Aligment of reported lacasses sequences and Lac1 and Lac2 peptides.

<p>(a) Alignment of Lap1 (UniProtKB/Swiss-Prot Q8TG93) and Lap2 (UniProtKB/Swiss-Prot Q8TG94) from <i>T</i><i>. pubescens</i> CBS 696.94. (b) Alignment of Lac1 and Lac2 peptides with Lap1 and Lap2, respectively, and tryptic peptides described by Shleev et al 2007. Alignments were carried out using Clustal Omega and figures were using CLC Sequence Viewer. Different residues are colored in red.</p

Effect of temperature (a) and pH (b) on laccase activity for the UF retentate obtained by semisolid culture of <i>T</i><i>. pubescens</i> on coffee husk (□), soybean pod husk (◊), and cedar sawdust (Δ).

<p>Laccase stability was evaluated by using ABTS. All the assays were performed in triplicate.</p

Laccase activity profiles during <i>T</i><i>. pubescens</i> CBS 696.94 culture under SmC (a) and SSC (b) on coffee husk.

<p>Culture media was enriched with different copper concentrations: 0 (Δ), 0.5 (□), 2 (○) and 5 (◊) mM, and laccase activity was determined by using syringaldazine as substrate. Filled symbols refer to the glucose concentration at the respective copper concentration. All the assays were performed in triplicate.</p

Laccase stability profiles at optimal pH and temperature conditions for crude extracts (solid line), Lac1 (dashed line), and Lac2 (spotted line) obtained from semisolid culture of <i>T</i><i>. pubescens</i> on coffee husk (◊) and cedar sawdust (•).

<p>Laccase stability was evaluated by using ABTS. All the assays were performed in triplicate.</p

Effect of temperature (■) and pH (□) on enzyme activity for Lac1 (solid line) and Lac2 (dashed line), obtained after the purification of the enzymatic crude extract produced by semisolid culture of <i>T</i><i>. pubescens</i> on coffee husk (a), soybean pod husk (b) and cedar sawdust (c).

<p>Laccase stability was evaluated by using ABTS. All the assays were performed in triplicate.</p

Purification of lacasses produced by submerged culture of <i>T</i><i>. pubescens</i> using coffee husk as substrate.

<p>All samples were analyzed by SDS-PAGE under non-reducing conditions and stained with ABTS. No heat denaturation of the samples was conducted. (<b>a</b>) The crude extract (1) was centrifuged and filtered through a Whatman No. 1 filter (2), 0.45 µM membrane (3) 0.22 µm membrane (4), and ultrafiltrated through a 10 kDa cut-off membrane, permeate (5) and retentate (6). (<b>b</b>) UF retentate was purified by anionic exchange chromatography. Lac1 represent the unbound fraction, while Lac2 represent the eluted fraction. The arrow shows the point when protein elution was started. (<b>c</b>) Purified fractions (1) crude extract, (2) Lac1 and (3) Lac2. MW: Molecular Weight. Similar results were observed for crude extracts produced by using soybean pod husk and cedar sawdust.</p

A new copper(I) coordination polymer from 2,6-bis(1<i>H</i>-benzotriazol-1-ylmethyl)pyridine: Synthesis, characterization, and use as additive in transparent submicron UV filters

<p>The use of a new copper(I) coordination polymer (CP) as additive in transparent composite films of 190 nm of thickness for ultraviolet (UV) shielding is presented. The luminescent 1-D Cu(I) CP was easily synthesized through a self-assembly process between Cu(I) iodide and 2,6-bis(1<i>H</i>-benzotriazol-1-ylmethyl)pyridine (L). The CP, [Cu₂(<i>μ</i> − I)₂(<i>μ</i> − <b>L</b>)₂]_<i>n</i>, was structurally characterized by infrared, UV–visible diffuse reflectance and photoluminescence spectroscopy, elemental and thermogravimetric analyses, single-crystal and powder X-ray diffraction, and relativistic density functional theory calculations. The CP was dispersed and immobilized into a polymeric matrix in the presence of Sudan I, yielding a composite material that exhibits a reduction of 49% of the UV transmittance at 350 nm. Thus, the use of a new Cu(I) CP in polymeric composite films appears as a novel approach toward ultrathin and transparent UV shielding films, which have potential applications as protection layers of paints and coatings that tend to degrade when exposed to UV radiation.</p