The Leighton Chajnantor Telescope: Project update and mechanical structural analysis in preparations for new deployment in Chajnantor, Chile

The LCT project aims to refurbish the CSO telescope, move it from Maunakea to Chajnantor, in Chile, and operate it scientifically for 10 years. The relocation of the telescope involves a variety of changes in the working conditions, which demands in-depth mechanical analysis. To conduct the required studies, an FEM model of the entire telescope has been developed, together with CFD tools. This paper introduces the LCT project, presents the full-FEM model, its validation, and the first steps towards these analyses. Preliminary results of the simulations of the telescope, considering the working conditions at the Plateau, are also shown

Undoped Strained Ge Quantum Well with Ultrahigh Mobility Grown by Reduce Pressure Chemical Vapor Deposition

We fabricate an undoped Ge quantum well under 30 nm Ge0.8Si0.2 shallow barrier with reverse grading technology. The under barrier is deposited by Ge0.8Si0.2 followed by Ge0.9Si0.1 so that the variation of Ge content forms a sharp interface which can suppress the threading dislocation density penetrating into undoped Ge quantum well. And the Ge0.8Si0.2 barrier introduces enough in-plane parallel strain -0.41% in the Ge quantum well. The heterostructure field-effect transistors with a shallow buried channel get a high two-dimensional hole gas (2DHG) mobility over 2E6 cm2/Vs at a low percolation density of 2.51 E-11 cm2. We also discover a tunable fractional quantum Hall effect at high densities and high magnetic fields. This approach defines strained germanium as providing the material basis for tuning the spin-orbit coupling strength for fast and coherent quantum computation.Comment: 11 pages, 5 figure

Dietary Corn Bran Altered the Diversity of Microbial Communities and Cytokine Production in Weaned Pigs

Corn bran (CB) has been used as an ingredient for pigs, but the underlying mechanisms that improve gut health is less clear. This study was conducted to investigate effects of dietary CB on growth performance, nutrient digestibility, plasma indices related to gut hormones and immunity, gut microbiota composition, and fermentation products in weaned pigs. A total of 60 weaned pigs were allocated to two dietary treatments, and piglets in each group received control (CON) diet or 5% CB diet for 28 days. Growth performance, nutrient digestibility, indices of gut hormones and immunity in plasma were evaluated. Microbiota composition in feces was determined using 16S rRNA amplicon sequencing, and fermentation products were measured by high-performance ion chromatography. The results showed that dietary CB did not affect growth performance, nutrient digestibility, gut hormones, or fermentation products in the trial (P > 0.05). There was an increased response to CB inclusion on interleukin-10 production (P < 0.05). On day 28, piglets fed dietary CB had a higher shannon index (P < 0.05). The population of the Firmicutes in CB treatment were decreased (P < 0.05), while the percentage of the Bacteroidetes were increased (P < 0.05). In particular, the populations of Eubacterium corprostanoligenes, Pevotella, and Fibrobacter related to polysaccharide fermentation of cereal bran were increased (P < 0.05). In conclusion, a post-weaning diet containing 5% CB increased intestinal microbial diversity, especially higher richness of fibrolytic bacteria, and promoted anti-inflammatory response to some extent in piglets, these changes should facilitate the adaptation of the digestive system of piglets in the subsequent growing phases

Combination of Clostridium butyricum and Corn Bran Optimized Intestinal Microbial Fermentation Using a Weaned Pig Model

Experimental manipulation of the intestinal microbiota influences health of the host and is a common application for synbiotics. Here Clostridium butyricum (C. butyricum, C.B) combined with corn bran (C.B + Bran) was taken as the synbiotics application in a waned pig model to investigate its regulation of intestinal health over 28 days postweaning. Growth performance, fecal short chain fatty acids (SCFAs) and bacterial community were evaluated at day 14 and day 28 of the trial. Although the C.B + Bran treatment has no significant effects on growth performance (P > 0.05), it optimized the composition of intestinal bacteria, mainly represented by increased acetate-producing bacteria and decreased pathogens. Microbial fermentation in the intestine showed a shift from low acetate and isovalerate production on day 14 to enhanced acetate production on day 28 in the C.B + Bran treatment. Thus, C.B and corn bran promoted intestinal microbial fermentation and optimized the microbial community for pigs at an early age. These findings provide perspectives on the advantages of synbiotics as a new approach for effective utilization of corn barn

Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate

IntroductionLiver cancers exhibit abnormal (leaky) vasculature, hypoxia and an immunosuppressive microenvironment. Normalization of tumor vasculature is an emerging approach to treat many cancers. Blockmir CD5-2 is a novel oligonucleotide-based inhibitor of the miR-27a interaction with VE-Cadherin, the endothelial-specific cadherin. The combination of a vasoactive medication with inhibition of immune checkpoints such as programmed cell death protein 1 (PD1) has been shown to be effective in treating liver cancer in humans. We aimed to study the effect of CD5-2 combined with checkpoint inhibition (using an antibody against PD1) on liver tumor growth, vasculature and immune infiltrate in the diethylnitrosamine (DEN)-induced liver tumor mouse model.MethodsWe first analyzed human miR-27a and VE-Cadherin expression data from The Cancer Genome Atlas for hepatocellular carcinoma. CD5-2 and/or anti-PD1 antibody were given to the DEN-treated mice from age 7-months until harvest at age 9-months. Tumor and non-tumor liver tissues were analyzed using histology, immunohistochemistry, immunofluorescence and scanning electron microscopy.ResultsHuman data showed high miR-27a and low VE-Cadherin were both significantly associated with poorer prognosis. Mice treated with CD5-2 plus anti-PD1 antibody had significantly smaller liver tumors (50% reduction) compared to mice treated with either agent alone, controls, or untreated mice. There was no difference in tumor number. Histologically, tumors in CD5-2-treated mice had less leaky vessels with higher VE-Cadherin expression and less tumor hypoxia compared to non-CD5-2-treated mice. Only tumors in the combination CD5-2 plus anti-PD1 antibody group exhibited a more favorable immune infiltrate (significantly higher CD3+ and CD8+ T cells and lower Ly6G+ neutrophils) compared to tumors from other groups.DiscussionCD5-2 normalized tumor vasculature and reduced hypoxia in DEN-induced liver tumors. CD5-2 plus anti-PD1 antibody reduced liver tumor size possibly by altering the immune infiltrate to a more immunosupportive one

Sodium Decanoate Improves Intestinal Epithelial Barrier and Antioxidation via Activating G Protein-Coupled Receptor-43

The study was conducted to explore actions of decanoic acid on regulating intestinal barrier and antioxidant functions in intestinal epithelium cells isolated from porcine jejunum (IPEC-J2) and C57/BL6 mice models. In vitro and vivo assays, mice and IPEC-J2 cells treated by H2O2 were disposed of sodium decanoate and sodium butyrate to determine intestinal barrier and antioxidant functions of the host. Results showed that sodium decanoate upregulated expression of tight junction proteins and improved antioxidant capacity in both IPEC-J2 cells treated by H2O2 and mice models (p < 0.05). Sodium decanoate increased weight gain and ileal villus height of mice compared with control and sodium butyrate treatments (p < 0.05). Sodium decanoate increased α-diversity of ileal microbiota, volatile fatty acids concentration, and G protein-coupled receptor-43 (GPR-43) expression in the ileum and colon of mice (p < 0.05). In conclusion, sodium decanoate improved antioxidant capacity, intestinal morphology, and gut physical barrier of intestinal epithelial cells, resulting in an increase growth performance of mice, which is mediated through activating GPR-43 signaling

Effects of Extrusion on Energy Contents and Amino Acid Digestibility of Corn DDGS and Full-Fat Rice Bran in Growing Pigs

The objectives of this study were to determine the effects of extrusion on available energy, apparent total tract digestibility (ATTD) of nutrients and energy, and amino acid (AA) digestibility of full-fat rice bran (FFRB) and corn distillers dried grain with solubles (DDGS) fed to growing pigs. Methods: In Exp. 1, a total of 30 growing pigs with initial body weight (BW) of 36.0 ± 1.8 kg were fed five different diets, including one corn basal diet and four experimental diets which were formulated by 29.06% FFRB or DDGS with or without extrusion processing. In Exp. 2, 30 ileal-cannulated pigs (initial BW: 20.3 ± 1.8 kg) were fed five different diets including 40% FFRB or DDGS with or without extrusion, and a N-free diet. Results: The results showed that there were no significant differences in DE and ME contents or ATTD of GE, DM, and OM between DDGS and FFRB (p > 0.05), but the ATTD of CP, NDF, and ADF showed significant differences between DDGS and FFRB (p p p p = 0.06 and 0.07, respectively). The AID and SID levels of CP were not different when pigs were fed diets with or without extrusion. The AID of total indispensable AA increased when pigs were fed extrusion diets (p < 0.05). Conclusion: Feed processing of extrusion could improve nutritive values of FFRB and DDGS

