13 research outputs found
Vaccine Induced Specific Protection Against Enteric Red Mouth Disease (ERM) Caused by Yersinia Ruckeri Serotype 1 Biotype 2
Oil adjuvant elevates protection of rainbow trout (Oncorhynchus mykiss) following injection vaccination against Yersinia ruckeri
Insight from Molecular, Pathological, and Immunohistochemical Studies on Cellular and Humoral Mechanisms Responsible for Vaccine-Induced Protection of Rainbow Trout against <em>Yersinia ruckeri</em>
The immunological mechanisms associated with protection of vaccinated rainbow trout, Oncorhynchus mykiss, against enteric redmouth disease (ERM), caused by Yersinia ruckeri, were previously elucidated by the use of gene expression methodology and immunochemical methods. That approach pointed indirectly to both humoral and cellular elements being involved in protection. The present study correlates the level of protection in rainbow trout to cellular reactions in spleen and head kidney and visualizes the processes by applying histopathological, immunohistochemical, and in situ hybridization techniques. It was shown that these cellular reactions, which were more prominent in spleen than in head kidney, were associated with the expression of immune-related genes, suggesting a Th2-like response. Y. ruckeri, as shown by in situ hybridization (ISH), was eliminated within a few days in vaccinated fish, whereas nonprotected fish still harbored bacteria for a week after infection. Vaccinated fish reestablished normal organ structure within a few days, whereas nonprotected fish showed abnormalities up to 1 month postinfection. Protection in the early phase of infection was mainly associated with the expression of genes encoding innate factors (complement factors, lysozyme, and acute phase proteins), but in the later phase of infection, increased expression of adaptive immune genes dominated. The histological approach used has shown that the cellular changes correlated with protection of vaccinated fish. They comprised transformation of resident cells into macrophage-like cells and increased occurrence of CD8α and IgM cells, suggesting these cells as main players in protection. Future studies should investigate the causality between these factors and protection
Comparative Efficacies of Sodium Percarbonate, Peracetic Acid, and Formaldehyde for Control of Ichthyobodo Necator—An Ectoparasitic Flagellate from Rainbow Trout
Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model
Oil adjuvant elevates protection of rainbow trout (Oncorhynchus my-kiss) following injection vaccination against Yersinia ruckeri
Øko-foder lige så godt for regnbueørredyngel som konventionelt
Ingredienserne i fiskefoderet ser ud til at have større betydning end hvorvidt foderet er økologisk eller konventionelt ved opdræt af regnbueørreder. Det viser forsøg gennemført i projektet OPTIFISH
Organic diets are equally good for rainbow trout fry as conventional diets
With respect to rainbow trout, the OPTIFISH project has shown that feed composition is more important than whether the feed are of organic or conventional origin