SIN001, drug for improvement of the embryonic implantation. Study of endometrial markers in clinical regulatory phase

Motivation:The study of endometrial receptivity has opened a new approach in the world of assisted reproduction, allowing the development of genetic tests, drugs and techniques that increase the rate of “child at home” mainly in couples who are attending to assisted reproduction programmes. Genetic tests about endometrial receptivity allow to evaluate the “window of implantation” (WOI) estimating the best moment for successful embryo transfer, decreasing the maternal factor. Due to the progress of these tests it can be considered if drugs like SIN001, improve endometrial receptivity in clinical phase. Methods:Through the endometrial biopsy before and after the intake of the drug SIN001 of 32 women in a natural cycle, the transcriptome is analysed, after extracting RNA, using quantitative PCR in microfluidics technique (Fluidigm technology). It consists of a dynamic array where the samples and the primers of the genes, in this case 192 markers previously described and related to the endometrial receptivity, are combined in a real-time PCR. A pre-treatment and treatment histological determination can be obtained due to the endometrial biopsy, for the expression and localization of some of the proteins that have been defined as important in endometrial receptivity.Results: In the preclinical in vitro tests obtained with the drug SIN001, the toxicity was dismissed and the adhesion increase was observed in cell culture models and in primary cultures models of endometrial epithelium. In this clinical phase is expected to improve the WOI in women after the intake of the drug and therefore the embryo implantation. In addition, to evaluate the receptive status of the women with the endometrial receptivity test and also it is expected to establish the expression profile of the 192 genes in the transcriptomics, as well as an evaluation of the proteins obtained in immunohistochemistry.Conclusions: Currently there are no drugs improving embryo implantation, this means that the discovery of a substance like SIN001, significantly increase the probability of success in reproduction clinics, since the drug is expected to cover three phases: (i) endometrial preparation; (ii) embryo implantation; (iii) placentation and prevention of spontaneous miscarriage. Therefore, the next steps to scale up the drug to market would be to increase the cohort of the study and studies focusing on the action mechanism of the drug SIN001 in endometrium

Natural parasitism of the Citrus Leafminer (Lepidoptera: Gracillariidae) over eight years in seven citrus regions of São Paulo, Brazil

The citrus leafminer (CLM) Phyllocnists citrella Stainton (Lepidoptera: Gracillariidae) was frst recorded in Brazil in 1996. In 1998, the parasitoid Ageniaspis citricola Logvinovskaya (Hymenoptera: Encyrtdae) was introduced and established in many regions of the country. In this study, 130 onehour-samplings of sweet orange leaves (Citrus sinensis [L.] Osbeck) hostng CLM pupal chambers were carried out to estmate the CLM parasitsm rate (%) by its parasitoids in 7 regions of São Paulo State between 2000 and 2008. The sample sizes varied from 10 to 275 leaves (mean = 65). The most abundant parasitoid was the encyrtd A. citricola (found in 91.8% of the samplings). The highest level of CLM parasitsm by A. citricola was recorded in the southern region (Botucatu), 70.2 ± 6.6 (mean ± SEM), and the lowest level was recorded in the northern region (Barretos), 12.8 ± 5.7%. CLM parasitsm by A. citricola and by natve parasitoids (Galeopsomyia fausta LaSalle, Cirrospilus spp. and Elasmus sp.) did not differ between seasons. The 6-fold increase in the use of insectcides in citrus groves, afer 2004 when the Huanglongbing (HLB) disease was found in São Paulo State, did not reduce the level of CLM parasitsm. The level of parasitsm was 50.8 ± 4.2% before the advent of HLB (2000–2004) and 56.0 ± 4.4% thereafer (2005–2008), indicatng adaptaton of A. citricola in a disturbed agroecosystem.A minadora das folhas dos citros (MFC), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), foi encontrada pela primeira vez no Brasil em 1996. Em 1998, o parasitoide Ageniaspis citricola Logvinovskaya (Hymenoptera: Encyrtidae) foi introduzido e se estabeleceu em várias regiões do país. Nesse estudo, foram feitas130 amostragens, de uma hora, de folhas de laranjeiras doces [Citrus sinensis (L.) Osbeck] com câmaras pupais da MFC, para se estimar o parasitismo da MFC em 7 regiões do estado de São Paulo, entre 2000 e 2008. O tamanho das amostras variou de 10 a 275 folhas (média = 65). O parasitoide mais abundante foi o encirtídeo A. citricola (encontrado em 91.8% das amostragens). O maior parasitismo da MFC por A. citricola foi observado na região sul do estado (Botucatu), 70,2 ± 6,6 (média ± EPM), e o menor parasitismo na região norte (Barretos), 12,8 ± 5,7%. O parasitismo da MFC por A. citricola e seus parasitoides nativos (Galeopsomyia fausta LaSalle, Cirrospilus spp. and Elasmus sp.) não diferiram entre as estações do ano. O aumento de seis vezes no uso de inseticidas nos pomares de citros, após 2004, quando o Huanglongbing (HLB) foi encontrado no estado de São Paulo, não reduziu o nível de parasitismo da MFC. O nível médio de parasitismo foi de 50,8 ± 4,2%, antes do HLB (2000-2004), e 56,0 ± 4,4%, após o HLB (2005-2008), indicando a adaptação de A. citricola a um agroecossistema perturbado.info:eu-repo/semantics/publishedVersio

