Polymeric 2,6-bis(benzimidazol-2-yl)pyridine -RuCl3 complex as a catalyst for the aerobic oxidative self-condensation of amines to imines

A polymer ruthenium complex pbbp-RuCl3 has been easily synthesized from the direct coordination of RuCl3 with polymer constituted by tridentate 2,6-bis(benzimidazol-2-yl)pyridine unit (pbbp). As a heterogeneous catalyst, pbbp-RuCl3 showed high efficiency in aerobic oxidative self-condensation of primary amines to imines. This heterogeneous catalyst can be easily recovered and exhibits good reusability in the reaction

CCL5 promotes LFA-1 expression in Th17 cells and induces LCK and ZAP70 activation in a mouse model of Parkinson’s disease

BackgroundParkinson’s disease (PD), which is associated to autoimmune disorders, is characterized by the pathological deposition of alpha-synuclein (α-Syn) and loss of dopaminergic (DA) neurons. Th17 cells are thought to be responsible for the direct loss of DA neurons. C-C chemokine ligand 5 (CCL5) specifically induces Th17 cell infiltration into the SN. However, the specific effect of CCL5 on Th17 cells in PD and the relationship between CCL5 and lymphocyte function-associated antigen-1 (LFA-1) expression in Th17 cells are unknown.MethodsWe evaluated the effects of CCL5 on LFA-1 expression in Th17 cells in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and examined Th17 cell differentiation upon CCL5 stimulation in vitro. Furthermore, we assessed the effects of CCL5 on tyrosine kinase zeta-chain-associated protein kinase 70 (ZAP70) and lymphocyte-specific protein tyrosine kinase (LCK) activity in CCL5-stimulated Th17 cells in vivo and in vitro.ResultsCCL5 increased the proportion of peripheral Th17 cells in MPTP-treated mice, LFA-1 expression on Th17 cells, and Th17 cell levels in the SN of MPTP-treated mice. CCL5 promoted Th17 cell differentiation and LFA-1 expression in naive T cells in vitro. Moreover, CCL5 increased Th17 cell differentiation and LFA-1 expression by stimulating LCK and ZAP70 activation in naive CD4+ T cells. Inhibiting LCK and ZAP70 activation reduced the proportion of peripheral Th17 cells and LFA-1 surface expression in MPTP-treated mice, and Th17 cell levels in the SN also significantly decreased.ConclusionCCL5, which increased Th17 cell differentiation and LFA-1 protein expression by activating LCK and ZAP70, could increase the Th17 cell number in the SN, induce DA neuron death and aggravate PD

Polymeric 2,6-bis(benzimidazol-2-yl)pyridine-RuCl₃ complex as a catalyst for aerobic oxidative self-condensation of amines to imines

1242-1248A polymer ruthenium complex pbbp-RuCl3 has been easily synthesized from the direct coordination of RuCl3 with polymer constituted by tridentate 2,6-bis(benzimidazol-2-yl)pyridine unit (pbbp). As a heterogeneous catalyst, pbbp-RuCl3 showed high efficiency in aerobic oxidative self-condensation of primary amines to imines. This heterogeneous catalyst can be easily recovered and exhibits good reusability in the reaction

An analysis method for residential electricity consumption behavior based on UMAP-CRITIC feature optimization and SSA-assisted clustering

The analysis of residential electricity consumption behavior (RECB) aims to deeply reveal the electricity consumption characteristics of customers that can be used to improve the electricity service performances from the large volume of load data. For analyzing the RECB, various clustering-based methods have been developed. However, with the dimension of power consumption data increasing, it is difficult for traditional methods to accurately identify users’ power consumption patterns. To this end, this paper proposes a novel analysis method for RECB with high-dimensional data based on uniform manifold approximation and projection-criteria importance through intercriteria correlation (UMAP-CRITIC) feature optimization and sparrow search algorithm (SSA)-assisted clustering. Specifically, the high-dimensional raw data combined with load characteristics indexes are first utilized to generate low-dimensional features by UMAP-based dimension reduction theory. Secondly, the contribution weight of each reduced feature to the intrinsic information of the original data is optimized based on the CRITIC weight method, and then the data feature set of RECB identification considering feature contributions is constructed. Finally, the SSA-based k-means is employed to obtain and analyze the various RECBs. The simulation results show that the proposed method can effectively identify high-dimensional electricity consumption data and precisely extract the electricity consumption information of residents

A systematic review and meta-analysis of inflammatory biomarkers in Parkinson’s disease

Abstract Neuroinflammation plays a crucial role in the pathogenesis of Parkinson’s disease (PD), but controversies persist. Studies reporting concentrations of blood or cerebrospinal fluid (CSF) markers for patients with PD and controls were included and extracted. Pooled Hedges’g was adopted to illustrate comparisons, and covariates were used to explore sources of heterogeneity. Finally, 152 studies were included. Increased IL-6, TNF-α, IL-1β, STNFR1, CRP, CCL2, CX3CL1, and CXCL12 levels and decreased INF-γ and IL-4 levels were noted in the PD group. In addition, increased CSF levels of IL-6, TNF-α, IL-1β, CRP and CCL2 were revealed in patients with PD compared to controls. Consequently, significantly altered levels of inflammatory markers were verified between PD group and control, suggesting that PD is accompanied by inflammatory responses in both the peripheral blood and CSF. This study was registered with PROSPERO, CRD42022349182

Additional file 3: of Detection and genetic characterization of feline bocavirus in Northeast China

A maximum-likelihood phylogenetic tree based on partial NS1 gene (705 nt) of feline bocavirus. The black triangles indicate strains belonging to FBoV-1 identified in this study, and the black squares indicate strains belonging to FBoV-2 identified in the present study. (TIF 635 kb

Additional file 2: of Detection and genetic characterization of feline bocavirus in Northeast China

Summary of detailed clinical informations for the 51 FBoV-positive samples identified in the present study. M, male; FM, female; PVC, private veterinary clinic; ASC, animal shelter center. (DOC 98 kb

Additional file 1: of Detection and genetic characterization of feline bocavirus in Northeast China

List of representative bocavirus strains obtained from the GenBank database for genetic analysis and phylogenetic tree construction. (DOC 64 kb

Additional file 4: of Detection and genetic characterization of feline bocavirus in Northeast China

Comparison of nucleotide and amino acid sequence identity in the complete genome and three ORFs of different feline bocavirus genotypes. Nucleotide similarity (%)/ deduced amino acid similarity (%) is shown in the table. Six strains identified in this study were compared to each other and were compared with other reference strains of different FBoV genotypes based on the complete genome and three ORFs. Bold text indicates that the similarity between identified strains and reference strains is higher than 85%. (DOC 62 kb

Additional file 5: of Detection and genetic characterization of feline bocavirus in Northeast China

Phylogenetic tree based on the complete genome of bocaviruses. The phylogenetic analysis was performed using maximum-likelihood method in MEGA 7.0 software. The black triangles and black squares indicate FBoV-1 strains and FBoV-2 strains, respectively, identified in this study. (TIF 1023 kb