependence\ud of the experimental collection efficiency on wind speed. This paper\ud presents an improved snowflake trajectory modeling scheme due to the inclusion\ud of a dynamically-determined drag coefficient. The drag coefficient\ud was estimated using the local Reynolds number as derived from CFD simulations\ud within a time-independent Reynolds Averaged Navier-Stokes (RANS)\ud approach. The proposed dynamic model greatly improves the consistency of\ud results with the field observations recently obtained at the Marshall, CO Winter\ud Precipitation Testbed

Plasma Cytokines/Chemokines as Predictive Biomarkers For Lymphedema in Breast Cancer Patients

Breast cancer-related lymphedema (BCRL) occurs in ~ 40% of patients after axillary lymph node dissection (ALND), radiation therapy (RT), or chemotherapy. First-line palliative treatment utilizes compression garments and specialized massage. Reparative microsurgeries have emerged as a second-line treatment, yet both compression and surgical therapy are most effective at early stages of LE development. Identifying patients at the highest risk for BCRL would allow earlier, more effective treatment. Perometric arm volume measurements, near-infrared fluorescent lymphatic imaging (NIRF-LI) data, and blood were collected between 2016 and 2021 for 40 study subjects undergoing treatment for breast cancer. Plasma samples were evaluated using MILLIPLEX human cytokine/chemokine panels at pre-ALND and at 12 months post-RT. A Mann-Whitne

Lymphatic Dissemination and Axillary Web Syndrome in Primary Cutaneous Tuberculosis Secondary to Needlestick Injury

Cutaneous tuberculosis secondary to skin inoculation of Mycobacterium tuberculosis is uncommon but it can occur in the health care settings. Herein, we report an unusual case of primary cutaneous tuberculosis of the thumb following a needlestick injury. The infection progressed with a necrotic granuloma, lymphatic dysfunction as visualized by near-infrared fluorescence lymphatic imaging, and the development of an axillary web syndrome

Synchronous timing of abrupt climate changes during the last glacial period.

Abrupt climate changes during the last glacial period have been detected in a global array of palaeoclimate records, but our understanding of their absolute timing and regional synchrony is incomplete. Our compilation of 63 published, independently dated speleothem records shows that abrupt warmings in Greenland were associated with synchronous climate changes across the Asian Monsoon, South American Monsoon, and European-Mediterranean regions that occurred within decades. Together with the demonstration of bipolar synchrony in atmospheric response, this provides independent evidence of synchronous high-latitude-to-tropical coupling of climate changes during these abrupt warmings. Our results provide a globally coherent framework with which to validate model simulations of abrupt climate change and to constrain ice-core chronologies