33 research outputs found

    Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

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    Laboratory studies were carried out to assess the bacterial population dynamics and distribution in composite soil samples collected from five (5) different automobile workshops at various locations (Ikpa road, Nwaniba road, Udi street, Idakokpo lane and Mechanic village) within Uyo metropolis. The hydrocarbon utilizing bacteria were isolated and characterized from the soils of automobile workshops using cultural techniques. The total heterotrophic bacterial count (THBC) ranged from 2.5 × 105 to 8.0 × 105 CFU/g of soil sample, while the hydrocarbon utilizing bacterial count (HUBC) ranged from 2.5 × 104 to 4.4 × 104 CFU/g of soil sample. The bacterial isolates which were both Gram positive and Gram negative belonged to the genera; Bacillus, Flavobacterium, Achromobacter, Micrococcus, Citrobacter and Acinetobacter. The total heterotrophic bacterial count (THBC) and hydrocarbon utilizing bacterial count (HUBC) were higher in Ikpa road automobile workshop and Nwaniba road automobile workshops respectively. Bacillus species was found to be present in all the soil samples analysed thus had the highest frequency 5(28%) of occurrence while Achromobacter species had the lowest frequency 1(6%) of occurrence. The presence of these organisms in soils contaminated with spent and unspent lubricating oil and their subsequent growth in enrichment medium supplemented with 1% spent lubricating oil suggest their hydrocarbon utilizing potential, hence, their possible use for the bioremediation of soils impacted with lubricating oil.Keywords: automobile workshops, hydrocarbon utilizing bacteria, bioremediation, enrichment medium,lubricating oi

    Using Web Search Query Data to Monitor Dengue Epidemics: A New Model for Neglected Tropical Disease Surveillance

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    A variety of obstacles, including bureaucracy and lack of resources, delay detection and reporting of dengue and exist in many countries where the disease is a major public health threat. Surveillance efforts have turned to modern data sources such as Internet usage data. People often seek health-related information online and it has been found that the frequency of, for example, influenza-related web searches as a whole rises as the number of people sick with influenza rises. Tools have been developed to help track influenza epidemics by finding patterns in certain web search activity. However, few have evaluated whether this approach would also be effective for other diseases, especially those that affect many people, that have severe consequences, or for which there is no vaccine. In this study, we found that aggregated, anonymized Google search query data were also capable of tracking dengue activity in Bolivia, Brazil, India, Indonesia and Singapore. Whereas traditional dengue data from official sources are often not available until after a long delay, web search query data is available for analysis within a day. Therefore, because it could potentially provide earlier warnings, these data represent a valuable complement to traditional dengue surveillance

    Prevalence and risk factors of malaria among children in southern highland Rwanda

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    <p>Abstract</p> <p>Background</p> <p>Increased control has produced remarkable reductions of malaria in some parts of sub-Saharan Africa, including Rwanda. In the southern highlands, near the district capital of Butare (altitude, 1,768 m), a combined community-and facility-based survey on <it>Plasmodium </it>infection was conducted early in 2010.</p> <p>Methods</p> <p>A total of 749 children below five years of age were examined including 545 randomly selected from 24 villages, 103 attending the health centre in charge, and 101 at the referral district hospital. Clinical, parasitological, haematological, and socio-economic data were collected.</p> <p>Results</p> <p><it>Plasmodium falciparum </it>infection (mean multiplicity, 2.08) was identified by microscopy and PCR in 11.7% and 16.7%, respectively; 5.5% of the children had malaria. PCR-based <it>P. falciparum </it>prevalence ranged between 0 and 38.5% in the villages, and was 21.4% in the health centre, and 14.9% in the hospital. Independent predictors of infection included increasing age, low mid-upper arm circumference, absence of several household assets, reported recent intake of artemether-lumefantrine, and chloroquine in plasma, measured by ELISA. Self-reported bed net use (58%) reduced infection only in univariate analysis. In the communities, most infections were seemingly asymptomatic but anaemia was observed in 82% and 28% of children with and without parasitaemia, respectively, the effect increasing with parasite density, and significant also for submicroscopic infections.</p> <p>Conclusions</p> <p><it>Plasmodium falciparum </it>infection in the highlands surrounding Butare, Rwanda, is seen in one out of six children under five years of age. The abundance of seemingly asymptomatic infections in the community forms a reservoir for transmission in this epidemic-prone area. Risk factors suggestive of low socio-economic status and insufficient effectiveness of self-reported bed net use refer to areas of improvable intervention.</p
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