Digestible energy and metabolizable energy contents of konjac flour residues and ramie in growing pigs

The objectives of this study were to determine: 1) the effects of konjac flour residues and ramie on digestible energy (DE), metabolizable energy (ME) and apparent total tract digestibility (ATTD) of nutrients in diets fed to growing pigs, 2) the DE and ME contents of konjac flour residues and ramie. Thirty barrows were allotted to 1 of 5 treatments with 6 replicates per treatment. The 5 diets include a corn-soybean meal basal diet (CTL), konjac flour residues diets containing 15% konjac flour residues (LK) or 30% konjac flour residues (HK), and ramie diets containing 15% ramie (LR) or 30% ramie (HR). The experiment lasted 19 days, including 7 days for cage adaptation, 7 days for diet adaptation, and 5 days for total feces and urine collection. The energy values and ATTD of nutrients in each diet were determined, and DE and ME contents of konjac flour residues and ramie were calculated. The results showed that consumption of konjac flour residues significantly increased (P < 0.01) the fecal moisture content compared with the ramie treatment. The LK, HK and HR diets had lower (P < 0.01) DE values compared with the CTL diet. The HR diet had greater (P < 0.01) DE value compared with the HK diet. The LK and LR diets showed greater (P < 0.01) ATTD of DM, OM, GE and CP compared with the HK and HR diets. The HK diet had the lowest (P < 0.01) ATTD of ether extract (EE) among the 5 diets. No differences were observed for the ATTD of NDF and ADF among the 5 diets. Moreover, the DE and ME values of konjac flour residues under 2 inclusion levels (15% and 30%) were 11.66, 11.87 MJ/kg and 10.41, 10.03 MJ/kg, respectively. The corresponding values for ramie were 13.27, 13.16 MJ/kg and 13.07, 12.82 MJ/kg, respectively. In conclusion, the differences in fecal moisture content and the ATTD of EE among the 5 diets were mainly due to the different chemical compositions of konjac flour residues and ramie. Compared with konjac flour residues, ramie has greater DE and ME values under the same inclusion level. Keywords: Apparent total tract digestibility, Digestible energy, Konjac flour residues, Metabolizable energy, Rami

Fructooligosaccharide Reduces Weanling Pig Diarrhea in Conjunction with Improving Intestinal Antioxidase Activity and Tight Junction Protein Expression

This study was to illustrate the effects of fructooligosaccharide (FOS) on the antioxidant capacity, intestinal barrier function, and microbial community of weanling pigs. Results showed that FOS reduced the incidence of diarrhea (6.5 vs. 10.8%) of pigs (p p p p p p < 0.05). In conclusion, FOS activated Nrf2 signaling and increased the expression of specific tight junction proteins, which were associated with reduced diarrhea incidence

Biological Function of Antimicrobial Peptides on Suppressing Pathogens and Improving Host Immunity

The emergence of drug-resistant genes and concerns about food safety caused by the overuse of antibiotics are becoming increasingly prominent. There is an urgent need for effective alternatives to antibiotics in the fields of livestock production and human medicine. Antimicrobial peptides can effectively replace antibiotics to kill pathogens and enhance the immune functions of the host, and pathogens cannot easily produce genes that are resistant to them. The ability of antimicrobial peptides (AMPs) to kill pathogens is associated with their structure and physicochemical properties, such as their conformation, electrical charges, hydrophilicity, and hydrophobicity. AMPs regulate the activity of immunological cells and stimulate the secretion of inflammatory cytokines via the activation of the NF-κB and MAPK signaling pathways. However, there are still some limitations to the application of AMPs in the fields of livestock production and human medicine, including a restricted source base, high costs of purification and expression, and the instability of the intestines of animals and humans. This review summarizes the information on AMPs as effective antibiotic substitutes to improve the immunological functions of the host through suppressing pathogens and regulating inflammatory responses. Potential challenges for the commercial application of AMPs in animal husbandry and human medicine are discussed