Chloride intracellular channel 3: a secreted pro-invasive oxidoreductase

No abstract available

EXAFS Analysis of Size-Constrained Semiconducting Materials

Semiconducting materials such as CdSe, CdS, PbS and GaP are included in crystalline zeolite Y and mordenite and structurally flexible ethylene-methacrylic acid copolymer solid matrices. EXAFS analysis reveals formation of species with dimensions of molecular size up to ca. 13 A in the crystalline hosts, while the polymer matrices allow agglomeration of larger semiconducting particles. Zeolite anchored structures are distinctively different to small particles with bulk crystal structure as usually found in colloidal systems

Response of river-dominated delta channel networks to permanent changes in river discharge

Using numerical experiments, we investigate how river-dominated delta channel networks are likely to respond to changes in river discharge predicted to occur over the next century as a result of environmental change. Our results show for a change in discharge up to 60% of the initial value, a decrease results in distributary abandonment in the delta, whereas an increase does not significantly affect the network. However, an increase in discharge beyond a threshold of 60% results in channel creation and an increase in the density of the distributary network. This behavior is predicted by an analysis of an individual bifurcation subject to asymmetric water surface slopes in the bifurcate arms. Given that discharge in most river basins will change by less than 50% in the next century, our results suggest that deltas in areas of increased drought will be more likely to experience significant rearrangement of the delta channel network. Copyright 2010 by the American Geophysical Union

αvβ3 and α5β1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration

Accumulating evidence suggests that integrin recycling regulates cell migration. However, the lack of reagents to selectively target the trafficking of individual heterodimers, as opposed to endocytic transport as a whole, has made it difficult to define the contribution made by particular recycling pathways to directional cell movement. We show that autophosphorylation of protein kinase D1 (PKD1) at Ser916 is necessary for its association with αvβ3 integrin. Expression of PKD1916A or the use of mutants of β3 that do not bind to PKD1 selectively inhibits short-loop, Rab4-dependent recycling of αvβ3, and this suppresses the persistence of fibroblast migration. However, we report that short-loop recycling does not directly contribute to fibroblast migration by moving αvβ3 to the cell front, but by antagonizing α5β1 recycling, which, in turn, influences the cell's decision to migrate with persistence or to move randomly

Tensin links energy metabolism to extracellular matrix assembly.

The regulation of integrin function is key to fundamental cellular processes, including cell migration and extracellular matrix (ECM) assembly. In this issue, Georgiadou et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201609066) report that the metabolic sensor adenosine monophosphate-activated protein kinase influences tensin production to regulate α5β1-integrin and fibrillar adhesion assembly and thus reveal an important connection between energy metabolism and ECM assembly

Quarried: Three decades of Pine Mountain Sand & Gravel Literary Journal

A project of the Southern Appalachian Writers Cooperative, Pine Mountain Sand & Gravel has given voice to a wide range of Appalachian writers since the mid-1980s. It has no institutional support, but rather is a grassroots effort of writers, editors and activists from the region. Quarried is collection of poetry, fiction and nonfiction spanning more than thirty years of literature from Appalachia\u27s 13-state sprawl. It is edited by Weatherford award-winner Richard Hague and includes writing by Jim Wayne Miller, Lee Howard, Bob Snyder, Silas House, George Ella Lyon, Chris Holbrook, Ed McClanahan and Jane Hicks, among many other well-known and emerging Appalachian writers from across the region. Current and past editors of the journal (Pauletta Hansel, moderator; Richard Hague, Scott Goebel, Gurney Norman and Jim Webb) will be joined by contributors for a lively and enlivening reading and conversation about the history and future of this literary journal with grit

A proteomic approach to identify endosomal cargoes controlling cancer invasiveness

We have previously shown that Rab17 - a small GTPase associated with epithelial polarity - is specifically suppressed by ERK2 signalling to promote an invasive phenotype. However, the mechanisms through which Rab17 loss permits invasiveness, and the endosomal cargoes that are responsible for mediating this are not known. Using quantitative mass spectrometry-based proteomics, we have found that knockdown of Rab17 leads to highly selective reduction in the cellular levels of a v-SNARE (Vamp8). Moreover, proteomics and immunofluorescence indicate that Vamp-8 is associated with Rab17 at late endosomes. Reduced levels of Vamp8 promote transition between ductal carcinoma in situ (DCIS) and a more invasive phenotype. We developed an unbiased proteomic approach to elucidate the complement of receptors that redistributes between endosomes and the plasma membrane, and have pin-pointed neuropilin-2 (NRP2) as a key pro-invasive cargo of Rab17/Vamp8-regulated trafficking. Indeed, reduced Rab17 or Vamp8 levels lead to increased mobilisation of NRP2-containing late endosomes and upregulated cell surface expression of NRP2. Finally, we show that NRP2 is required for the basement membrane disruption which accompanies transition between DCIS and a more invasive phenotype