Eukaryotic gene invasion by a bacterial mobile insertion sequence element IS2 during cloning into a plasmid vector

Escherichia coli (E. coli) are commonly used as hosts for DNA cloning and sequencing. Upon transformation of E. coli with recombined vector carrying a gene of interest, the bacteria multiply the gene of interest while maintaining the integrity of its content. During the subcloning of a mouse genomic fragment into a plasmid vector, we noticed that the size of the insert increased significantly upon replication in E. coli. The sequence of the insert was determined and found to contain a novel DNA sequence within the mouse genomic insert. A BLAST search of GenBank revealed the novel sequence to be that of the Insertion Sequence 2 (IS2) element from E. coli that was likely inserted during replication in that organism. Importantly, a detailed search of GenBank shows that the IS2 is present within many eukaryotic nucleotide sequences, and in many cases, has been annotated as being part of the protein. The results of this study suggest that one must perform additional careful analysis of the sequence results using BLAST comparisons, and further verification of gene annotation before submission into the GenBank

Changes and demands in the higher education sector are increasingly making advanced degree medical physics programs nonviable and the profession will have to develop a new model for delivering such education

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141195/1/mp12645_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141195/2/mp12645.pd

Βασικές αρχές επαγγελματικής συμβουλευτικής: Διαδικασίες και τεχνικές

Το εγχειρίδιο αυτό αποτελεί την ελληνική έκδοση του “Essential Elements of Career Counseling, Processes and Techniques” των Norman Amundson, JoAnn Harris – Bowlsbey, Spencer G. Niles, (Pearson Merrill Prentice Hall, Colombus Ohio, second Edition 2008). Μεταφράστηκε και εκδόθηκε από το Εθνικό Κέντρο Επαγγελματικού Προσανατολισμού (ΕΚΕΠ) στο πλαίσιο του ρόλου του ως ο εθνικός φορέας συντονισμού, υποστήριξης και ενδυνάμωσης της δράσης των Λειτουργών Συμβουλευτικής και Επαγγελματικού Προσανατολισμού στην εκπαίδευση, την κατάρτιση και την απασχόληση. Το βιβλίο διαπραγματεύεται βασικά ζητήματα τα οποία διαχειρίζονται οι σύμβουλοι σταδιοδρομίας κατά τη διάρκεια της συμβουλευτικής διαδικασίας. Ως στόχο έχει να προσφέρει τη δυνατότητα σε κάθε σύμβουλο να αποκτήσει μία ολοκληρωμένη εικόνα για τις μεθόδους και τεχνικές που μπορούν να αξιοποιηθούν στο πλαίσιο της επαγγελματικής συμβουλευτικής, σε ατομικό ή ομαδικό επίπεδο. Παρουσιάζονται επίσης βασικές θεωρίες επαγγελματικής συμβουλευτικής, με έμφαση στην πρακτική αξιοποίησή τους, η οποία αναδεικνύεται μέσω αντιπροσωπευτικών μελετών περίπτωσης. Το ΕΚΕΠ διαθέτει το παρόν εγχειρίδιο σε στελέχη και φορείς που υλοποιούν δράσεις Συμβουλευτικής και Επαγγελματικού Προσανατολισμού, ευελπιστώντας ότι θα αποτελέσει ένα ουσιαστικό βοήθημα στο έργο τους και θα συμβάλλει στην ποιοτική αναβάθμιση των υπηρεσιών Επαγγελματικού Προσανατολισμού

Effect of Cracking on Chloride Content in Concrete Bridge Decks

Field surveys to measure bridge deck cracking and chloride contents of uncracked as well as cracked concrete were performed as a part of a larger research program evaluating bridge deck performance. Three deck types were studied: monolithic decks, decks with a conventional high density concrete overlay, and decks with a high density concrete overlay containing either a 5 or 7% replacement of cement by silica fume. The results of the field surveys indicate that bridge deck type does not have a major effect on chloride content. For samples taken away from cracks, the average chloride concentration at the top of transverse reinforcement rarely exceeded even the most conservative estimates of the corrosion threshold for conventional reinforcement. Chloride concentrations taken at crack locations, however, often exceeded the corrosion threshold of conventional reinforcement in less than 1 year

Effect of Combination Folic Acid, Vitamin B6 , and Vitamin B12 Supplementation on Fracture Risk in Women: A Randomized, Controlled Trial.

