6 research outputs found
Compartmentalized Assembly of Motor Protein Reconstituted on Protocell Membrane toward Highly Efficient Photophosphorylation
Molecule assembly
and functionalization of protocells have achieved
a great success. However, the yield efficiency of photophosphorylation
in the present cell-like systems is limited. Herein, inspired by natural
photobacteria, we construct a protocell membrane reconstituting motor
protein for highly efficient light-mediated adenosine triphosphate
(ATP) synthesis through a layer-by-layer technique. The assembled
membrane, compartmentally integrating photoacid generator, proton
conductor, and ATP synthase, possesses excellent transparency, fast
proton production, and quick proton transportation. Remarkably, these
favorable features permit the formation of a large proton gradient
in a confined region to drive ATP synthase to produce ATP with high
efficiency (873 ATP s<sup>–1</sup>). It is the highest among
the existing artificial photophosphorylation systems. Such a biomimetic
system provides a bioenergy-supplying scenario for early photosynthetic
life and holds promise in remotely controlled ATP-consumed biosensors,
biocatalysts, and biodevices
Transporting a Tube in a Tube
LbL-assembled
tubes were employed for micro/nanoscale cargo transportation
through the kinesin-microtubule system. Selectively modified with
kinesins onto the inner tube walls through Ni–NTA complexes,
these tubes can work as channels for microtubules. A motility assay
shows the smooth movement of microtubules along the tube inner wall
powered by the inside immobilized kinesins. It could be envisioned
that cargoes with different sizes can be transported through these
tubular channels with little outside interruption
Hypocrellin-Loaded Gold Nanocages with High Two-Photon Efficiency for Photothermal/Photodynamic Cancer Therapy <i>in Vitro</i>
A new bioconjugate nanostructure was constructed by using photosensitizer-incorporated mixed lipid-coated gold nanocages for two-photon photothermal/photodynamic cancer therapy <i>in vitro</i> with high efficiency. Scanning electron microscopic and transmission electron microscopic images reveal that the precursors and bioconjugate nanostructure as-prepared are narrowly dispersed and possess uniform morphologies. The relevant energy dispersion X-ray analysis and UV–vis spectra indicate that the bioconjugate nanostructure above was assembled successfully and has a strong absorption in the near-infrared region. Fluorescence and electronic spin resonance results show that the gold nanocage in the bioconjugate nanostructure can dramatically quench the photosensitizer and inhibit the production of singlet oxygen, which is supposed to alleviate the photosensitizers’ unwanted side effects originating from their nontargeted distribution. We have demonstrated that as the nanocomplex is internalized by cancer cells, under two-photon illumination, photodynamic anticancer treatment is dramatically enhanced by the photothermal effect
One-Pot Ultrafast Self-Assembly of Autofluorescent Polyphenol-Based Core@Shell Nanostructures and Their Selective Antibacterial Applications
We demonstrate that large-scale autofluorescent tea polyphenol (TP)-based core@shell nanostructures can be assembled by one-pot preparation under microwave irradiation within 1 min. The formation mechanism of the heterogeneous well-defined core@shell nanocomposites involves microwave-assisted oxidation-inducing self-assembly and directed aggregation. The strategy is general to construct Ag@TP and Au@TP nanocomposites. Moreover, a simple galvanic replacement reaction was introduced to synthesize hollow Au/Ag@TP bioconjugates with near-infrared (NIR) absorption, which could be exploited for NIR cancer diagnosis and treatment. It could be expected that more complex alloy@TP nanostructures can be obtained under proper reaction conditions. Furthermore, as a first application, it is shown that the heterogeneous Ag@TP nanostructures can strongly inhibit Escherichia coli growth, while they exhibit no obvious normal cell toxicity. The sharp contrast of the two effects promises that the nanocomposites are excellent low toxicity biomaterials for selective antibacterial treatment
Fabrication of Mesoporous Silica Nanoparticle with Well-Defined Multicompartment Structure as Efficient Drug Carrier for Cancer Therapy <i>in Vitro</i> and <i>in Vivo</i>
Vaterite particles are composed of
particulate CaCO<sub>3</sub> nanoparticles, which offer an ideal platform
to synthesize architectures
with hierarchical structure. Herein we show that mesoporous silica
particles with well-defined multicompartment structure are fabricated
by employing vaterite particles as templates. The obtained silica
particles inherited the structure feature of vaterite and had excellent
biocompatibility and biodegradability. Moreover, the silica particles
were established as an efficient anticancer drugs carrier compared
with hollow silica particles, which could be applied in cancer therapy <i>in vitro</i> and <i>in vivo</i>. The silica particles
obtained here offer a cheap, facile, environmentally friendly avenue
to assembly of hierarchical drugs carriers
Self-Assembly of Ultralong Aligned Dipeptide Single Crystals
Oriented arrangement
of single crystals plays a key role in improving
the performance of their functional devices. Herein we describe a
method for the exceptionally fast fabrication (mm/min) of ultralong
aligned dipeptide single crystals (several centimeters). It combines
an induced nucleation step with a continuous withdrawal of substrate,
leading to specific evaporation/composition conditions at a three-phase
contact line, which makes the growth process controllable. These aligned
dipeptide fibers possess a uniform cross section with active optical
waveguiding properties that can be used as waveguiding materials.
The approach provides guidance for the controlled arrangement of organic
single crystals, a family of materials with considerable potential
applications in large-scale functional devices