Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Lipid profiles in the cerebrospinal fluid of rats with 6-hydroxydopamine-induced lesions as a model of Parkinson’s disease

BackgroundParkinson’s disease (PD) is a progressive neurodegenerative disease with characteristic pathological abnormalities, including the loss of dopaminergic (DA) neurons, a dopamine-depleted striatum, and microglial activation. Lipid accumulation exhibits a close relationship with these pathologies in PD.MethodsHere, 6-hydroxydopamine (6-OHDA) was used to construct a rat model of PD, and the lipid profile in cerebrospinal fluid (CSF) obtained from model rats was analyzed using lipidomic approaches.ResultsEstablishment of this PD model was confirmed by apomorphine-induced rotation behaviors, loss of DA neurons, depletion of dopamine in the striatum, and microglial activation after 6-OHDA-induced lesion generation. Unsupervised and supervised methods were employed for lipid analysis. A total of 172 lipid species were identified in CSF and subsequently classified into 18 lipid families. Lipid families, including eicosanoids, triglyceride (TG), cholesterol ester (CE), and free fatty acid (FFA), and 11 lipid species exhibited significantly altered profiles 2 weeks after 6-OHDA administration, and significant changes in eicosanoids, TG, CE, CAR, and three lipid species were noted 5 weeks after 6-OHDA administration. During the period of 6-OHDA-induced lesion formation, the lipid families and species showed concentration fluctuations related to the recovery of behavior and nigrostriatal abnormalities. Correlation analysis showed that the levels of eicosanoids, CE, TG families, and TG (16:0_20:0_18:1) exhibited positive relationships with apomorphine-induced rotation behaviors and negative relationships with tyrosine hydroxylase (TH) expression in the midbrain.ConclusionThese results revealed that non-progressive nigrostriatal degeneration induced by 6-OHDA promotes the expression of an impairment-related lipidomic signature in CSF, and the level of eicosanoids, CE, TG families, and TG (16:0_20:0_18:1) in CSF may reveal pathological changes in the midbrain after 6-OHDA insult

A Temperature Drift Compensation Method for Pulsed Eddy Current Technology

Pulsed eddy current (PEC) technology is another important non-contact nondestructive testing technology for defect detection. However, the temperature drift of the exciting coil has a considerable influence on the precision of PEC testing. The objective of this study is to investigate the temperature drift effect and reduce its impact. The temperature drift effect is analyzed theoretically and experimentally. The temperature drift effect on the peak-to-peak values of the output signal is investigated, and a temperature compensation method is proposed to reduce the effect of temperature variation. The results show that temperature drift has a negative impact on PEC testing and the temperature compensation method can effectively reduce the effect of temperature drift

A Representative User-centric Dataset of 10 Million GitHub Developers

Using GitHub APIs, we construct an unbiased dataset of over 10 million GitHub users. The data was collected between Jul. 20 and Aug. 27, 2018, covering 10,649,574 users, 118,602,740 commits, and 20,999,258 repositories. Each data entry is stored in JSON format, representing one GitHub user, and containing the descriptive information in the user’s profile page, the information of her commit activities and created/forked public repositories

Mechanism of SQQX Decoction’s Protective Effect on SHR: A Serum Metabolomics-Based Analysis

SangQiQingXuan (SQQX) decoction is a pharmaceutical preparation exerting good therapeutic efficacy on high blood pressure (BP) and has widely been accepted in primarily hypertensive patients as a herbal formula prescribed by Professor Li Huang from China-Japan Friendship Hospital according to her 30-year clinical experience. A previous study showed that SQQX could reduce BP by decreasing levels of many inflammatory factors such as transforming growth factor beta (TGFβ) and elevating peroxisome proliferator activated receptor (PPAR) expression. However, a research focusing on SQQX’s protection against HTN from a metabolomic perspective has never been done before. This study aimed to figure out the metabolic profiling variations due to oral administration of SQQX in spontaneous hypertensive rat (SHR) models and to find out the optimal dosage of SQQX. SHR in the intervention group orally received SQQX extract of three doses, namely, the low- (5.25 g/kg/d), middle- (10.5 g/kg/d), and high-dosage groups (21 g/kg/d) for 90 days. Rats were sacrificed at the end of the experiment, and their serum was collected for further examination. Serum metabolic profiling variations were analyzed using ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC/MS). Results showed that dealing with SQQX remarkably decreased systolic blood pressure (SBP) of SHRs and the high-dosage group was with the best therapeutic effect where a total of 11 metabolites were markedly changed in contrast to the model group. Orthogonal partial least square discriminant analysis (OPLS-DA) score plot showed that the 5 groups of serum samples were divided into 5 categories, and the metabolic trajectory of the high-dosage SQQX group was inclined to move to the control group. Glycochenodeoxycholic acid, nicotinamide-N-oxide, and tryptophan betaine might be biomarkers that specifically marked the protective effects of SQQX against high BP mainly involving in cholesterol metabolism, primary bile acid biosynthesis, bile secretion, and nicotinate and nicotinamide metabolism. To conclude, SQQX has a protective effect on SHR, which may be partially correlated to restoration of perturbed metabolism in serum

