16S forestland soil raw sequence reads

The aim of his project is to determine the impact of cultivating wine-cap Stropharia (Stropharia rugosoannulata) on the soil nutrients and bcterial community composition of forestland, at a depth: 0-30 cm. Soil samples were taken in China (Jining) after wine-cap Stropharia harvest in October 2016. Following the DNA extraction, the DNA was sequenced using IlluminaHiSeq2500 platform, and 250 bp paired-end reads were generated

sj-pdf-1-ajs-10.1177_03635465231194028 – Supplemental material for Meniscal Fibrocartilage Repair Based on Developmental Characteristics

Supplemental material, sj-pdf-1-ajs-10.1177_03635465231194028 for Meniscal Fibrocartilage Repair Based on Developmental Characteristics by Wenqiang Yan, Jingxian Zhu, Yue Wu, Yiqun Wang, Cancan Du, Jin Cheng, Xiaoqing Hu and Yingfang Ao in The American Journal of Sports Medicine</p

Detection of ADAMTS‑4 Activity Using a Fluorogenic Peptide-Conjugated Au Nanoparticle Probe in Human Knee Synovial Fluid

A disintegrin and metalloproteinase with thrombospondin motif-4 (ADAMTS-4) plays a pivotal role in degrading aggrecan, which is an early event in cartilage degrading joint diseases such as osteoarthritis (OA). Detection of ADAMTS-4 activity could provide useful clinical information for early diagnosis of such diseases and disease-modifying therapy. Therefore, we developed a ADAMTS-4 detective fluorescent turn-on AuNP probe (ADAMTS-4-D-Au probe) by conjugating gold nanoparticles with a FITC-modified ADAMTS-4-specific peptide (DVQEFRGVTAVIR). When the ADAMTS-4-D-Au probe was incubated with ADAMTS-4, the fluorescence recovered and fluorescence intensity markedly increased in proportion to concentrations of ADAMTS-4 and the probe. A nearly 3-fold increase in fluorescent intensity in response to only 3.9 pM of ADAMTS-4 was detected, whereas almost no fluorescence recovery was observed when the probe was incubated with matrix metalloproteinase (MMP)-1, -3, and -13. These results indicate a relative high sensitivity and specificity of the probe. Moreover, ADAMTS-4-D-Au probe was used to detect ADAMTS-4 activity in synovial fluid from 11 knee surgery patients. A substantial increase in fluorescent intensity was observed in the acute joint injury group as compared to the chronic joint injury and end-stage OA groups, indicating that this simple and low-cost sensing system might serve as a new detection method for ADAMTS-4 activity in biological samples and in screens for inhibitors for ADAMTS-4-related joint diseases. Additionally, this probe could be a potential biomarker for early diagnosis of cartilage-degrading joint diseases