Research on the Mechanism of Entrepreneurship Education on College Students’ Entrepreneurial Willingness and Its Future Prediction

The strength of college students’ entrepreneurial willingness is a barometer for measuring the effectiveness of entrepreneurship education. It is also an important avenue for college students to expand their employment opportunities and enhance the quality of their employment in the face of new employment trends. Comprehensive universities offer a wide range of disciplines and great professional specialization. It is of great significance to explore the diversity results in college students’ entrepreneurship education indicators. According to the data on the relationship between entrepreneurial education and entrepreneurship willingness in comprehensive universities in Jiangsu province, various factors such as subject characteristics, work experience, educational background, and family environment significantly impact college students’ willingness to become entrepreneurs. The implementation of entrepreneurship education, including the awakening of entrepreneurial consciousness, the cultivation of entrepreneurial abilities, and the improvement of entrepreneurial willingness, has a direct impact on college students’ willingness to start their own businesses

Cytomegalovirus Infection May Trigger Adult-Onset Still's Disease Onset or Relapses

Previous studies have revealed that several micro-organisms, especially DNA viruses, have been associated with adult-onset Still's disease (AOSD). However, there are no studies on the relationship between the presence of viral infections in AOSD patients with disease occurrence and reactivation. In the present study, we aimed to investigate the presence of antibodies against virus, virus DNA load and nucleic acid sensors in AOSD patients. Anti-viral antibodies were measured by enzyme-linked immunosorbent assay (ELISA) in plasma samples from 100 AOSD patients and 70 healthy controls (HCs). The copy number of cytomegalovirus (CMV) DNA in 100 AOSD patients was detected by PCR. The expression levels of nucleic acid sensors interferon gamma-inducible protein 16 (IFI16) and absent in melanoma 2 (AIM2) in peripheral blood mononuclear cell (PBMC) and skin from AOSD patients and HCs were analyzed by PCR and immunohistochemistry. The levels of antibodies against CMV were significantly higher in AOSD patients compared to HCs. Moreover, the level of anti-CMV IgM antibody was significantly increased in patients with fever, sore throat, arthralgia and rash. CMV DNA was found in plasma of AOSD patients with disease new-onset and relapse. Furthermore, the copy number of CMV DNA significantly increased in patients with fever, sore throat, arthralgia and rash. And the significant associations of the CMV DNA level with the levels of leukocytes, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were observed. Moreover, we found an upregulation of cytoplasmic DNA-sensing receptor IFI16 and AIM2 in PBMC and skin from AOSD patients. In conclusion, our results showed that CMV infection may play a role in the initiation or amplification of inflammatory responses in AOSD

Plasma microRNA Profiles as a Potential Biomarker in Differentiating Adult-Onset Still's Disease From Sepsis

Adult-onset Still's disease (AOSD) is a systemic inflammatory disease characterized by cytokine storm. However, a diagnostic test for AOSD in clinical use is yet to be validated. The aim of our study was to identify non-invasive biomarkers with high specificity and sensitivity to diagnosis of AOSD. MicroRNA (miRNA) profiles in PBMC from new-onset AOSD patients without any treatment and healthy controls (HCs) were analyzed by miRNA deep sequencing. Plasma samples from 100 AOSD patients and 60 HCs were used to validated the expression levels of miRNA by qRT-PCR. The correlations between expression levels of miRNAs and clinical manifestations were analyzed using advanced statistical models. We found that plasma samples from AOSD patients showed a distinct miRNA expression profile. Five miRNAs (miR-142-5p, miR-101-3p, miR-29a-3p, miR-29c-3p, and miR-141-3p) were significantly upregulated in plasma of AOSD patients compared with HCs both in training and validation sets. We discovered a panel including 3 miRNAs (miR-142-5p, miR-101-3p, and miR-29a-3p) that can predict the probability of AOSD with an area under the receiver operating characteristic (ROC) curve of 0.8250 in training and validation sets. Moreover, the expression levels of 5 miRNAs were significantly higher in active AOSD patients compared with those in inactive patients. In addition, elevated level of miR-101-3p was found in AOSD patients with fever, sore throat and arthralgia symptoms; the miR-101-3p was also positively correlated with the levels of IL-6 and TNF-α in serum. Furthermore, five miRNAs (miR-142-5p, miR-101-3p, miR-29c-3p, miR-29a-3p, and miR-141-3p) expressed in plasma were significantly higher in AOSD patients than in sepsis patients (P < 0.05). The AUC value of 4-miRNA panel (miR-142-5p, miR-101-3p, miR-29c-3p, and miR-141-3p) for AOSD diagnosis from sepsis was 0.8448, revealing the potentially diagnostic value to distinguish AOSD patients from sepsis patients. Our results have identified a specific plasma miRNA signature that may serve as a potential non-invasive biomarker for diagnosis of AOSD and monitoring disease activity

Dyadic Green’s Function and the Application of Two-Layer Model

Dyadic Green’s function (DGF) is a powerful and elegant way of solving electromagnetic problems in the multilayered media. In this paper, we introduce the electric and magnetic DGFs in free space, respectively. Furthermore, the symmetry of different kinds of DGFs is proved. This paper focuses on the application of DGF in a two-layer model. By introducing the universal form of the vector wave functions in space rectangular, cylindrical and spherical coordinates, the corresponding DGFs are obtained. We derive the concise and explicit formulas for the electric fields represented by a vertical electric dipole source. It is expected that the proposed DGF can be extended to some more electric field problem in the two-layer model