Evolution of the Chenglingji–Datong Channel in the Middle and Lower Reaches of the Yangtze River and Its Drivers

In recent years, the water–sand composition of the Yangtze River channel has changed due to the influence of human factors, especially the construction of water reservoirs such as the Three Gorges Project. Changing water–sand conditions have a long-term impact on the shaping of the river channel morphology in the middle and lower reaches of the Yangtze River, and the erosion retreat of local river sections has caused great harm to embankment projects. This paper focuses on the river evolution mechanism of the river channel from Chenglingji to Datong in the middle and lower reaches of the Yangtze River over the past 31 years. Landsat remote sensing images from 1989–2019 were used to extract and interpret water bodies, river shorelines, and central bars in the study area using the Modified Normalized Difference Water Index (MNDWI) combined with visual interpretation. We used near analysis to study the morphological evolution characteristics of the river, the channel, and selected typical river reaches for comparative analysis. We found out that the overall change in river morphology between 1989 and 2019 was small in the horizontal direction, but the local area changed significantly. Considerable scouring occurred in the vertical direction. Combining hydrological and meteorological data, we investigated the effects of the Three Gorges Dam, instream sand mining, boundary conditions, vegetation cover on both sides of the riverbanks, and aspects of storm flooding in the watershed on the evolution of the river. The study indicated that the geological conditions on both sides of the river, the implementation of the bank protection project, and the improvement of vegetation cover on both sides of the river have made the riverbanks more resistant to scouring. However, heavy rainfall floods, the operation of the Three Gorges Reservoir, and sand mining activities in the river channel make the river channel more susceptible to scouring. Based on the calculation of the slope change rate of the accumulated volume, it was found that the runoff is mainly influenced by precipitations, while the sand transport is mainly affected by human activities. This study shows that natural and anthropogenic activities affect the equilibrium state of the river’s water and sediment to varying degree

Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization

10.1021/acsami.5b03766ACS APPLIED MATERIALS & INTERFACES72714965-1497

CXCL10 plays a key role as an inflammatory mediator and a non-invasive biomarker of non-alcoholic steatohepatitis

Background & Aims Perpetuate liver inflammation is crucial in the pathogenesis of non-alcoholic steatohepatitis (NASH). Expression of CXCL10, a pro-inflammatory cytokine, correlates positively with obesity and type 2 diabetes. Whether CXCL10 plays a

Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization

Fluorescent organic dots are emerging as promising bioimaging reagents because of their high brightness, good photostability, excellent biocompatibility, and facile surface functionalization. Organic dots with large two-photon absorption (TPA) cross sections are highly desired for two-photon fluorescence microscopy. In this work, we report two biocompatible and photostable organic dots fabricated by encapsulating tetraphenylethene derivatives within DSPE-PEG matrix. The two organic dots show absorption maxima at 425 and 483 nm and emit green and red fluorescence at 560 and 645 nm, with high fluorescence quantum yields of 64% and 22%, respectively. Both organic dots exhibit excellent TPA property in the range of 800–960 nm, affording upon excitation at 820 nm remarkably large TPA cross sections of 1.2 × 10⁶ and 2.5 × 10⁶ GM on the basis of dot concentration. The bare fluorophores and their organic dots are biocompatible and have been used to stain living cells for one- and two-photon fluorescence bioimagings. The cRGD-modified organic dots can selectively target integrin α_vβ₃ overexpressing breast cancer cells for targeted imaging. The organic dots are also applied for real-time two-photon fluorescence in vivo visualization of the blood vasculature of mouse ear, providing the spatiotemporal information about the whole blood vascular network. These results demonstrate that the present fluorescent organic dots are promising candidates for living cell and tissue imaging