Enhancing Higher Order Question of Student Through Problem Based Learning at Grade X MIA 6 of SMA N 4 Surakarta

The research aims to enhance the Higher Order Question of student through problem based learning in Biology at Grade X MIA 6 of SMA N 4 Surakarta. The research was a four-cycle action research conducted in academic year 2014/2015. All questions were analyzed based on revised Bloom Taxonomy. Data were validated using triangulation method. The result of the research showed that problem based learning effectively enhance student\u27s High Order Question (C4-C6). The percentage of each High Order Question (C4-C6) in pre cycle were 0%. The percentage of C4 type question at first cycle (73,14%), second cycle (52,13%), third cycle (56,05%), and fourth cycle (58,42%). The percentage of each High Order Question (C4-C6) in pre cycle were 0%. The percentage of C5 type question at first cycle (18,37%), second cycle (9,57%), third cycle (10,30%), and fourth cycle (58,42%). The percentage of each High Order Question (C4-C6) in pre cycle were 0%. The percentage of C6 type question at first cycle (8,16%), second cycle (38,30%), third cycle (41,18%) and fourth cycle (25,74%)

Knowledge and attitudes about newborn screening for Fabry disease

Newborn screening is a public health program that identifies newborns who are at risk of having a life-threatening condition that will affect their health in infancy or childhood. Fabry disease is an X-linked lysosomal storage disorder with a variable age of onset from childhood through adulthood that was recently added to a few states’ newborn screening panels. Research on patient attitudes towards newborn screening for Fabry disease has been limited and this qualitative study aimed to gain a more complete understanding of the reasoning of adults with Fabry disease regarding the appropriateness of newborn screening for Fabry disease, their knowledge of newborn screening, and their experiences with Fabry disease. Participants were recruited from Children’s Hospital of Pittsburgh of UPMC’s Lysosomal Storage Disorders Clinic and six adults who have Fabry disease were interviewed. These interviews were transcribed and thematic analysis revealed six themes: influences of clinical spectrum and severity of Fabry disease, support systems, family dynamics, impact of timing of diagnosis and treatment availability on attitudes towards newborn screening, knowledge and attitudes towards newborn screening for Fabry disease, and impact of earlier diagnosis. Based on their personal experiences with Fabry disease, all participants were in favor of newborn screening for Fabry disease. Participants’ experiences with Fabry disease also reflected aspects of their family dynamics. The results of this qualitative study can inform genetic counseling practice for Fabry disease and future studies on NBS for Fabry disease. The opinions of stakeholders, including patients affected by the condition, are of public health significance and the results of this study can inform public health decisions as state legislators and state newborn screening programs consider whether to include Fabry disease on their state’s newborn screening panel

Effects of probiotics consumption on lowering lipids and CVD risk factors: A systematic review and meta-analysis of randomized controlled trials

<div><p>This meta-analysis examined the effect of probiotics on the reduction of lipid components and coexisting risk factors associated with cardiovascular disease. All randomized controlled trials published in English on PubMed and Scopus from 2000 to 2014 were systematically searched. Using the PEDro scale to assess the quality of studies, a total of 15 studies with 788 subjects were selected for inclusion in the analysis. The mean difference and effect size with a 95% confidence interval (CI) were extracted from individual studies. Statistically significant pooled effects of probiotics were found on reduction of total cholesterol, low-density lipoprotein (LDL), body mass index (BMI), waist circumference, and inflammatory markers. Subgroup analysis revealed statistically significant effects of probiotics on total cholesterol and LDL when the medium was fermented milk or yogurt (<i>P</i> < 0.001) compared to capsule form, consumption was at least 8 weeks in duration (<i>P</i> < 0.001), and the probiotics consisted of multiple strains (<i>P</i> < 0.001) rather than a single strain. A significant reduction was found in LDL in trials which contained <i>Lactobacillus Acidophilus</i> strain (<i>P</i> < 0.001) compared to other types of strains. Our findings suggest that probiotic supplementation use is effective in lowering the lipid level and coexisting factors associated with cardiovascular disease.</p></div

Mesalazine Modified-Release Tablet in the Treatment of Ulcerative Colitis in the Active Phase: A Chinese, Multicenter, Single-Blind, Randomized Controlled Study

<p><b>Article full text</b></p><p><br></p><p>The full text of this article can be found <a href="https://link.springer.com/article/10.1007/s12325-016-0303-z"><b>here</b>.</a></p><p><br></p><p><b>Provide enhanced content for this article</b></p><p><br></p><p>If you are an author of this publication and would like to provide additional enhanced content for your article then please contact <a href="http://www.medengine.com/Redeem/”mailto:[email protected]”"><b>[email protected]</b></a>.</p><p> </p><p>The journal offers a range of additional features designed to increase visibility and readership. All features will be thoroughly peer reviewed to ensure the content is of the highest scientific standard and all features are marked as ‘peer reviewed’ to ensure readers are aware that the content has been reviewed to the same level as the articles they are being presented alongside. Moreover, all sponsorship and disclosure information is included to provide complete transparency and adherence to good publication practices. This ensures that however the content is reached the reader has a full understanding of its origin. No fees are charged for hosting additional open access content.</p><p><br></p><p>Other enhanced features include, but are not limited to:</p><p><br></p><p>• Slide decks</p><p>• Videos and animations</p><p>• Audio abstracts</p><p> • Audio slides<br></p

How much could be saved in Chinese hospitals in procurement of anti-hypertensives and anti-diabetics?