Epidemiologic studies have demonstrated an association of elevated plasma homocysteine levels with greater bone resorption and fracture risk. Vitamins B12 , B6 , and folic acid are cofactors in homocysteine metabolism, and supplementation with B vitamins is effective in lowering homocysteine levels in humans. However, randomized trials of supplemental B vitamins for reduction of fracture risk have been limited. Therefore, we performed an ancillary study to the Women's Antioxidant and Folic Acid Cardiovascular Study (WAFACS), a large randomized trial of women with preexisting cardiovascular disease or three or more coronary risk factors, to test whether a daily B vitamin intervention including folic acid (2.5 mg/day), vitamin B6 (50 mg/day), and vitamin B12 (1 mg/day) reduces nonspine fracture risk over 7.3 years of treatment and follow-up. Among 4810 women, we confirmed 349 nonspine fracture cases by centralized review of medical records. In a substudy of 300 women (150 in treatment group and 150 controls) with paired plasma samples at randomization and follow-up (7.3 years later), we measured two bone turnover markers, including C-terminal cross-linking telopeptide of type I collagen (CTX) and intact type I procollagen N-propeptide (P1NP). In Cox proportional hazards models based on intention-to-treat, we found no significant effects of B vitamin supplementation on nonspine fracture risk (relative hazard = 1.08; 95% confidence interval, 0.88 to 1.34). In a nested case-cohort analysis, there were no significant effects of B vitamins on fracture risk among women with elevated plasma homocysteine levels, or low levels of vitamins B12 or B6 , or folate at baseline. Furthermore, treatment with B vitamins had no effect on change in markers of bone turnover. We found no evidence that daily supplementation with B vitamins reduces fracture risk or rates of bone metabolism in middle-aged and older women at high risk of cardiovascular disease. © 2017 American Society for Bone and Mineral Research

The very large G-protein coupled receptor VLGR1: a component of the ankle link complex required for the normal development of auditory hair bundles

Sensory hair bundles in the inner ear are composed of stereocilia that can be interconnected by a variety of different link types, including tip links, horizontal top connectors, shaft connectors, and ankle links. The ankle link antigen is an epitope specifically associated with ankle links and the calycal processes of photoreceptors in chicks. Mass spectrometry and immunoblotting were used to identify this antigen as the avian ortholog of the very large G-protein-coupled receptor VLGR1, the product of the Usher syndrome USH2C (Mass1) locus. Like ankle links, Vlgr1 is expressed transiently around the base of developing hair bundles in mice. Ankle links fail to form in the cochleae of mice carrying a targeted mutation in Vlgr1 (Vlgr1/del7TM), and the bundles become disorganized just after birth. FM1-43 [N-(3-triethylammonium)propyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] dye loading and whole-cell recordings indicate mechanotransduction is impaired in cochlear, but not vestibular, hair cells of early postnatal Vlgr1/del7TM mutant mice. Auditory brainstem recordings and distortion product measurements indicate that these mice are severely deaf by the third week of life. Hair cells from the basal half of the cochlea are lost in 2-month-old Vlgr1/del7TM mice, and retinal function is mildly abnormal in aged mutants. Our results indicate that Vlgr1 is required for formation of the ankle link complex and the normal development of cochlear hair bundles

Opportunity lost: End‐of‐life discussions in cancer patients who die in the hospital

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98330/1/jhm1989.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/98330/2/jhm1989-sup-0001-SuppInfo.pd

Under the Cover Infant Pose Estimation using Multimodal Data

Infant pose monitoring during sleep has multiple applications in both healthcare and home settings. In a healthcare setting, pose detection can be used for region of interest detection and movement detection for noncontact based monitoring systems. In a home setting, pose detection can be used to detect sleep positions which has shown to have a strong influence on multiple health factors. However, pose monitoring during sleep is challenging due to heavy occlusions from blanket coverings and low lighting. To address this, we present a novel dataset, Simultaneously-collected multimodal Mannequin Lying pose (SMaL) dataset, for under the cover infant pose estimation. We collect depth and pressure imagery of an infant mannequin in different poses under various cover conditions. We successfully infer full body pose under the cover by training state-of-art pose estimation methods and leveraging existing multimodal adult pose datasets for transfer learning. We demonstrate a hierarchical pretraining strategy for transformer-based models to significantly improve performance on our dataset. Our best performing model was able to detect joints under the cover within 25mm 86% of the time with an overall mean error of 16.9mm. Data, code and models publicly available at https://github.com/DanielKyr/SMa

Building a Bridge to Next Generation DH Services in Libraries with a Campus Needs Assessment

This poster reports on a needs assessment for digital humanities library services undertaken at large research university in order to provide a basis for transition to a next phase of Digital Humanities (DH) support at a library supporting a growing amount of DH work on campus. It reports key findings and how the library services will evolve to meet needs identified on campus. The full report on which this presentation is based is available at http://hdl.handle.net/2142/100081Ope