Vestibular Function in Children and Adults Before and After Unilateral or Sequential Bilateral Cochlear Implantation

Background: Cochlear implantation (CI) helps patients with severe or profound sensorineural hearing loss (SNHL) restore hearing and speech abilities. However, some patients exhibit abnormal vestibular functions with symptoms such as dizziness or balance disorders, after CI. Whether age at CI and CI approach (unilateral or sequential bilateral) affect vestibular functions in users with cochlear implants remains unclear.Objectives: To investigate the vestibular functions in children and adults before and after unilateral or sequential bilateral CI.Materials and Methods: Thirty-seven patients with severe or profound SNHL who were candidates for a first- or second-side CI were divided into three groups: first-side CI-implanted adults (≥18 years), first-side CI-implanted children (6–17 years), and second-side CI-implanted children (6–17 years). All cases were implanted with the round window approach to minimize damage to the intra-cochlear structures. The caloric test, vestibular evoked myogenic potential (VEMP) test, video head impulse test (vHIT), Dizziness Handicap Inventory (DHI), Pediatric Vestibular Symptom Questionnaire (PVSQ), and audiometric tests were performed before and 1 month after CI.Results: The abnormal rates of caloric test and VEMP test after CI in the first-side CI-implanted adults and children significantly increased compared with those before CI. The pre-implantation VEMP test showed significantly higher abnormal rates between first- and second-side CI-implanted children. No other significant differences of abnormal rates between first- and second-side CI-implanted children or between first-side CI-implanted adults and children were found. In second-side CI-implanted children, PVSQ scores significantly increased at day 3 post-implantation but decreased at day 30.Conclusion: CI has a negative effect on the results of caloric and VEMP tests, but not on vHIT, indicating that the otolith and low-frequency semicircular canal (SCC) are more vulnerable to damage from CI. The alterations of vestibular functions resulting from CI surgery may be independent of age at CI and CI approach (unilateral or sequential bilateral). Long-term impacts on the vestibular function from CI surgery, as well as the chronic electrical stimulation to the cochlea, are still to be investigated

Recombinant Cyclodextrinase from Thermococcus kodakarensis

A gene encoding a cyclodextrinase from Thermococcus kodakarensis KOD1 (CDase-Tk) was identified and characterized. The gene encodes a protein of 656 amino acid residues with a molecular mass of 76.4 kDa harboring four conserved regions found in all members of the α-amylase family. A recombinant form of the enzyme was purified by ion-exchange chromatography, and its catalytic properties were examined. The enzyme was active in a broad range of pH conditions (pHs 4.0–10.0), with an optimal pH of 7.5 and a temperature optimum of 65°C. The purified enzyme preferred to hydrolyze β-cyclodextrin (CD) but not α- or γ-CD, soluble starch, or pullulan. The final product from β-CD was glucose. The Vmax and Km values were 3.13 ± 0.47 U mg−1 and 2.94 ± 0.16 mg mL−1 for β-CD. The unique characteristics of CDase-Tk with a low catalytic temperature and substrate specificity are discussed, and the starch utilization pathway in a broad range of temperatures is also proposed

Prevalence of preoperative cognitive impairment among elderly thoracic surgery patients and association with postoperative delirium: a prospective observational study

BackgroundPreoperative cognitive impairment (PCI) may increase the incidence of postoperative delirium (POD), yet screening for cognitive impairment is rarely performed. This study hypothesized that Mini-Cog for preoperative cognitive impairment screening predicts postoperative delirium.MethodsThe prospective observational study recruited 153 elderly patients presenting for elective thoracic surgery. Cognitive function of these patients was screened using Mini-Cog preoperatively. We considered that patients with Mini-Cog scores ≤ 3 had cognitive impairment. Delirium was assessed using the Short CAM scale on postoperative days 1–5.ResultsOf the 153 participants, 54 (35.3%) were assigned to the PCI group, and 99 (64.7%) were assigned to the Normal group. Place of residence, education level, and history of hypertension were significantly different between the two groups (P < 0.05). 51 (33.3%) patients developed POD. Multifactorial analysis revealed that PCI (OR = 2.37, P = 0.028), older age (OR = 1.13, P = 0.009), ASA grade III (OR = 2.75, P = 0.012), and longer duration of anesthesia (OR = 1.01, P = 0.007) were associated with POD.ConclusionPreoperative cognitive impairment is strongly associated with POD. Mini-Cog could be recommended for screening PCI.Clinical trial registrationClinicalTrials.gov, identifier NCT05798767

Effect of the shelterbelt along the Tarim Desert Highway on air temperature and humidity

The temperature and humidity of the shelterbelt micro-climate on both horizontal and vertical scales in the extremely drought area were measured with multiple HOBO temperature and humidity automatic observation equipments in the hinterland of the Taklimakan Desert. The results show that the shelterbelt ecosystem of the desert highway plays typical micro-climate adjustment rolesin stabilizing surface air temperature and increasing air humidity, and so on. Solar radiation significantly affects both temperature and humidity of surface layers, and it has a positive correlation with the temperature but a negative correlation with the air humidity. When it is cloudy, the weather has a great impact on keeping temperature and humidity in the shelterbelt. The shelterbelt also significantly influences the environment, and the micro-climate in the belt has an obvious characteristic of cooling and humidification: compared with the original sand area, the temperature in the shelterbelt is always lower and the humidity is always higher. Moreover, the temperature range at the shelterbelt edge is greater than that in the sand area, but the humidity is always higher. Our conclusion is that the vertical-effect range of temperature of the shelterbelts is 4-10 m, and the humidity range is 6 to 8 m; the horizontal-effect range of temperature is 16 m and the humidity range is about 24 m

