Differences in erythrocyte sodium transport between human plasma and artificial medium:the role and character of sodium efflux and influx stimulating plasma factors

The main objective of this study was to further characterize the plasma factor(s) which stimulate sodium efflux from erythrocytes, which we reported previously. Dialysis of plasma against an artificial medium using membranes with varying molecular mass cut-off points revealed relative molecular mass(es) of the factor(s) of 1 00- 1 000 Da. The factor(s) could be absorbed on Dowex at pH 1. 5 and Amberlite at pH 11.0, indicating 'Zwitterionic' character. They are hydrophilic and resistant to acid hydrolysis. These characteristics and direct measurements of contents made amino acids likely candidates for the efflux stimulating properties of the factor(s). Indeed, plasma amino acids added to artificial medium could abolish the sodium efflux difference between plasma and the artificial medium. The efflux stimulating effect of amino acids appeared not to be the result of sodium influx stimulation. A coincident finding was that plasma also contains dialyzable sodium influx stimulating factor(s) which are not amino acids

Development of an assessment methodology to evaluate agricultural use of plant protection products for drinking water production from surface waters : a proposal for the registration procedure in the Netherlands

Two tiers were developed to assess the drinking water standard of 0.1 μg/L at nine locations where surface water is abstracted to produce drinking water in the Netherlands. Two tiers were developed to assess the drinking water standard of 0.1 μg/L at nine locations where surface water is abstracted to produce drinking water in the Netherlands. In Tier I, concentrations at the abstraction points are calculated on the basis of edge-of field surface water concentrations for all crops in the intake area on which the pesticide can be used. The edge-of-field concentrations are corrected to estimate the concentration at the abstraction point by factors accounting for e.g. the relative cropped area of the intake area, degradation and difference in timing of application

Development of an assessment methodology to evaluate agricultural use of plant protection products for drinking water production from surface waters : a proposal for the registration procedure in the Netherlands

Two tiers were developed to assess the drinking water standard of 0.1 μg/L at nine locations where surface water is abstracted to produce drinking water in the Netherlands. Two tiers were developed to assess the drinking water standard of 0.1 μg/L at nine locations where surface water is abstracted to produce drinking water in the Netherlands. In Tier I, concentrations at the abstraction points are calculated on the basis of edge-of field surface water concentrations for all crops in the intake area on which the pesticide can be used. The edge-of-field concentrations are corrected to estimate the concentration at the abstraction point by factors accounting for e.g. the relative cropped area of the intake area, degradation and difference in timing of application

Whole genome sequencing resource identifies 18 new candidate genes for autism spectrum disorder

We are performing whole-genome sequencing of families with autism spectrum disorder (ASD) to build a resource (MSSNG) for subcategorizing the phenotypes and underlying genetic factors involved. Here we report sequencing of 5,205 samples from families with ASD, accompanied by clinical information, creating a database accessible on a cloud platform and through a controlled-access internet portal. We found an average of 73.8 de novo single nucleotide variants and 12.6 de novo insertions and deletions or copy number variations per ASD subject. We identified 18 new candidate ASD-risk genes and found that participants bearing mutations in susceptibility genes had significantly lower adaptive ability (P = 6 × 10-4). In 294 of 2,620 (11.2%) of ASD cases, a molecular basis could be determined and 7.2% of these carried copy number variations and/or chromosomal abnormalities, emphasizing the importance of detecting all forms of genetic variation as diagnostic and therapeutic targets in ASD