338 research outputs found
Triacylglycerol profile as a chemical fingerprint of mushroom species: evaluation by principal component and linear discriminant analyses.
Mushrooms are becoming relevant foods due to their nutritional, gastronomic and
pharmacological properties, namely antioxidant, antitumor and antimicrobial properties.
However, despite several mushroom species have been chemically characterized, the
evaluation of triacylglycerol (TAG) profile remained nearly discarded. Since TAG was
formerly used to assess the authentication of highly valued commercial oils, and the
distribution of fatty acids on the glycerol molecule is genetically controlled, the potential of
TAG profile to act as taxonomical marker was evaluated in 30 wild mushroom species.
Principal component analysis and linear discriminant analysis were used to verify the
taxonomical rank (order, family, genus or species) more related with the detected TAG
profile. The results pointed out that the ability of TAG profile to discriminate mushroom
samples increased for the lower taxonomical ranks, reaching a maximal performance for
species discrimination. Since there is high resemblance among mushroom species belonging
to the same genus and considering that conservation techniques applied to mushrooms often
change their physical properties, this might be considered as a valuable outcome with
important practical applications
Triacylglycerols profile and principal component analysis as a chemometric tool to identify wild edible mushroom genera
The Northeast of Portugal is among the European regions with high divers ity of wild
mushrooms, some with recognized gastronomic value. Due to the high resemblance among
mushroom species belonging to the same genus, finding a distinctive chemometric marker
to identify mushroom species is a mandatory task. The lipidic fraction of a natural product
has a characteristic pattern of triaeylglyccrol (TAG), comprising specific information due
to the genetic control of the stereospecific distribution of fatty acids (FA) on the glycerol
molecule, which is typical for each species. Principal component analysis (PCA) was
previously applied to classify mushroom samples according to their fatty acids profile.121
However, despite TAG profile was used before to assess the authenticity of oils,l31 it was
not used as taxonomical marker. Accordingly, TAG profile was evaluated in 30 wild
mushroom species and the results were classified by PCA to identify their discriminant
ability. TAG profiles obtained by high performance liquid chromatography coupled to an
evaporative light scattering detector showed significant differences among species. The
PCA biplot (1) showed that TAG profiles recorded for different mushroom genera may be
used as an effective differentiating tool, especially when lowering the taxonomical rank,
indicating that TAG profile is most related with the lowest ranks. Ln conclusion, this
chemometric approach might be used as a practical tool to identify mushroom species
Ergosterol contents in mycorrhizal wild edible mushrooms: comparison by hierarchical cluster analysis
Sterols are important molecules of the unsaponifiable fraction in several matrices. In
mushrooms, ergosterol, which is an important vitamin D2 precursor, is clearly the main
sterol. Hierarchical cluster analysis (HCA) is an unsupervised learning method to
standardized data, checking for similarities between sample groups. This method
calculates the distances (or correlation) between all samples using a defined metric such
as squared Euclidean distance or Chebychev distance. Hierarchical clustering is the
most common approach in which clusters are formed sequentially. The most similar
objects are first grouped, and these initial groups are merged according to their
similarities. Eventually as the similarity decreases all subgroups are fused into a single
cluster. Herein, ergosterol was quantified by high performance liquid chromatography,
coupled with an ultraviolet detector, in some of the most appreciated mycorrhizal edible
mushrooms (Amanita caesarea, Boletus edulis, Cantharellus cibarius, Lactarius
deliciosus and Morchella esculenta). Considering fat content (percentage) and
ergosterol contents in mg/g fat and mg/100 g of dry weight, two main groups were
formed in the HCA: one aggregating A. caesarea and B. edulis and another constituted
by C. cibarius, L. deliciosus and M. esculenta. These two groups derived from the
higher contents presented by B. edulis and A. caesarea. The detected values indicate
that mushrooms might act as a potential source of this vitamin D2 precursor, with
special relevance for diets deprived of food products of animal origin.Foundation for Science and Technology (FCT, Portugal) and
COMPETE/QREN/EU for the financial support of this work (research project
PTDC/AGR-ALI/110062/2009) and to CIMO (strategic project PEst-