The salt accumulation at the shifting aeolian sandy soil surface with high salinity groundwater drip irrigation in the hinterland of the Taklimakan Desert

The EC analysis and water serial sampling was performed in the Tarim Desert Highway shelterbelt to explore the water and salt dynamics of the shallow aeolian sandy soil (0-30cm) under high salinity groundwater drip irrigation. It was found that in one irrigation cycle, the EC of the shallow shifting aeolian sandy soil (0-30cm) increased while the water content decreased. The EC of the surface aeolian sandy soil at the wetting front was far greater than that of the wetting area or the outside of the wetting area. During the irrigation cycle, the EC of the wetting front and the wetting area changed at a significant magnitude, whereas the EC of the outside of the wetting area remained largely steady. The horizontal influence distance of drip irrigation on the salt accumulation at the soil surface was about 100 cm, and the vertical influence depth was 5 cm. The three most abundant ions in the accumulated salt at the aeolian sandy soil surface were Na+, Cl- and SO42-. The salt accumulation at the soil surface was influenced by air temperature, wind speed, mineralization of irrigation water, sand burial thickness, soil texture, and litter content

Effects of natural covers on soil evaporation of the shelterbelt along the Tarim Desert Highway

The control of soil evaporation is one of important approaches to save water. The artificially simulated evaporation experiments have been conducted in the hinterland of the Taklimakan Desert to reveal the effects of the natural covers on the soil evaporation of the Tarim Desert Highway shelterbelt as well as provide some insights in the efficient utilization of water resources and optimization of irrigation systems. The results showed that (1) All the covers, including the sand deposit, the salt crust, the litter, the sand-litter mixed layer and so on, can significantly inhibit the soil water evaporation. Specifically, the daily evaporation, the total evaporation, and the evaporation rate in covered sands were much smaller than that of sands without cover. The cover inhibition effects increased with the cover thickness. Particularly, the soil evaporation of the covered sands was less affected by external and internal factors than that of the bare sands. Moreover, the variation of daily evaporation of covered sands was smaller than that of bare sands. The cumulative evaporation varied linearly with time in the covered sands whereas it varied logarithmically in the bare sands. In addition, the soil evaporation in the bare sands showed significantly different characteristics in the early and late stages of the evaporation. (2) All the covers exhibited the significant inhibiting effect on the soil evaporation, and the inhibition efficiency increased logarithmically with the cover thickness. However, as the cover thickness was above a certain value, the increase in the inhibition efficiency was slow. Particularly, at a cover thickness of 2 cm, there was no obvious difference in the inhibition efficiency among all kinds of covers. The maximum inhibition efficiency as calculated from the daily evaporation on the first day of irrigation was: sand-litter mixed layer (79.92%) > litter layer (78.96%) > salt crust (75.58%) > sand bed (74.11%), whereas the average inhibiting efficiency as calculated from the cumulative soil evaporation at the end of an irrigation cycle (the fourth day) was: salt crust (67.78%) > sand-litter mixed layer (66.72%) > sand deposit (63.28%) > litter layer (61.74%)

Flame-Spray-Made Undoped Zinc Oxide Films for Gas Sensing Applications

Using zinc naphthenate dissolved in xylene as a precursor undoped ZnO nanopowders were synthesized by the flame spray pyrolysis technique. The average diameter and length of ZnO spherical and hexagonal particles were in the range of 5 to 20 nm, while ZnO nanorods were found to be 5–20 nm wide and 20–40 nm long, under 5/5 (precursor/oxygen) flame conditions. The gas sensitivity of the undoped ZnO nanopowders towards 50 ppm of NO2, C2H5OH and SO2 were found to be 33, 7 and 3, respectively. The sensors showed a great selectivity towards NO2 at high working temperature (at 300 °C), while small resistance variations were observed for C2H5OH and SO2, respectively

PIF4–Mediated Activation of YUCCA8 Expression Integrates Temperature into the Auxin Pathway in Regulating Arabidopsis Hypocotyl Growth

Higher plants adapt their growth to high temperature by a dramatic change in plant architecture. It has been shown that the transcriptional regulator phytochrome-interacting factor 4 (PIF4) and the phytohormone auxin are involved in the regulation of high temperature–induced hypocotyl elongation in Arabidopsis. Here we report that PIF4 regulates high temperature–induced hypocotyl elongation through direct activation of the auxin biosynthetic gene YUCCA8 (YUC8). We show that high temperature co-upregulates the transcript abundance of PIF4 and YUC8. PIF4–dependency of high temperature–mediated induction of YUC8 expression as well as auxin biosynthesis, together with the finding that overexpression of PIF4 leads to increased expression of YUC8 and elevated free IAA levels in planta, suggests a possibility that PIF4 directly activates YUC8 expression. Indeed, gel shift and chromatin immunoprecipitation experiments demonstrate that PIF4 associates with the G-box–containing promoter region of YUC8. Transient expression assay in Nicotiana benthamiana leaves support that PIF4 directly activates YUC8 expression in vivo. Significantly, we show that the yuc8 mutation can largely suppress the long-hypocotyl phenotype of PIF4–overexpression plants and also can reduce high temperature–induced hypocotyl elongation. Genetic analyses reveal that the shy2-2 mutation, which harbors a stabilized mutant form of the IAA3 protein and therefore is defective in high temperature–induced hypocotyl elongation, largely suppresses the long-hypocotyl phenotype of PIF4–overexpression plants. Taken together, our results illuminate a molecular framework by which the PIF4 transcriptional regulator integrates its action into the auxin pathway through activating the expression of specific auxin biosynthetic gene. These studies advance our understanding on the molecular mechanism underlying high temperature–induced adaptation in plant architecture