Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer

Colorectal cancer is one of the malignant tumors with the highest mortality rate in the world. Survival rates vary significantly among patients at various stages of the disease. A biomarker capable of early diagnosis is required to facilitate the early detection and treatment of colorectal cancer. Human endogenous retroviruses (HERVs) are abnormally expressed in various diseases, including cancer, and have been involved in cancer development. Real-time quantitative PCR was used to detect the transcript levels of HERV-K(HML-2) gag, pol, and env in colorectal cancer to systematically investigate the connection between HERV-K(HML-2) and colorectal cancer. The results showed that HERV-K(HML-2) transcript expression was significantly higher than healthy controls and was consistent at the population and cell levels. We also used next-generation sequencing to identify and characterize HERV-K(HML-2) loci that were differentially expressed between colorectal cancer patients and healthy individuals. The analysis revealed that these loci were concentrated in immune response signaling pathways, implying that HERV-K impacts the tumor-associated immune response. Our results indicated that HERV-K might serve as a screening tumor marker and a target for tumor immunotherapy in colorectal cancer

Synthesis and characterization of coordination compounds of Cd(II), Co(II), Ni(II), Cu(II) and Zn(II) with rutin

2010-2014The synthesis, characterization and electrochemical properties of five novel coordination compounds of rutin with transition metal [M = Cd(II), Co(II), Ni(II), Cu(II) and Zn(II)] are reported here. These coordination compounds may be formulated as [M3L2(H2O)n] (CH3COO)2 (n=6,12). Three hydroxy groups on the unsaturated rings form coordination bonds with the metal ions. The cyclic voltammetric data show changes in the redox potentials and currents of the coordination compounds relative to that of rutin. Formation of the coordination compounds changes the rigidity of rutin molecular plane and has a little influence on the antioxidation of rutin

Voltammetric response of 2,4-D-molecularly imprinted film modified glassy carbon electrodes

1848-1851A novel voltammetric sensor has been developed by changing medium based on the molecular recognition of the template by imprinted technology. The sensitive layer has been prepared by electropolymerization of ortho-phenylenediamine and resorcinol on glassy carbon electrode in the presence of the template molecule. Cyclic voltammetry and a.c. impedance studies have been carried out to investigate the properties of the polymeric film. After the template extraction from the resultant solution, rebinding of 2,4-D has been done from 0.1 mol/L H2SO4 containing the analyte. The amount of bonded 2,4-D has been determined by differential pulse voltammetry. The sensor exhibits good sensitivity and reproducibility for 2,4-D by virtue of the interaction between molecularly imprinted binding sites and template

Synthesis of a series of triaza-macrocyclicanes

372-375Condensation of the N,N',N"-tris(p-toluenesulfonyl)diethylenetrimine-N,N"-disodium with bissulphonate esters of two-, three- four- and six-carbon diols gives the corresponding 9-, 10-,11-, 13-membered triamine macrocycles containing N-p-toluenesulfonyl group, respectively. The p-tuluenesulfonyl groups are removed with concentrated sulfuric acid, acetic acid hydrobromic acid or LiAlH4 which give the ammonium salts of 1,4,7-triazacyclononane, 1,4,7-triazacyclodecane, 1,4,7-triazacycloundecane. 1,4,7-triazacyclotridecane, respectively. Comparison and details of the different synthetic procedures of the triamine macrocycles are described as well