Situación actual de los metadatos en el ámbito internacional

En un prinicipio, los metadatos han sido considerados como atributos descriptivos de las principales caracterísiticas de los recursos relacionados con la información ..

Linezolid: idoneidad de prescripción y afectación de la función renal y hematológica

The objective of the study was to evaluate the suitability of linezolid prescription, incidence of thrombocytopenia incritically ill patients and its infl uence on critical ill patients with renal disorder before the linezolid treatment. Weobserved that the 37.5% of linezolid prescriptions were in Non-Approved Indications in Spain (NAIS). The incidenceof thrombocytopenia was 5.9%. We observed a signifi cant improvement in renal function of patients after two weeks oflinezolid treatment (p=0.024). The incidence of thrombocytopenia was similar to describe in bibliography. It seems tobe necessary approved news indications in Spain.El objeto de este estudio fue evaluar la idoneidad de prescripción de linezolid y si ésta se ajusta a las indicacionesaprobadas en España (IAE), la incidencia de trombocitopenia en pacientes críticos y la infl uencia de linezolid eneste tipo de pacientes con función renal alterada previamente a la instauración del tratamiento con dicho antibiótico.Se recogieron retrospectivamente datos clínicos y analíticos, observando que el 37.5% de las prescripcionesde linezolid eran en indicaciones no aprobadas en España (INAE), una incidencia de trombocitopenia del 5.9% yuna mejora signifi cativa de la función renal (p=0.024) tras dos semanas de tratamiento con linezolid. La incidenciade trombocitopenia en nuestro estudio fue similar a la descrita en la bibliografía. Parece necesario replantearse laampliación de las INAE

Las IDE como evolución natural de los SIG

La IDEE es un proyecto cooperativo, de autoría colectiva, en el que colaboran organismos e instituciones de los tres ámbitos de la Administración (general, regional y local), del entorno universitario y del sector privado. Esta impresionante oferta de información geográfica, junto con las funcionalidades que aportan las tecnologías de Infraestructuras de Datos Espaciales (IDE), permite vislumbrar un abanico de líneas de trabajo, todavía inexploradas, de gran interés para todos los especialistas, técnicos e investigadores que manejan o precisan de cartografía en su quehacer cotidiano, que veremos en el presente artículo

Los clubes deportivos de natación de Cataluña en el actual contexto competitivo del sector de las instalaciones deportivas