<p><b>Background:</b> Efficient use of government funding has been increasingly relevant for the success and sustainability of ongoing health-system reform in China; however, as there is no generic substitution policy, patients and basic health-insurance programs pay more for public-preferred brand originators. Such phenomenon is especially typical in public hospitals. The objective of this study is to estimate the potential cost savings in procurement by Chinese public hospitals when switching from brand originators of anti-hypertensive and anti-diabetic medications to their generic equivalents between 2012–2014.</p> <p><b>Method:</b> IMS Health volume and value consumption data (IMS China Hospitals Audit system 2012–2014) were used, which covered all Chinese hospitals with 100 beds and above. The top 60% IMS volume consumption of respective anti-hypertensive and anti-diabetic medication with unique dosage form and strength were included. The potential cost savings were calculated from a switch of brand originators with their generic equivalents on the Chinese and international market. An independent sample <i>t</i>-test was conducted to compare the difference of proportion of cost savings in value between the Chinese and international market.</p> <p><b>Results:</b> An average of 44% (US$44 million) and 87$90 million) and a total of US$1.4 and 2.8 billion (2014 US$) could be saved from a switch from originator brand anti-hypertensives and anti-diabetics to domestically and internationally available generic equivalents, respectively. The differences of cost savings (in proportion) between domestic and international market were statistically significant (<i>α</i> = 0.005, <i>p</i> = 0.003, <i>p</i> = 0.002, <i>p</i> = 0.000).</p> <p><b>Conclusion:</b> Expensive brand originators dominated the anti-hypertensive and anti-diabetic market in Chinese hospitals between 2012–2014. Preference of brand originators wastes a huge amount of health resources in China and these limited resources could have been used more efficiently. As one of the world’s key generic suppliers, if China wants to use its health resource more efficiently on medicines, comprehensive measures are needed to address both demand-side (consumers’ low trust in the quality of local generics) and supply-side barriers (health professionals’ preference of brand originators).</p

Construction of Dispirocyclopentanebisoxindoles via Self-Domino Michael-Aldol Reactions of 3‑Phenacylideneoxindoles

A simple protocol for the construction of novel dispirocyclopentanebisoxindoles is accomplished by the base promoted domino reactions between two molecules of 3-phenacylideneoxindoles with the participation of solvents, alcohol, or other added nucleophiles such as amines or thiophenols. Significantly, this domino reaction results in the complex dispiro compounds with high yields and diastereoselectivity, which would allow construction of dispirocyclopentanebisoxindole with four and five diastereoisomeric centers using simple materials

The effectiveness of multi-dimensional resilience rehabilitation programs after traumatic physical injuries: a systematic review and meta-analysis

<p><b>Objective:</b> To synthesize evidence of the effectiveness of socio-ecological resilience rehabilitation programs on returning to work (RTW), self-efficacy, and stress mitigation following traumatic physical injuries.</p> <p><b>Methods:</b> PubMed, Scopus, Proquest, Cinahl, Web of Science, Clinical Trials Database, and the Cochrane Central Register of Controlled Trials databases were searched. Methodological quality was assessed using the PEDro tool.</p> <p><b>Study selection:</b> Randomized interventions aimed at promoting resilience.</p> <p><b>Data extraction:</b> Twenty one studies were reviewed (11,904 participants). Data from 19 studies of high methodological quality were pooled using a random-effects meta-analysis. Mean differences for continuous outcomes and risk ratios for binary outcomes were calculated.</p> <p><b>Data synthesis:</b> Resilience rehabilitation programs significantly increased the likelihood of ever RTW (OR 2.09, 95% CI 0.99–4.44, <i>p</i> = 0.05), decreased the number of days taken to return to work (mean difference –7.80, 95% CI –13.16 to –2.45, <i>p</i> ≤ 0.001), and increased total self-efficacy scores (mean difference 5.19, 95% CI 3.12–7.26, <i>p</i> < 0.001). Subgroup analyses found that favorable return to work outcomes resulted from programs involving workplace support (<i>p</i> < 0.001) and for people with musculoskeletal or orthopedic injuries (<i>p</i> = 0.02).</p> <p><b>Conclusions:</b> Compared to rehabilitation programs providing standard care following injuries, programs aimed at developing resilience could improve reemployment outcomes and self-efficacy.Implications for rehabilitation</p><p>Individual resilience may be an important factor promoting functional recovery after traumatic injury.</p><p>Resilience rehabilitation programs are effective in enabling patients’ return to work and increasing their self efficacy. In particular, programs involving the workplace are important components for enabling optimal work participation outcomes.</p><p></p> <p>Individual resilience may be an important factor promoting functional recovery after traumatic injury.</p> <p>Resilience rehabilitation programs are effective in enabling patients’ return to work and increasing their self efficacy. In particular, programs involving the workplace are important components for enabling optimal work participation outcomes.</p

