Effects of Multiple Metal Binding Sites on Calcium and Magnesium-dependent Activation of BK Channels

BK channels are activated by physiological concentrations of intracellular Ca2+ and Mg2+ in a variety of cells. Previous studies have identified two sites important for high-affinity Ca2+ sensing between [Ca2+]i of 0.1–100 μM and a site important for Mg2+ sensing between [Mg2+]i of 0.1–10 mM. BK channels can be also activated by Ca2+ and Mg2+ at concentrations >10 mM so that the steady-state conductance and voltage (G-V) relation continuously shifts to more negative voltage ranges when [Mg2+]i increases from 0.1–100 mM. We demonstrate that a novel site is responsible for metal sensing at concentrations ≥10 mM, and all four sites affect channel activation independently. As a result, the contributions of these sites to channel activation are complex, depending on the combination of Ca2+ and Mg2+ concentrations. Here we examined the effects of each of these sites on Ca2+ and Mg2+-dependent activation and the data are consistent with the suggestion that these sites are responsible for metal binding. We provide an allosteric model for quantitative estimation of the contributions that each of these putative binding sites makes to channel activation at any [Ca2+]i and [Mg2+]i

Discussion on Event Horizon and Quantum Ergosphere of Evaporating Black Holes in a Tunnelling Framework

In this paper, with the Parikh-Wilczek tunnelling framework the positions of the event horizon of the Vaidya black hole and the Vaidya-Bonner black hole are calculated respectively. We find that the event horizon and the apparent horizon of these two black holes correspond respectively to the two turning points of the Hawking radiation tunnelling barrier. That is, the quantum ergosphere coincides with the tunnelling barrier. Our calculation also implies that the Hawking radiation comes from the apparent horizon.Comment: 8 page

Solvatochromic Parameters of the Binary Mixtures of Imidazolium Chloride Ionic Liquid Plus Molecular Solvent

Imidazolium-based chloride ionic liquids (ILs) have exhibited remarkable performance in several important applications such as biomass dissolution and extraction, but their large viscosity is a non-negligible problem. Adding molecular co-solvents into chloride ILs is effective in reducing viscosity; nevertheless, understanding of the accompanied change of thermodynamic polarity is quite few. Therefore, in this work we reported three Kamlet-Taft solvatochromic parameters, including dipolarity/polarizability π*), hydrogen-bond acidity (α) and hydrogen-bond basicity (β), for the binary mixtures of several imidazolium-based chloride ILs plus either dipolar protic solvents (water and methanol) or dipolar aprotic solvents (dimethyl sulfoxide, N,N-dimethylformamide and acetonitrile). The results demonstrated that those parameters could be altered by the structure of IL and type of co-solvent owing to the solute-solvent and solvent-solvent interactions. The structure of alkyl chain of cation had considerable impact on the π* variation of IL aqueous solution against IL concentration but hardly affected other mixtures. Moreover, remarkable preferential solvation of probes was observed for β and α in the mixtures of IL and dipolar aprotic co-solvents, whereas the hydrogen-bond interactions between IL and dipolar protic co-solvent enabled the preferential solvation to be alleviated and resulted in more linear variation of β and α against the molar fraction of IL. The results not only contribute to a better understanding of the effect of co-solvent on imidazolium-based chloride ILs, but also are instructive for improving the thermodynamic performance of IL-based applications via providing IL+co-solvent mixtures with desirable physicochemical properties

Massive particles' Hawking radiation via tunneling from the G.H Dilaton black hole

In the past, Hawking radiation was viewed as a tunneling process and the barrier was just created by the outgoing particle itself. In this paper, Parikh's recent work is extended to the case of massive particles' tunneling. We investigate the behavior of the tunneling massive particles from a particular black hole solution-G.H Dilaton black hole which is obtained from the string theory, and calculate the emission rate at which massive particles tunnel across the event horizon. We obtain that the result is also consistent with an underlying unitary theory. Furthermore, the result takes the same functional form as that of massless particles.Comment: 6 pages, no figure, revtex

Massive uncharged and charged particles' tunneling from the Horowitz-Strominger Dilaton black hole

Originally, Parikh and Wilczek's work is only suitable for the massless particles' tunneling. But their work has been further extended to the cases of massive uncharged and charged particles' tunneling recently. In this paper, as a particular black hole solution, we apply this extended method to reconsider the tunneling effect of the H.S Dilaton black hole. We investigate the behavior of both massive uncharged and charged particles, and respectively calculate the emission rate at the event horizon. Our result shows that their emission rates are also consistent with the unitary theory. Moreover, comparing with the case of massless particles' tunneling, we find that this conclusion is independent of the kind of particles. And it is probably caused by the underlying relationship between this method and the laws of black hole thermodynamics.Comment: 6 pages, no figure, revtex 4, accepted by Int. J. Mod. Phys

A New Digital Watermarking Algorithm Based On DWT-DCT-RLC

Abstract: The paper presents an imperceptible and robust digital watermarking algorithm using a combination of the DWT-DCT , which improves the robustness via using new embedding strategy. Apply 4-level DWT to the host image. Then, DCT transform of each directional middle frequencies DWT subband is computed; A binary watermarked logo is coded by Run-Length Code(RLC);The watermark bits are embedded in the coefficients of the DCT block based on the energy of each corresponding to coefficient in the highest level selected using the value of RLC. Performance evaluation show that the proposed scheme is invisible and robust against common image manipulations like JPEG compression, noise adding, sharpening, filtering. I Introduction Image watermarking is the process of embedding the ownership information into host data so that intellectual copyrights can be identified. Along with the rapid growth of novel watermarking schemes, various attacking attempts have also been developed to destroy watermarking II Theory A.Run Length Code Run-length code (RLC) is a very simple form of data compression in which runs of data (that is, sequences in which the same data value occurs in many consecutive data elements) are stored as a single data value and count, rather than as the original run. This is most useful on data that contains many such runs: for example, simple graphic images such as icons, line drawings, and animations

Emotional Mechanisms in Supervisor-Student Relationship: Evidence from Machine Learning and Investigation

How to cultivate innovative talents has become an important educational issue nowadays. In China’s long-term mentorship education environment, supervisor-student relationship often affects students’ creativity. From the perspective of students’ psychology, we explore the influence mechanism of supervisor-student relationship on creativity by machine learning and questionnaire survey. In Study 1, based on video interviews with 16 postgraduate students, we use the machine learning method to analyze the emotional states exhibited by the postgraduate students in the videos when associating them with the supervisor-student interaction scenario, finding that students have negative emotions in bad supervisor-student relationship. Subsequently, we further explore the impact of supervisor-student relationship on postgraduate students’ development in supervisor-student interaction scenarios at the affective level. In Study 2, a questionnaire survey is conducted to explore the relationship between relevant variables, finding that a good supervisor-student relationship can significantly reduce power stereotype threat, decrease emotional labor surface behaviors, and promote creativity expression. The above results theoretically reveal the internal psychological processes by which supervisor-student relationship affects creativity, and have important implications for reducing emotional labor and enhancing creativity expression of postgraduate students