Swimming clubs are non-profit entities for sports and social purposes. The general objective of the study was to know the management of swimming clubs in Catalonia, fulfilling their social and sports role in the current competitive environment of sports facilities. As specific objectives were to know their values on management indicators, as well as to know if significant differences between swimming clubs are found, taking in consideration their areas and sports offer. The study sample consists of 19 swimming clubs of Catalonia. The data collection instrument was a questionnaire adapted to the objectives of the study, administered by email to the managers of each club. The clubs obtain the necessary income to achieve their sporting and social goals thanks to the fees of their members. It was found significant differences between them according to the spaces and sports offer in different management indicators analyzed.Los clubes de natación son entidades sin ánimo de lucro con fines deportivos y sociales. El objetivo general del estudio fue conocer la gestión de los clubes de natación en Cataluña cumpliendo su función social y deportiva en el actual entorno competitivo de instalaciones deportivas. Los objetivos específicos fueron conocer sus valores en indicadores de gestión, así como saber si existen diferencias significativas entre los clubes según sus espacios y oferta deportiva. La muestra del estudio ha sido formada por 19 clubes deportivos de natación de Cataluña. El instrumento de recogida de datos fue un cuestionario adaptado a los objetivos del estudio, administrado por correo electrónico a los gerentes de cada club. Los clubes obtienen los ingresos necesarios para lograr sus fines deportivos y sociales gracias a las cuotas de sus socios. Se encontraron diferencias significativas entre ellos según los espacios y oferta deportiva en diferentes indicadores de gestión analizados

Understanding patient-reported knowledge of hernia surgery: a quantitative study

Abdominal wall; Knowledge; PatientPared abdominal; Conocimiento; PacienteParet abdominal; Coneixement; PacientPurpose The objective of this study was to gather information on patient-reported knowledge (PRK) in the field of hernia surgery. Methods A prospective quantitative study was designed to explore different aspects of PRK and opinions regarding hernia surgery. Patients referred for the first time to a surgical service with a presumed diagnosis of hernia and eventual hernia repair were eligible, and those who gave consent completed a simple self-assessment questionnaire before the clinical visit. Results The study population included 449 patients (72.8% men, mean age 61.5). Twenty (4.5%) patients did not have hernia on physical examination. The patient’s perceived health status was “neither bad nor good” or “good” in 56.6% of cases. Also, more patients considered that hernia repair would be an easy procedure (35.1%) rather than a difficult one (9.8%). Although patients were referred by their family physicians, 32 (7.1%) answered negatively to the question of coming to the visit to assess the presence of a hernia. The most important reason of the medical visit was to receive medical advice (77.7%), to be operated on as soon as possible (40.1%) or to be included in the surgical waiting list (35.9%). Also, 46.1% of the patients considered that they should undergo a hernia repair and 56.8% that surgery will be a definitive solution. Conclusion PRK of patients referred for the first time to an abdominal wall surgery unit with a presumed diagnosis of hernia was quite limited and there is still a long way towards improving knowledge of hernia surgery.Open Access Funding provided by Universitat Autonoma de Barcelona

Una nueva etapa: hacia la IDE 2.0

El desarrollo de las Infraestructuras de Datos Espaciales (IDE) en España ha cubierto una primera etapa basada en el despliegue de servicios básicos, aplicaciones de visualización y apertura de geoportales. Una IDE paradigmática de esta primera fase, que podemos llamar convencionalmente IDE 1.0, incluiría: un visualizador con servicios de mapas WMS de ortofotos, imágenes de satélite y cartografía, un catálogo de metadatos (CSW, SRW, otro perfil, o soluciones no estándar), un servicio de Nomenclátor (WFS-G, WFS-MNE o soluciones no estándar) para realizar búsquedas por nombre, un servicio de descarga de datos (basado en WFS), ,y probablemente aplicaciones complementarias no estándar al margen de las specificaciones OGC, como, por ejemplo, utilidades de transformación de coordenadas, o un cliente pesado para realizar vuelos virtuales. En suma, la mayoría de los geoportales disponibles están orientados fundamentalmente a la visualización de datos geográficos