OE/AGR/UI0690/2011). J.C.M. Barreira also thanks to FCT, POPH-QREN and FSE for
his grant SFRH/BPD/72802/2010
Development of a novel methodology for the analysis of ergosterol in mushrooms
Sterols are important molecules in the unsaponifiable fraction of several matrices. Ergosterol, which is an important vitamin D2 precursor, is clearly the main sterol in mushrooms. Herein, an analytical method for ergosterol determination in cultivated and wild mushrooms was developed using high-performance liquid chromatography coupled to ultraviolet detection. The chromatographic separation was achieved in an Inertsil 100A ODS-3 reversed-phase column using an isocratic elution with acetonitrile/methanol (70:30, v/v) at a flow rate of 1 mL/min. Different extraction methodologies were tested, using n-hexane, methanol/dichloromethane (75:25, v/v) or chloroform/methanol (20:10, v/v). The method was optimised using Fistulina hepatica and proved to be reproductive and accurate. Ergosterol was the most abundant sterol by a greater extent in all mushrooms. In general, the cultivated species showed higher contents, mainly Agaricus bisporus and Lentinus edodes. Among wild species, Boletus edulis was the mushroom with the highest content in ergosterol. Results were expressed in fat content, dry weight and fresh weight bases. The assessment of ergosterol amounts might be very useful due to the bioactive potential that has been attributed to this molecule and its derivatives
Perfil de triacilgliceróis de cogumelos comestÃveis: diferenciação inter-especÃfica por análise discriminante linear
Os cogumelos têm ganho importância como bens alimentares devido à s suas propriedades nutricionais, gastronómicas e farmacológicas. No entanto, apesar de diversas espécies de cogumelos terem já sido caracterizadas quimicamente, a avaliação do seu perfil em triacilgliceróis (TAG) tem sido alvo de menos estudos. O perfil em TAG foi já utilizado para avaliar a autenticidade de óleos comerciais de elevado valor, pelo que também poderá atuar como um potencial marcador taxonómico. Para verificar esta possibilidade, foi feita uma análise discriminante linear (ADL), tentando também definir-se qual o nÃvel taxonómico mais relacionado com o perfil em TAG. Os resultados indicaram que o poder discriminante do perfil em TAG aumenta para os nÃveis taxonómicos mais especÃficos, atingindo um máximo na separação entre espécies. Uma vez que existem cogumelos pertencentes ao mesmo género que apresentam elevada semelhança no seu aspecto, e considerando ainda que as técnicas de conservação aplicadas aos cogumelos alteram muitas vezes as suas caracterÃsticas fÃsicas, os resultados obtidos neste trabalho poderão ter importantes aplicações práticas
Natural phytochemicals and probiotics as bioactive ingredients for functional foods: Extraction, biochemistry and protected-delivery technologies
he well-known correlation between diet and physiology demonstrates the great possibilities of food to maintain or improve our health, increasing the interest in finding new products with positive physiological effects. Nowadays, one of the top research areas in Food Science and Technology is the extraction and characterization of new natural ingredients with biological activity that can be further incorporated into a functional food, contributing to consumer's well-being. Furthermore, there is a high demand for effective encapsulation methodologies to preserve all the characteristics of bioactive compounds until the physiological action site is reached. Scope and approach: In this review, the relevance of developing standard approaches for the extraction of the highly diverse bioactive compounds was described, as it defines the suitability of the following steps of separation, identification and characterization. Special attention was also dedicated to the encapsulation techniques used on hydrophilic and/or lipophilic compounds (e.g., emulsification, coacervation, supercritical fluid, inclusion complexation, emulsification-solvent evaporation and nanoprecipitation). Key findings and conclusions: Some useful conclusions regarding the selection of the best extraction methodology (Soxhlet extraction, ultrasound-assisted extraction, supercritical fluid extraction, accelerated solvent extraction, or shake extraction) were achieved, considering important aspects such as cost, required technical skills, extract integrity, green chemistry principles, solvent type, sample size, pH, temperature and pressure. In addition, this comprehensive review allowed defining the best protective approach to solve the limitations related to the extremely low absorption and bioavailability of bioactive phytochemicals, overcoming problems related to their low solubility, poor stability, low permeability and metabolic processes in the GI tract.info:eu-repo/semantics/publishedVersio
Antioxidant potential of chestnut (Castanea sativa L.) and almond (Prunus dulcis L.) by-products.
