Infection status and etiological characteristics of diarrheogenic Escherichia coli among diarrhea patients in sentinel hospitals of Fujian Province in 2019

Objective To investigate the infectious status, virulence gene, molecular typing and antibiotic resistance of diarrheogenic Escherichia coli (DEC) in sentinel hospitals of Fujian Province in 2019. Methods Fluorescent polymerase chain reaction (PCR) was used to identify 210 fecal samples of diarrhea patients after isolation of E. coli according to GB 4789. 6-2016, and then pulsed field gel electrophoresis (PFGE) molecular traceability and antibiotic resistance test were conducted on the isolated DEC. Results Thirty two strains of bacteria were detected, with a detection rate of 15.2% (32/210). Among them, enteropathogenic E. coli (EPEC) accounted for 37.5% (12/32), enteroaggregative E. coli (EAEC) for 37.5% (12/32), and enterotoxigenic E. coli (ETEC) for 25.0% (8/32). The results of antibiotic resistance test showed that these 32 strains of bacteria were most resistant to ampicillin, with a resistance rate of 78.1% (25/32), followed by tetracycline and trimethoprim/sulfamethoxazole, with resistance rates of 62.5% (20/32) and 59.4% (19/32), respectively. The multiple antibiotic resistance rate was 50.0% (16/32). The results of PFGE showed that 32 strains of bacteria causing diarrhea were divided into 28 PFGE banding patterns. Among them, 12 strains of EPEC and 12 strains of EAEC were divided into 10 PFGE banding patterns, respectively, and 8 strains of ETEC were divided into 8 PFGE banding patterns. The results of cluster analysis showed that two groups of EPEC strains had 100.0% similar banding patterns, one group of EAEC strains had 100.0% similar banding patterns, and ETEC strains did not have completely consistent banding pattern. Conclusion EPEC and EAEC were the main pathogens of diarrhea in surveillance points of Fujian Province in 2019. The genetic diversity of the strains showed that the genetic relationship between them was relatively distant. The antibiotic resistance of DEC was severe, and the rate of multiple antibiotic resistance was high

Bronchoscopic ethanol injection combined with cryotherapy is an effective treatment for benign airway stenosis caused by endotracheal intubation or tracheotomyc

The benign tracheal stenosis is a challenge in interventional pulmonary disease. Bronchoscopic ethanol injection (BEI) is always used in airway stenosis caused by malignant tracheal tumor. The efficacy and safety of BEI in benign airway stenosis has not been studied before. To compare the safety and efficacy between bronchoscopic icryotherapy and BEI combined with bronchoscopic cryotherapy in the treatment of benign tracheal stenosis. A retrospective study included 61 patients with tracheal stenosis caused by endotracheal intubation and tracheotomy from July 2010 to June 2015 was made. 33 patients received repeated bronchoscopic cryotherapy alone were in Group A, 29 patients underwent repeated cryotherapy combined with BEI were in Group B. Dyspnea index, tracheal diameter were collected before and after treatment. Efficacy and complications were compared in two groups. The changes of tracheal diameter, dyspnea index were significant before and after treatment in both groups (P < 0.05). The long-term cure rate was higher in group B than that in group A (100% vs 84.8%). The average duration for dilated airway stable was much shorter in group B than group A (166±28 days vs 278±32 days, P < 0.05). The average cryotherapy session performed in group B was significantly less than that in group A (22.1±4.7 vs 34.9±6.5, P < 0.05). Meanwhile the complications in group A were seldom, the incidence of complications related to BEI were low in group B (mild chest pain 7.1%, bleeding 3.6% and cough 10.7%). BEI combined with bronchoscopic cryotherapy is an effective minimally invasive choice for releasing the airway obstructive symptoms

A multi‐ethnic analysis of immune‐related gene expression signatures in patients with ovarian clear cell carcinoma

10.1002/path.5769The Journal of Patholog

Bronchoscopic ethanol injection combined with cryotherapy is an effective treatment for benign airway stenosis caused by endotracheal intubation or tracheotomyc: DOI: 10.14800/ics.1171

The benign tracheal stenosis is a challenge in interventional pulmonary disease. Bronchoscopic ethanol injection (BEI) is always used in airway stenosis caused by malignant tracheal tumor. The efficacy and safety of BEI in benign airway stenosis has not been studied before. To compare the safety and efficacy between bronchoscopic icryotherapy and BEI combined with bronchoscopic cryotherapy in the treatment of benign tracheal stenosis. A retrospective study included 61 patients with tracheal stenosis caused by endotracheal intubation and tracheotomy from July 2010 to June 2015 was made. 33 patients received repeated bronchoscopic cryotherapy alone were in Group A, 29 patients underwent repeated cryotherapy combined with BEI were in Group B. Dyspnea index, tracheal diameter were collected before and after treatment. Efficacy and complications were compared in two groups. The changes of tracheal diameter, dyspnea index were significant before and after treatment in both groups (P < 0.05). The long-term cure rate was higher in group B than that in group A (100% vs 84.8%). The average duration for dilated airway stable was much shorter in group B than group A (166±28 days vs 278±32 days, P < 0.05). The average cryotherapy session performed in group B was significantly less than that in group A (22.1±4.7 vs 34.9±6.5, P < 0.05). Meanwhile the complications in group A were seldom, the incidence of complications related to BEI were low in group B (mild chest pain 7.1%, bleeding 3.6% and cough 10.7%). BEI combined with bronchoscopic cryotherapy is an effective minimally invasive choice for releasing the airway obstructive symptoms

A Genetic Linkage Map of BC2 Population Reveals QTL Associated with Plant Architecture Traits in Lagerstroemia

Plant architecture improvement is of great significance in influencing crop yield, harvesting efficiency and ornamental value, by changing the spatial structure of the canopy. However, the mechanism on plant architecture in woody plants is still unclear. In order to study the genetic control of plant architecture traits and promote marker-assisted selection (MAS), a genetic linkage map was constructed, and QTL mapping was performed. In this study, using 188 BC2 progenies as materials, a genetic map of Lagerstroemia was constructed using amplification fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) markers, and the QTLs of four key plant architecture traits (plant height, crown width, primary lateral branch height and internode length) were analyzed. The genetic map contains 22 linkage groups, including 198 AFLP markers and 36 SSR markers. The total length of the genome covered by the map is 1272 cM, and the average distance between markers is 6.8 cM. Three QTLs related to plant height were located in LG1, LG4 and LG17 linkage groups, and the phenotypic variation rates were 32.36, 16.18 and 12.73%, respectively. A QTL related to crown width was located in LG1 linkage group, and the phenotypic variation rate was 18.07%. Two QTLs related to primary lateral branch height were located in the LG1 and LG7 linkage groups, and the phenotypic variation rates were 20.59 and 15.34%, respectively. Two QTLs related to internode length were located in the LG1 and LG20 linkage groups, and the phenotypic variation rates were 14.86 and 9.87%. The results provide a scientific basis for finely mapping genes of plant architecture traits and marker-assisted breeding in Lagerstroemia

Analysis of gene expression signatures identifies prognostic and functionally distinct ovarian clear cell carcinoma subtypes

10.1016/j.ebiom.2019.11.017EBIOMEDICINE50203-21