Theoretical Logic and Implementation Path of Comprehensive Land Consolidation for Promoting Common Prosperity: A Case Study of Ningbo City

Eliminating poverty and achieving social justice are global concerns. China has focused on common prosperity. Comprehensive land consolidation is a potential policy tool that can contribute to common prosperity, but its effectiveness and implementation methods are yet to be verified and discussed. Therefore, we construct an analytical framework to understand how comprehensive land consolidation promotes common prosperity. The pilot area for comprehensive land consolidation in Ningbo City is used as a qualitative case study. The research results indicate that comprehensive land consolidation focuses on development and sharing to promote high-quality socio-economic development, social fairness, and justice. The paths for achieving development included the following: (1) a network governance structure consisting of multiple entities to enhance land governance; (2) various consolidation activities were conducted at the town scale to optimize the functionality and spatial pattern of public spaces; and (3) enabling the flow of urban and rural factors for improved resource allocation efficiency and providing an impetus for industrial development. The paths for achieving sharing included clarifying collective land ownership and promoting land transactions to provide diverse land values and ensure a shared distribution. This research provides new insights applicable to other Chinese cities and numerous developing countries engaged in land consolidation to address social distribution issues

Branch-Selective Addition of Unactivated Olefins into Imines and Aldehydes

Radical hydrofunctionalization occurs with ease using met-al-hydride atom transfer (MHAT) catalysis to couple alkenes and competent radicalophilic electrophiles. Traditional two-electron electrophiles have remained unreactive. Herein we report the addition of electronically-unbiased olefins into imines and aldehydes. Iron-catalysis allows addition of alkyl-substituted olefins into imines through the intermediacy of free-radicals, whereas a combination of catalytic Co(Salt-Bu,t-Bu) and chromium salts enable a branch-selective coupling of olefins and aldehydes through the formation of a putative alkyl chromium intermediate

Structurally Dynamic Gelatin-Based Hydrogels with Self-Healing, Shape Memory, and Cytocompatible Properties for 4D Printing

Three-dimensional (3D) printable hydrogels with a shape memory effect have emerged as a new class of 4D printing materials recently and found wide applications in various fields. However, synergistically endowing such materials with good mechanical strength and biocompatibility for biomedical uses remains challenging. In this study, a series of multiresponsive hydrogels have been prepared through a dynamic covalent imine/Diels–Alder network from biocompatible starting materials of modified gelatin and poly(ethylene glycol)-based polymers. By further secondary crosslinking with a hyperbranched triethoxysilane reagent (HPASi) that contains multiple supramolecular hydrogen bonding, the hydrogels presented a strengthened self-healing and temperature-responsive shape memory effect. With the additional features of superior stretchability (elongation at break up to 523%), good cytocompatibility, and 3D printable properties, these multifunctional hydrogels showed great potential for broad biomedical applications

The D ~ Sense ex-vivo viability assay application in a patient with stage IV lung adenocarcinoma: a case report

Abstract Background The treatment resistance is a problem for lung cancer. In this study, we used a vitro tissue culturing system to select a new therapy strategy for a patient with tyrosine kinase inhibitors (TKIs) resistance. Case presentation A 42-year-old male Asian patient was diagnosed with advanced lung adenocarcinoma harboring an exon 19 deletion in the epidermal growth factor receptor (EGFR) gene. The patient was treated with Gefitinib, resulting in an almost complete remission for over a year. The patient relapsed after 13 months treatment, and received four cycles of chemotherapy. At 20 months, the patient had developed multiple lung metastases and a solitary cerebellar metastasis. An EGFR T790M mutation was identified in the peripheral blood sample. Subsequent treatment with Osimertinib resulted in a complete response of the intracranial metastasis. By 33 months, the patient had developed a mediastinal tumor mass that responded well to local radiotherapy. By 39 months, an EGFR C797S cis-mutation had been identified and the patient was treated with Brigatinib and Cetuximab. By 44 months, the tumor cells from the pleural effusion had been tested for sensitivity against 30 targeted and cytostatic drugs using the D ~ Sense ex-vivo viability assay. The assay identified 8 drugs with moderate to high sensitivity. Combination therapy of Gemcitabin and Lobaplatin had resulted in disease stabilization. Conclusions The case showed that individualized treatment aided by D ~ Sense ex-vivo viability assay can be a viable option for patients with advanced lung adenocarcinoma with pleural effusions

Convergent Total Synthesis of (+)-Calcipotriol: A Scalable, Modular Approach to Vitamin D Analogs

Vitamin D is a group of seco-steroids with diverse bioactivities. An enormous amount of effort was expended by medicinal chemists to search for Vitamin D analogs that could exhibit pro-differentiating and antiproliferative effects on normal and cancer cells as well as immunomodulatory effects without causing hypercalcemia. A convergent approach for the total synthesis of calcipotriol (brand name: Dovonex), a proven Vitamin D analog used for the treatment of psoriasis, and medicinally relevant synthetic analogs is described. Given the rich synthetic history of the Vitamin D family, a complete novel approach towards both the A-ring and CD-ring is reported. From a retrosynthetic standpoint, hidden symmetry within the decorated A-ring is disclosed, which allowed for scalable quantities of this advanced intermediate. In addition, a radical retrosynthetic approach is described, which highlights an electrochemical reductive coupling as well as an intramolecular hydrogen atom transfer (HAT)-Giese addition to establish the 6,5-trans-carbon skeleton found in the Vitamin D family. Lastly, a late-stage decarboxylative cross-coupling approach allowed for the facile preparation of various C20-arylated derivatives which show promising biological activity in an early bioassay