1 research outputs found
Synthetic α- and β‑Ser-ADP-ribosylated Peptides Reveal α‑Ser-ADPr as the Native Epimer
A solid-phase methodology to synthesize
oligopeptides, specifically
incorporating serine residues linked to ADP-ribose (ADPr), is presented.
Through the synthesis of both α- and β-anomers of the
phosphoribosylated Fmoc-Ser building block and their usage in our
modified solid-phase peptide synthesis protocol, both α- and
β-ADPr peptides from a naturally Ser-ADPr containing H2B sequence
were obtained. With these, and by digestion studies using the human
glycohydrolase, ARH3 (hARH3), compelling evidence is obtained that
the α-Ser-ADPr linkage comprises the naturally occurring configuration