The present status and prospect of research on the de-ironing and bleaching technology of kaolin

The increase of degree of whiteness is an important method for Kaolin growth in value. In this paper, the occurrence of iron in kaolin was briefly described, and the methods of the de-ironing and bleaching technology of kaolin were introduced. The characteristics and the existing problems of all kinds of method were analyzed. Some new green environmental protection technology of the de-ironing and bleaching kaolin were introduced

BENEFIT AUDIT OF R&D INVESTMENT MANAGEMENT

Long time before , it is mostly blank about study of Benefit Audit Of R&D Investment Management for R&D incertitude of active in my country. This article be discuss by benefit audit of R&D investment management form three aspect, namely it is about testing basic Assumptions 、measuring the cost of nonconformance and analyzing the complete colligate of R&D investment management .That is study for change of lag estate of R&D investment management in my country almost corporation. Key words: R&D, Investment Management, Benefit, Audi

Optimization of polysaccharides extracted from Verbena officinalis L and their inhibitory effects on invasion and metastasis of colorectal cancer cells

Purpose: To investigate polysaccharides (PEV) extracted from the aerial part of Verbena officinalis L. and their inhibitory effects on the invasion and metastasis of colorectal cancer (CRC) cells.Methods: PEV was extracted by water and the optimization of extraction conditions was performed using a Box-Benhnken design (BBD). The cell viability was evaluated by 4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay. The effects of PEV on cell adhesion and invasion were evaluated by Transwell invasion, wound healing migration and adhesion assays in vitro. The effects of PEV on the expressions of matrix metalloproteinase-9 (MMP-9), mesenchymal-epithelial transition factor (C-met), E-cadherin (E-CAD), cyclooxygenase-2 (COX-2) and E-prostanoid 2 receptor (EP2) were investigated by western blot.Results: The BBD model was established successfully for the optimization of PEV extraction (p <0.0001). The results indicate that PEV (50, 100 and 200 μg/mL) had significant inhibitory effects on cell invasion and migration of SW480 cells (p < 0.05). PEV also significantly decreased cell adhesion of SW480 cells. By treating with PEV, the expressions of C-met, MMP-9, COX-2 and EP2 were decreased, whereas the expression of E-CAD increased in SW480 cells.Conclusion: RSM is effective for optimizing the extraction conditions of PEV. More importantly, PEV significantly inhibits the invasion and metastasis of SW480 cells by regulating the expression of MMP-9, C-met, E-CAD, COX-2 and EP2. Thus, PEV has the potential to be developed into therapeutic drugs for CRC in the future.Keywords: Polysaccharides, Colorectal cancer, Verbena officinalis, SW480 cell lines, Cell invasion, Metastasi