Isotopic Application in High Saline Conditions

Evaporite minerals record the hydrogeochemical conditions in which they precipitated. And therefore they can be used to reconstruct the paleoclimate and paleoenvironments. Evaporite minerals are also major sources of industrial minerals including gypsum, halite, borates, lithium concentrates, and others. Because of their scientific significance and economic importance, evaporite minerals and their isotopic hydrochemical processes linked to their formation have been the focus of many geologists and paleoclimatologists. This chapter will discuss the application of isotopes of hydrogen, oxygen, sulfur, strontium, and boron in saline conditions. This will include the following: the δ18O and δD of hydrated water of gypsum and their paleoclimate since 2.2 Ma in the Qaidam Basin, NE Tibetan Plateau; the δ18O and δD of the interlayer water of clay minerals in salar lacustrine sediments; and the 87Sr/86Sr, δ34S, and δ11B of halite from evaporite deposits in Khorat Plateau, Laos, and Yunnan and their application in the origins of brine

Measurement of the Near Field Distribution of a Microwave Horn Using a Resonant Atomic Probe

We measure the near field distribution of a microwave horn with a resonant atomic probe. The microwave field emitted by a standard microwave horn is investigated utilizing Rydberg electromagnetically inducted transparency (EIT), an all-optical Rydberg detection, in a room temperature caesium vapor cell. The ground 6S1/2 , excited 6P3/2 , and Rydberg 56D5/2 states constitute a three-level system, used as an atomic probe to detect microwave electric fields by analyzing microwave dressed Autler–Townes (AT) splitting. We present a measurement of the electric field distribution of the microwave horn operating at 3.99 GHz in the near field, coupling the transition 56D5/2→57P3/2 . The microwave dressed AT spectrum reveals information on both the strength and polarization of the field emitted from the microwave horn simultaneously. The measurements are compared with field measurements obtained using a dipole metal probe, and with simulations of the electromagnetic simulated software (EMSS). The atomic probe measurement is in better agreement with the simulations than the metal probe. The deviation from the simulation of measurements taken with the atomic probe is smaller than the metal probe, improving by 1.6 dB. The symmetry of the amplitude distribution of the measured field is studied by comparing the measurements taken on either side of the field maxima

Dephasing of ultracold cesium 80D5/280D_{5/2}-Rydberg Electromagnetically Induced Transparency

We study Rydberg electromagnetically induced transparency (EIT) of a cascade three-level atom involving 80$D_{5/2}$ state in a strong interaction regime employing a cesium ultracold cloud. In our experiment, a strong coupling laser couples 6$P_{3/2}$ to 80$D_{5/2}$ transition, while a weak probe, driving 6$S_{1/2}$ to 6$P_{3/2}$ transition, probes the coupling induced EIT signal. At the two-photon resonance, we observe that the EIT transmission decreases slowly with time, which is a signature of interaction induced metastability. The dephasing rate $\gamma_{\rm OD}$ is extracted with optical depth OD = $\gamma_{\rm OD}t$. We find that the optical depth linearly increases with time at onset for a fixed probe incident photon number $R_{\rm in}$ before saturation. The dephasing rate shows a nonlinear dependence on $R_{\rm in}$. The dephasing mechanism is mainly attributed to the strong dipole-dipole interactions, which leads to state transfer from $nD_{5/2}$ to other Rydberg states. We demonstrate that the typical transfer time $\tau_{0(80D)}$ obtained by the state selective field ionization technique is comparable with the decay time of EIT transmission $\tau_{0({\rm EIT})}$. The presented experiment provides a useful tool for investigating the strong nonlinear optical effects and metastable state in Rydberg many-body systems.Comment: 7 pages, 5 figure

The role of EGFR double minutes in modulating the response of malignant gliomas to radiotherapy.

EGFR amplification in cells having double minute chromosomes (DM) is commonly found in glioblastoma multiforme (GBM); however, how much it contributes to the current failure to treat GBM successfully is unknown. We studied two syngeneic primary cultures derived from a GBM with and without cells carrying DM, for their differential molecular and metabolic profiles, in vivo growth patterns, and responses to irradiation (IR). Each cell line has a distinct molecular profile consistent with an invasive "go" (with DM) or angiogenic "grow" phenotype (without DM) demonstrated in vitro and in intracranial xenograft models. Cells with DM were relatively radio-resistant and used higher glycolytic respiration and lower oxidative phosphorylation in comparison to cells without them. The DM-containing cell was able to restore tumor heterogeneity by mis-segregation of the DM-chromosomes, giving rise to cell subpopulations without them. As a response to IR, DM-containing cells switched their respiration from glycolic metabolism to oxidative phosphorylation and shifted molecular profiles towards that of cells without DM. Irradiated cells with DM showed the capacity to alter their extracellular microenvironment to not only promote invasiveness of the surrounding cells, regardless of DM status, but also to create a pro-angiogenic tumor microenvironment. IR of cells without DM was found primarily to increase extracellular MMP2 activity. Overall, our data suggest that the DM-containing cells of GBM are responsible for tumor recurrence due to their high invasiveness and radio-resistance and the mis-segregation of their DM chromosomes, to give rise to fast-growing cells lacking DM chromosomes

Nanotechnology-based non-viral vectors for gene delivery in cardiovascular diseases

Gene therapy is a technique that rectifies defective or abnormal genes by introducing exogenous genes into target cells to cure the disease. Although gene therapy has gained some accomplishment for the diagnosis and therapy of inherited or acquired cardiovascular diseases, how to efficiently and specifically deliver targeted genes to the lesion sites without being cleared by the blood system remains challenging. Based on nanotechnology development, the non-viral vectors provide a promising strategy for overcoming the difficulties in gene therapy. At present, according to the physicochemical properties, nanotechnology-based non-viral vectors include polymers, liposomes, lipid nanoparticles, and inorganic nanoparticles. Non-viral vectors have an advantage in safety, efficiency, and easy production, possessing potential clinical application value when compared with viral vectors. Therefore, we summarized recent research progress of gene therapy for cardiovascular diseases based on commonly used non-viral vectors, hopefully providing guidance and orientation for future relevant research

Dephasing of ultracold cesium 80í µí°· 5/2 -Rydberg Electromagnetically Induced Transparency

We study Rydberg electromagnetically induced transparency (EIT) of a cascade three-level atom involving 80í µí°· 5/2 state in a strong interaction regime employing a cesium ultracold cloud. In our experiment, a strong coupling laser couples 6í µí± 3/2 to 80í µí°· 5/2 transition, while a weak probe, driving 6í µí± 1/2 to 6í µí± 3/2 transition, probes the coupling induced EIT signal. At the two-photon resonance, we observe that the EIT transmission decreases slowly with time, which is a signature of interaction induced metastability. The dephasing rate í µí»¾ OD is extracted with optical depth OD = í µí»¾ OD í µí±¡. We find that the optical depth linearly increases with time at onset for a fixed probe incident photon number í µí± in before saturation. The dephasing rate shows a nonlinear dependence on í µí± in. The dephasing mechanism is mainly attributed to the strong dipole-dipole interactions, which leads to state transfer from í µí±í µí°· 5/2 to other Rydberg states. We demonstrate that the typical transfer time í µí¼ 0(80í µí°·) obtained by the state selective field ionization technique is comparable with the decay time of EIT transmission í µí¼ 0(EIT). The presented experiment provides a useful tool for investigating the strong nonlinear optical effects and metastable state in Rydberg many-body systems