Effect of soil particle-size distribution (PSD) on soil-subsoiler interactions in the discrete element model

Aim of study: This work investigated the significance and mechanism for the effect of particle-size distribution (PSD) under different nominal radii using the discrete element method (DEM) and validated using the laboratory soil-bin results to accurately determine PSD.Area of study: Yangling, ChinaMaterial and methods: The experimental soil was Lou soil. Soil disturbance characteristics (soil rupture distance ratio, height of accumulated soil, soil density change rate) and cutting forces (draft and vertical) under different treatments were predicted and measured respectively.Main results: The ANOVA outputs showed that PSD significantly affected draft and vertical forces (

Identification and analysis of unitary loss of long-established protein-coding genes in Poaceae shows evidences for biased gene loss and putatively functional transcription of relics

BACKGROUND: Long-established protein-coding genes may lose their coding potential during evolution (“unitary gene loss”). Members of the Poaceae family are a major food source and represent an ideal model clade for plant evolution research. However, the global pattern of unitary gene loss in Poaceae genomes as well as the evolutionary fate of lost genes are still less-investigated and remain largely elusive. RESULTS: Using a locally developed pipeline, we identified 129 unitary gene loss events for long-established protein-coding genes from four representative species of Poaceae, i.e. brachypodium, rice, sorghum and maize. Functional annotation suggested that the lost genes in all or most of Poaceae species are enriched for genes involved in development and response to endogenous stimulus. We also found that 44 mutated genomic loci of lost genes, which we referred as relics, were still actively transcribed, and of which 84% (37 of 44) showed significantly differential expression across different tissues. More interestingly, we found that there were totally five expressed relics may function as competitive endogenous RNA in brachypodium, rice and sorghum genome. CONCLUSIONS: Based on comparative genomics and transcriptome data, we firstly compiled a comprehensive catalogue of unitary gene loss events in Poaceae species and characterized a statistically significant functional preference for these lost genes as well showed the potential of relics functioning as competitive endogenous RNAs in Poaceae genomes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12862-015-0345-x) contains supplementary material, which is available to authorized users

Effect of soil particle-size distribution (PSD) on soil-subsoiler interactions in the discrete element model

Aim of study: This work investigated the significance and mechanism for the effect of particle-size distribution (PSD) under different nominal radii using the discrete element method (DEM) and validated using the laboratory soil-bin results to accurately determine PSD.Area of study: Yangling, ChinaMaterial and methods: The experimental soil was Lou soil. Soil disturbance characteristics (soil rupture distance ratio, height of accumulated soil, soil density change rate) and cutting forces (draft and vertical) under different treatments were predicted and measured respectively.Main results: The ANOVA outputs showed that PSD significantly affected draft and vertical forces (

The complete chloroplast genome of Pedicularis alaschanica (Orobanchaceae)

The complete chloroplast genome sequence of Pedicularis alaschanica was determined and described. The complete chloroplast was 146,989 bp in length with typical quadripartite structure and overall GC content of 38.4%, which encompassed 68 protein-coding genes, 22 tRNAs, 4 rRNAs, and 11 pseudogenes. The functions of ndh genes were lost. The phylogenetic analysis indicated that P. alaschanica was close to other species of Pedicularis. This study would contribute to enrich the Pedicularis chloroplast genome resource and promote the biological research

Design and Experimental Research on Soil Covering Device with Linkage and Differential Adjustment of Potato Planter

When the potato planter works on sloping field, it will cause problems such as poor film mulching quality due to the difference in volume of soil covering both sides of the discs and the inconvenient adjustment of the soil covering disc. The soil covering device with linkage and differential adjustment was designed to improve the mulching quality. The main research content includes explaining the structure and principle of the soil covering device and analyzing the structural parameters of the adjustment mechanism. The field experiment was completed to verify the performance of soil covering device, which takes the stability coefficient and uniformity coefficient of the volume of covering soil as factors. The result shows the following: (1) The volume of covering soil changes exponentially with the angle of the disc through data fitting, which can standardize the angle of covering disc; and (2) when the angle of disc is 30° and 60°, respectively, the uniformity coefficient of volume of covering soil is lower than 1.4, which has premium soil covering quality. When the angles of the discs on both sides differ greatly, the stability coefficient of volume of covering soil is 0.41, which can meet the requirements of the mulching quality of potato planter. This research provides the technical support for high-quality potato planting

Herceptin Enhances the Antitumor Effect of Natural Killer Cells on Breast Cancer Cells Expressing Human Epidermal Growth Factor Receptor-2

Optimal adoptive cell therapy (ACT) should contribute to effective cancer treatment. The unique ability of natural killer (NK) cells to kill cancer cells independent of major histocompatibility requirement makes them suitable as ACT tools. Herceptin, an antihuman epidermal growth factor receptor-2 (anti-HER2) monoclonal antibody, is used to treat HER2+ breast cancer. However, it has limited effectiveness and possible severe cardiotoxicity. Given that Herceptin may increase the cytotoxicity of lymphocytes, we explored the possible augmentation of NK cell cytotoxicity against HER2+ breast cancer cells by Herceptin. We demonstrated that Herceptin could interact with CD16 on NK cells to expand the cytotoxic NK (specifically, CD56dim) cell population. Additionally, Herceptin increased NK cell migration and cytotoxicity against HER2+ breast cancer cells. In a pilot study, Herceptin-treated NK cells shrunk lung nodular metastasis in a woman with HER2+ breast cancer who could not tolerate the cardiotoxic side effects of Herceptin. Our findings support the therapeutic potential of Herceptin-treated NK cells in patients with HER2+ and Herceptin-intolerant breast cancer

Genomic Instability in Cerebrospinal Fluid Cell-Free DNA Predicts Poor Prognosis in Solid Tumor Patients with Meningeal Metastasis

Genomic instability (GI), which leads to the accumulation of DNA loss, gain, and rearrangement, is a hallmark of many cancers such as lung cancer, breast cancer, and colon cancer. However, the clinical significance of GI has not been systematically studied in the meningeal metastasis (MM) of solid tumors. Here, we collected both cerebrospinal fluid (CSF) and plasma samples from 56 solid tumor MM patients and isolated cell-free ctDNA to investigate the GI status using a next-generation sequencing-based comprehensive genomic profiling of 543 cancer-related genes. According to the unfiltered heterozygous mutation data-derived GI score, we found that 37 (66.1%) cases of CSF and 3 cases (6%) of plasma had a high GI status, which was further validated by low-depth whole-genome sequencing analysis. It is demonstrated that a high GI status in CSF was associated with poor prognosis, high intracranial pressure, and low Karnofsky performance status scores. More notably, a high GI status was an independent poor prognostic factor of poor MM-free survival and overall survival in lung adenocarcinoma MM patients. Furthermore, high occurrences of the co-mutation of TP53/EGFR, TP53/RB1, TP53/ERBB2, and TP53/KMT2C were found in MM patients with a high GI status. In summary, the GI status in CSF ctDNA might be a valuable prognostic indicator in solid tumor patients with MM