Will EGFRvIII and neuronal-derived EGFR be targets for imipramine?

Tricyclic antidepressant is an old and well-established therapeutic agent with a good safety profile, making them an excellent candidate for repurposing. In light of the growing understanding of the importance of nerves in the development and progression of cancer, attention is now being turned to using nerve-targeting drugs for the treatment of cancer, particularly TCAs. However, the specific mechanism by which antidepressants affect the tumor microenvironment of glioblastoma (GBM) is still unclear. Here, we combined bulk RNA sequencing, network pharmacology, single-cell sequencing, molecular docking and molecular dynamics simulation to explore the potential molecular mechanism of imipramine in the treatment of GBM. We first revealed that the imipramine treatment is presumed to target EGFRvIII and neuronal-derived EGFR, which may play a pivotal role in treating GBM by reducing the GABAergic synapse and vesicle-mediated release and other processes thereby modulating immune function. The novel pharmacological mechanisms might provide further research directions

Real-Time Global Flood Estimation Using Satellite-Based Precipitation and a Coupled Land Surface and Routing Model

A widely used land surface model, the Variable Infiltration Capacity (VIC) model, is coupled with a newly developed hierarchical dominant river tracing-based runoff-routing model to form the Dominant river tracing-Routing Integrated with VIC Environment (DRIVE) model, which serves as the new core of the real-time Global Flood Monitoring System (GFMS). The GFMS uses real-time satellite-based precipitation to derive flood monitoring parameters for the latitude band 50 deg. N - 50 deg. S at relatively high spatial (approximately 12 km) and temporal (3 hourly) resolution. Examples of model results for recent flood events are computed using the real-time GFMS (http://flood.umd.edu). To evaluate the accuracy of the new GFMS, the DRIVE model is run retrospectively for 15 years using both research-quality and real-time satellite precipitation products. Evaluation results are slightly better for the research-quality input and significantly better for longer duration events (3 day events versus 1 day events). Basins with fewer dams tend to provide lower false alarm ratios. For events longer than three days in areas with few dams, the probability of detection is approximately 0.9 and the false alarm ratio is approximately 0.6. In general, these statistical results are better than those of the previous system. Streamflow was evaluated at 1121 river gauges across the quasi-global domain. Validation using real-time precipitation across the tropics (30 deg. S - 30 deg. N) gives positive daily Nash-Sutcliffe Coefficients for 107 out of 375 (28%) stations with a mean of 0.19 and 51% of the same gauges at monthly scale with a mean of 0.33. There were poorer results in higher latitudes, probably due to larger errors in the satellite precipitation input

Influence of climatic variables on maize grain yield and its components by adjusting the sowing date

Yield and its components are greatly affected by climate change. Adjusting the sowing date is an effective way to alleviate adverse effects and adapt to climate change. Aiming to determine the optimal sowing date of summer maize and clarify the contribution of climatic variables to grain yield and its components, a consecutive 4-year field experiment was conducted from 2016 to 2019 with four sowing dates at 10-day intervals from 5 June to 5 July. Analysis of historical meteorological data showed that more solar radiation (SR) was distributed from early June to mid-August, and the maximum temperature (Tmax) > 32°C appeared from early July to late August, which advanced and lasted longer in 1991–2020 relative to 1981–1990. Additionally, the precipitation was mainly distributed from early June to late July. The climate change in the growing season of summer maize resulted in optimal sowing dates ranging from 5 June to 15 June, with higher yields and yield stability, mainly because of the higher kernel number per ear and 1,000-grain weight. The average contribution of kernel number per ear to grain yield was 58.7%, higher than that of 1,000-grain weight (41.3%). Variance partitioning analysis showed that SR in 15 days pre-silking to 15 days post-silking (SS) and silking to harvest (SH) stages significantly contributed to grain yield by 63.1% and 86.4%. The extreme growing degree days (EDD) > 32°C, SR, precipitation, and diurnal temperature range (DTR) contributed 20.6%, 22.9%, 14.5%, and 42.0% to kernel number per ear in the SS stage, respectively. Therefore, we concluded that the early sowing dates could gain high yield and yield stability due to the higher SR in the growing season. Meanwhile, due to the decreasing trend in SR and increasing Tmax trend in this region, in the future, new maize varieties with high-temperature resistance, high light efficiency, shade tolerance, and medium-season traits need to be bred to adapt to climate change and increased grain yield

Risk of COVID-19 Transmission Aboard Aircraft: An Epidemiological Analysis Based on the National Health Information Platform.

OBJECTIVES This study aims to investigate the risk of COVID-19 transmission on aircraft. METHODS We obtained data on all international flights to Lanzhou, China, from June 1 to August 1, 2020, through the Gansu Province National Health Information Platform and the official website of the Gansu Provincial Center for Disease Control and Prevention. Statistical analysis was then performed. RESULTS Three international flights arrived in Lanzhou. The flights had a total of 700 passengers, of whom 405 (57.9%) were male and 80 (11.4%) were children below age fourteen. Twenty-seven (3.9%) passengers were confirmed to have COVID-19. Confirmed patients were primarily male (17, 65.4%) with a median age of 27.0 years. The majority of confirmed cases were seated in the middle rows of the economy class, or near public facility areas such as restrooms and galleys. The prevalence of COVID-19 did not differ between passengers sitting on window, aisle or middle seats. Compared with passengers sitting on the same row up to two rows behind a confirmed case, passengers seated in the two rows ahead a confirmed case were at a slightly higher risk of being infected. CONCLUSIONS COVID-19 may be transmitted during a passenger flight, although there is still no direct evidence