Image_1_Identification of molecular subtypes and immune infiltration in endometriosis: a novel bioinformatics analysis and In vitro validation.tif

IntroductionEndometriosis is a worldwide gynacological diseases, affecting in 6–10% of women of reproductive age. The aim of this study was to investigate the gene network and potential signatures of immune infiltration in endometriosis.MethodsThe expression profiles of GSE51981, GSE6364, and GSE7305 were obtained from the Gene Expression Omnibus (GEO) database. Core modules and central genes related to immune characteristics were identified using a weighted gene coexpression network analysis. Bioinformatics analysis was performed to identify central genes in immune infiltration. Protein-protein interaction (PPI) network was used to identify the hub genes. We then constructed subtypes of endometriosis samples and calculated their correlation with hub genes. qRTPCR and Western blotting were used to verify our findings.ResultsWe identified 10 candidate hub genes (GZMB, PRF1, KIR2DL1, KIR2DL3, KIR3DL1, KIR2DL4, FGB, IGFBP1, RBP4, and PROK1) that were significantly correlated with immune infiltration. Our study established a detailed immune network and systematically elucidated the molecular mechanism underlying endometriosis from the aspect of immune infiltration.DiscussionOur study provides comprehensive insights into the immunology involved in endometriosis and might contribute to the development of immunotherapy for endometriosis. Furthermore, our study sheds light on the underlying molecular mechanism of endometriosis and might help improve the diagnosis and treatment of this condition.</p

Table_5_Identification of molecular subtypes and immune infiltration in endometriosis: a novel bioinformatics analysis and In vitro validation.docx

IntroductionEndometriosis is a worldwide gynacological diseases, affecting in 6–10% of women of reproductive age. The aim of this study was to investigate the gene network and potential signatures of immune infiltration in endometriosis.MethodsThe expression profiles of GSE51981, GSE6364, and GSE7305 were obtained from the Gene Expression Omnibus (GEO) database. Core modules and central genes related to immune characteristics were identified using a weighted gene coexpression network analysis. Bioinformatics analysis was performed to identify central genes in immune infiltration. Protein-protein interaction (PPI) network was used to identify the hub genes. We then constructed subtypes of endometriosis samples and calculated their correlation with hub genes. qRTPCR and Western blotting were used to verify our findings.ResultsWe identified 10 candidate hub genes (GZMB, PRF1, KIR2DL1, KIR2DL3, KIR3DL1, KIR2DL4, FGB, IGFBP1, RBP4, and PROK1) that were significantly correlated with immune infiltration. Our study established a detailed immune network and systematically elucidated the molecular mechanism underlying endometriosis from the aspect of immune infiltration.DiscussionOur study provides comprehensive insights into the immunology involved in endometriosis and might contribute to the development of immunotherapy for endometriosis. Furthermore, our study sheds light on the underlying molecular mechanism of endometriosis and might help improve the diagnosis and treatment of this condition.</p

Image_3_Identification of molecular subtypes and immune infiltration in endometriosis: a novel bioinformatics analysis and In vitro validation.tif

IntroductionEndometriosis is a worldwide gynacological diseases, affecting in 6–10% of women of reproductive age. The aim of this study was to investigate the gene network and potential signatures of immune infiltration in endometriosis.MethodsThe expression profiles of GSE51981, GSE6364, and GSE7305 were obtained from the Gene Expression Omnibus (GEO) database. Core modules and central genes related to immune characteristics were identified using a weighted gene coexpression network analysis. Bioinformatics analysis was performed to identify central genes in immune infiltration. Protein-protein interaction (PPI) network was used to identify the hub genes. We then constructed subtypes of endometriosis samples and calculated their correlation with hub genes. qRTPCR and Western blotting were used to verify our findings.ResultsWe identified 10 candidate hub genes (GZMB, PRF1, KIR2DL1, KIR2DL3, KIR3DL1, KIR2DL4, FGB, IGFBP1, RBP4, and PROK1) that were significantly correlated with immune infiltration. Our study established a detailed immune network and systematically elucidated the molecular mechanism underlying endometriosis from the aspect of immune infiltration.DiscussionOur study provides comprehensive insights into the immunology involved in endometriosis and might contribute to the development of immunotherapy for endometriosis. Furthermore, our study sheds light on the underlying molecular mechanism of endometriosis and might help improve the diagnosis and treatment of this condition.</p