Biological cell image segmentation using novel hybrid morphology-based method

Conference Name:2012 5th International Symposium on Computational Intelligence and Design, ISCID 2012. Conference Address: Hangzhou, China. Time:October 28, 2012 - October 29, 2012.In this paper, we propose a novel hybrid method for the segmentation and automatic counting of biological cell image. The method is based on techniques of morphology, thresholding and watershed. It performs well in low contrast image where gradient-based method may fail. Experimental results on practical cell images are shown in the paper with the emphasis on the comparisons between the novel hybrid method and the gradient-based methods: Sobel [1], Canny [2] and GAC [3] of level-set. 漏 2012 IEEE

Multilayer Physical Model of Eyeball with Application to Simulation of IOP Increase

Conference Name:International Symposium on Computer Science and Computational Technology. Conference Address: Shanghai, PEOPLES R CHINA. Time:DEC 20-22, 2008.It's very significative to get the numerical prediction of the biomechanical response of eyeball to increased IOP. In this study, a geometry model of eyeball with 3-layers wall and optic nerve was built firstly; then, meshing and physical property was applied on it to get physical model; finally, the increase of IOP was simulated on it. The results of simulation experiment validate this model and the feasibility of this method. Based on this, more complex and individual model of human eyeball can be built

Ultrasound-assisted extraction of polyphenols from pine needles (Pinus elliottii): Comprehensive insights from RSM optimization, antioxidant activity, UHPLC-Q-Exactive Orbitrap MS/MS analysis and kinetic model

Extracting polyphenolic bioactive compounds from Pinus elliottii needles, a forestry residue, promises economic and environmental benefits, however, relevant experimental data are lacking. Herein, a comprehensive investigation of the polyphenolic composition of pine needles (PNs) was carried out. Ultrasound-Assisted Extraction (UAE) was applied to extract the polyphenolic compounds of pine needles. The optimal conditions of extracts were determined by Response Surface Methodology (RSM). The maximum total phenolic content (TPC) of 40.37 mg GAE/g PNs was achieved with solid–liquid ratio of 1:20, 60 % ethanol, and 350 W for 25 min at 45 °C. Polyphenolic extracts showed antioxidant activity in scavenging free radicals and reducing power (DPPH, IC50 41.05 μg/mL; FRAP 1.09 mM Fe2+/g PNs; ABTS, IC50 214.07 μg/mL). Furthermore, the second-order kinetic model was also constructed to describe the mechanism of the UAE process, with the extraction activation energy estimated at 12.26 kJ/mol. In addition, 37 compounds in PNs were first identified by UHPLC-Q-Exactive Orbitrap MS/MS, including flavonoids and phenolic acids. The results suggest that Ultrasound-Assisted is an effective method for the extraction of natural polyphenolic compounds from pine needles and this study could serve as a foundation for utilizing phenolics derived from PNs in the food and pharmaceutical industries

High-Precision In Situ Total Alkalinity Analyzer Capable of Month-Long Observations in Seawaters

Total alkalinity (TA) is an essential variable for the study of physical and biogeochemical processes in coastal and oceanic systems, and TA data obtained at high spatiotemporal resolutions are highly desired. The performance of the current in situ TA analyzers/sensors, including precision, accuracy, and deployment duration, cannot fully meet most research requirements. Here, we report on a novel high-precision in situ analyzer for surface seawater TA (ISA-TA), based on an automated single-point titration with spectrophotometric pH detection, and capable of long-term field observations. The titration was carried out in a circulating loop, where the titrant (a mixture of HCl and bromocresol green) and seawater sample were mixed in a constant volume ratio. The effect of ambient temperature on the TA measurement was corrected with an empirical formula. The weight, height, diameter, and power consumption of ISA-TA were 8.6 kg (in air), 33 cm, 20 cm, and 7.3 W, respectively. A single measurement required ∼7 min of running time, ∼32 mL of seawater, and ∼0.6 mL of titrant. ISA-TA was able to operate continuously in the field for up to 30 days, and its accuracies in the laboratory and field were 0.5 ± 1.7 μmol kg–1 (n = 13) and 10.3 ± 2.8 μmol kg–1 (n = 29) with precisions of 0.6–0.8 μmol kg–1 (n = 51) and 0.2–0.7 μmol kg–1 (n = 8), respectively. This study provides the research community with a new tool to obtain seawater TA data of high temporal resolution