Defining the sizes of airborne particles that mediate influenza transmission in ferrets

Epidemics and pandemics of influenza are characterized by rapid global spread mediated by non-mutually exclusive transmission modes. The relative significance between contact, droplet, and airborne transmission is yet to be defined, a knowledge gap for implementing evidence-based infection control measures. We devised a transmission chamber that separates virus-laden particles by size and determined the particle sizes mediating transmission of influenza among ferrets through the air. Ferret-to-ferret transmission was mediated by airborne particles larger than 1.5 µm, consistent with the quantity and size of virus-laden particles released by the donors. Onward transmission by donors was most efficient before fever onset and may continue for 5 days after inoculation. Multiple virus gene segments enhanced the transmissibility of a swine influenza virus among ferrets by increasing the release of virus-laden particles into the air. We provide direct experimental evidence of influenza transmission via droplets and fine droplet nuclei, albeit at different efficiency

Jute-derived microporous/mesoporous carbon with ultra-high surface area using a chemical activation process

Here, we report the synthesis of nanoporous carbons (NCs) derived from a low-cost and renewable biomass, jute, by a chemical activation process using KOH. Jute is one of the least expensive and most abundant crops, with a staggering 2.8 million metric tons of jute produced each year. In this study, we synthesize NCs from three different parts of jute fibers through a chemical activation technique using KOH. The NCs prepared from the bottom portion of the fiber show a high surface area (2682 m g) with the presence of both micropores and mesopores. The ultra-high surface area of jute makes it an economically viable, environmentally friendly precursor for NCs, with a wide variety of applications from energy storage to environmental and biomedical applications

“Learn to Conserve Your Passion and Care”: Exploring the Emotional Labor of Special-Post Teachers in Rural China

In recent decades, the growing trend of post-structuralist research on teacher emotional labor has offered a discursive lens to elucidate rural teachers’ identities and their teaching practices. To date, however, few studies have explored the emotional labor of special-post teachers in rural China. Through a post-structuralist framework, this study aimed to explore the emotional labor of special-post teachers. Ethnographic qualitative data from a rural primary school in northern China showed that special-post teachers experienced various emotional conflicts embedded in multiple discourses. As teaching experience increases, special-post teachers obtain agentive emotional and practical responses to lighten their negative emotional burden for work. The findings suggested that the role overload and conflicts of special-post teachers were especially prominent in the social context of the urban–rural dichotomy. Emotional reflexivity and vulnerability of special-post teachers in their identity construction as educator, professional-service-provider, and also passer-by were also discussed

Study of the Subsidence Width Influence on the Geotextile Control of a Subgrade Collapse Based on a Half-Symmetric Model Test

The geotextile can be used to treat a subgrade collapse in karst areas. The subsidence width is an important factor affecting the geotextile to treat subgrade collapses. However, the available studies on the influence of the subsidence width on geotextile treatment settlement are limited. To study the effect of the subsidence width on the geotextile control of subgrade collapses, the half-symmetric model test had been established. To make up for the deficiencies of the model test, the optimized subsidence width was probed through a numerical calculation under ten different situations conducted by the finite element analyses. Previous full-section model test results were used to verify the rationality of the half-symmetric model and calibrate the input parameters of the numerical models. The influence of the subsidence width on soil pressure, tensile force and deformation of the geotextile, and soil settlement was analyzed. With the increase of the subsidence width, more loads of the subsidence area were transferred to a stable area via the geotextile, the vertical normal stress at the edge increased rapidly, the tensile force of the geotextile and vertical soil displacement in the subsidence area increased noticeably. When the anchorage ratio of L ≤ 2.0B, the geotextile fracture or soil failure occurred during the model test which indicated the geotextile treatment of the subgrade collapse was not suitable for projects with an anchorage ratio of L ≤ 2.0B. The geotextile might be reaching the limit of its tensile stiffness when the anchorage ratio of L = 2.22B. This is providing an insight into the treatment of a subgrade collapse in karst areas using geotextile

Study of the Subsidence Width Influence on the Geotextile Control of a Subgrade Collapse Based on a Half-Symmetric Model Test

The geotextile can be used to treat a subgrade collapse in karst areas. The subsidence width is an important factor affecting the geotextile to treat subgrade collapses. However, the available studies on the influence of the subsidence width on geotextile treatment settlement are limited. To study the effect of the subsidence width on the geotextile control of subgrade collapses, the half-symmetric model test had been established. To make up for the deficiencies of the model test, the optimized subsidence width was probed through a numerical calculation under ten different situations conducted by the finite element analyses. Previous full-section model test results were used to verify the rationality of the half-symmetric model and calibrate the input parameters of the numerical models. The influence of the subsidence width on soil pressure, tensile force and deformation of the geotextile, and soil settlement was analyzed. With the increase of the subsidence width, more loads of the subsidence area were transferred to a stable area via the geotextile, the vertical normal stress at the edge increased rapidly, the tensile force of the geotextile and vertical soil displacement in the subsidence area increased noticeably. When the anchorage ratio of L ≤ 2.0B, the geotextile fracture or soil failure occurred during the model test which indicated the geotextile treatment of the subgrade collapse was not suitable for projects with an anchorage ratio of L ≤ 2.0B. The geotextile might be reaching the limit of its tensile stiffness when the anchorage ratio of L = 2.22B. This is providing an insight into the treatment of a subgrade collapse in karst areas using geotextile