The antioxidant properties of almond green husks (Cvs. Duro Italiano, Ferraduel, Ferranhês, Ferrastar and Orelha de Mula), chestnut skins and chestnut leaves (Cvs. Aveleira, Boa Ventura, Judia and Longal) were evaluated through several chemical and biochemical assays in order to provide a novel strategy to stimulate the application of waste products as new suppliers of useful bioactive compounds, namely antioxidants. All the assayed by-products revealed good antioxidant properties, with very low EC50 values (lower than 380 μg/mL), particularly for lipid peroxidation inhibition (lower than 140 μg/mL). The total phenols and flavonoids contents were also determined. The correlation between these bioactive compounds and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation in pig brain tissue through formation of thiobarbituric acid reactive substances, was also obtained. Although, all the assayed by-products proved to have a high potential of application in new antioxidants formulations, chestnut skins and leaves demonstrated better results
Sugars profiles of different chestnut (Castanea sativa Mill.) and almond (Prunus dulcis) cultivars by HPLC-RI
Sugar profiles of different almond and chestnut
cultivars were obtained by high-performance liquid chromatography
(HPLC), by means of a refractive index (RI)
detector. A solid-liquid extraction procedure was used in
defatted and dried samples. The chromatographic separation
was achieved using a Eurospher 100-5 NH2 column using an
isocratic elution with acetonitrile/water (70:30, v/v) at a flow
rate of 1.0 ml/min. All the compounds were separated in
16 min. The method was optimized and proved to be
reproducible and accurate. Generally, more than 95% of
sugars were identified for both matrixes. Sugars profiles
were quite homogeneous for almond cultivars; sucrose was
the main sugar (11.46±0.14 in Marcona to 22.23±0.59 in
Ferragnes g/100 g of dried weight), followed by raffinose
(0.71±0.05 in Ferraduel to 2.11±0.29 in Duro Italiano),
glucose (0.42±0.12 in Pegarinhos two seeded to 1.47±0.19
in Ferragnes) and fructose (0.11±0.02 in Pegarinhos two
seeded to 0.59±0.05 in Gloriette). Commercial cultivars
proved to have higher sucrose contents, except in the case
of Marcona. Nevertheless, chestnut cultivars revealed a
high heterogeneity. Sucrose was the main sugar in Aveleira
(22.05±1.48), Judia (23.30±0.83) and Longal (9.56± 0.91), while glucose was slightly prevalent in Boa Ventura
(6.63±0.49). The observed variance could serve for intercultivar
discrimination
Antioxidant activity and bioactive compounds of ten Portuguese regional and commercial almond cultivars.
The antioxidant properties of different almond cultivars (cv.), either regional (Casanova, Duro Italiano, Molar, Orelha de Mula and Pegarinhos cv.) or commercial (Ferraduel, Ferranhês, Ferrastar and Guara
cv.) were evaluated through several chemical and biochemical assays: DPPH (2,2-diphenyl-1-picrylhydrazyl)
radical scavenging activity, reducing power, inhibition of b-carotene bleaching, inhibition
of oxidative hemolysis in erythrocytes, induced by 2,20-azobis(2-amidinopropane)dihydrochloride
(AAPH), and inhibition of thiobarbituric acid reactive substances (TBARS) formation in brain cells, all used
as models for the lipid peroxidation damage in biomembranes. The EC50 values were calculated for all the
methods in order to evaluate the antioxidant efficiency of each almond cultivar. Bioactive compounds
such as phenols and flavonoids were also obtained and correlated to antioxidant activity. The results
obtained were quite heterogeneous, revealing significant differences among the cultivars assayed. Duro
Italiano cv. revealed better antioxidant properties, presenting lower EC50 values in all assays, and the
highest antioxidants contents. The protective effect of this cultivar on erythrocyte biomembrane hemolysis
was maintained during 4 h
A New Age for Quercus spp. Fruits: Review on Nutritional and Phytochemical Composition and Related Biological Activities of Acorns
The current global food system must adapt to the expected growth of world population (about 9 billion individuals by 2050). This adaptation will probably include an increased consumption of edible wild foods, due to their richness in micronutrients and bioactive compounds, besides providing a cost-effective and sustainable way of improving caloric food security. A striking example of such natural matrices is the Quercus genus, which has the additional advantage of being widespread throughout the Northern Hemisphere. In a traditional sense, Quercus fruits (acorns) were mainly used in animal feeding, despite their potentially important role on the rural economy. But this preconception is changing. In fact, their nutritional value, high contents in phytochemical compounds, biological activity (such as antioxidant, anticarcinogenic, and cardioprotective properties) and use in the treatment of specific diseases (such as atherosclerosis, diabetes, or Alzheimer's disease) have raised the interest in integrating acorns into the human diet. Accordingly, this comprehensive overview was designed to provide an evidence-based review of the literature, with the objective to achieve useful conclusions regarding the nutritional properties, methodologies of extraction, identification, and characterization of a wide variety of bioactive compounds and scientifically validated bioactivities in Quercus species worldwide. The industrial by-products from acorn oil extraction or flour production are also included. Data regarding the analytical techniques, individual compounds, and their bioactivities, are organized in tables. The reported data are discussed and directions for further investigations are suggested, highlighting the use of acorns in food, nutraceutical, and pharmaceutical applications.The authors are grateful for the financial support (UID/QUI/
50006/2013-POCI/01/0145/FEDER/007265) from FCT/
MEC through national funds and cofinanced by FEDER, under
the Partnership Agreement PT2020. J.C.M. Barreira thanks FCT,
POPH-QREN, and FSE for his grant (SFRH/BPD/72802/
2010).
The authors thank Telma Oliveira for drawing the acorn sketch
depicted in Figure 1.info:eu-repo/semantics/publishedVersio
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