Exploring determinants of attraction and helpfulness of online product review:a consumer behaviour perspective

To assist filtering and sorting massive review messages, this paper attempts to examine the determinants of review attraction and helpfulness. Our analysis divides consumers’ reading process into “notice stage” and “comprehend stage” and considers the impact of “explicit information” and “implicit information” of review attraction and review helpfulness. 633 online product reviews were collected from Amazon China. A mixed-method approach is employed to test the conceptual model proposed for examining the influencing factors of review attraction and helpfulness. The empirical results show that reviews with negative extremity, more words, and higher reviewer rank easily gain more attraction and reviews with negative extremity, higher reviewer rank, mixed subjective property, and mixed sentiment seem to be more helpful. The research findings provide some important insights, which will help online businesses to encourage consumers to write good quality reviews and take more active actions to maximise the value of online reviews

Analysis of early efficacy and safety of endovascular therapy for acute mild ischemic stroke with large vessel occlusion

Objective·To investigate the early efficiency and safety of endovascular therapy (EVT) for patients with acute mild ischemic stroke with large vessel occlusion (LVO-MIS).Methods·A total of 31 patients with LVO-MIS who received emergency EVT-assisted standard medical treatment at the Green Channel of Stroke in Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine from June 2016 to October 2022 were retrospectively included as endovascular therapy group (EVT group), and 32 LVO-MIS patients who only received standard medical treatment in the same period were selected as the control group. General clinical data and parameters related to EVT of the two groups were collected. The primary outcome was early efficacy, that is, the NIHSS at seventh day after treatment (d7NIHSS) score decreased by ≥3 points or directly to 0 points from baseline NIHSS score. Secondary outcomes included successful revascularization of blood vessels and early neurological deterioration (END), and safety outcomes included symptomatic intracranial hemorrhage (sICH) and mortality. The primary and secondary outcomes of the two groups of patients were analyzed to evaluate the early efficiency of EVT, and the safety evaluation indicators of the two groups of patients were analyzed to evaluate the safety of EVT. Kruskal-Wallis H test was used to analyze the NIHSS scores of 24 patients in the EVT group who underwent EVT before and after treatment.Results·There was no statistically significant difference in the general clinical data between the two groups, as well as parameters related to EVT such as occlusion site, and onset-to-admission time. The baseline NIHSS score of the EVT group [5.0 (3.0, 5.0) points] was higher than that of the control group [3.5 (2.0, 5.0) points] (P=0.001), and their d7NIHSS score [1.0 (0, 3.0) points] was lower than that of the control group [2.0 (1.0, 5.8) points] (P=0.040). A total of 24 patients (38.1%) in the two groups achieved early efficacy, including 16 cases in the EVT group and 8 cases in the control group; and the early efficacy rate of the EVT group was higher than that of the control group (χ2=4.729, P=0.030). The END rate in the EVT group was lower than that in the control group (χ2=6.097, P=0.014), and there were 29 cases (93.5%) in the EVT group of patients whose blood vessels were successfully reopened. There was no statistically significant difference in sICH rate and mortality rate between the two groups. In the EVT group, there was a statistically significant difference (H=16.997, P=0.000) among the baseline NIHSS scores [5.0 (3.0, 5.0) points] of 24 patients, postoperative 24hNIHSS score [2.0 (0.3, 3.8) points] and d7NIHSS scores [1.0 (0, 2.8) points].Conclusion·EVT is safe and effective in treating LVO-MIS, and the early efficacy rate of EVT is superior to standard medicine treatment, with a lower rate of END and no increased risk of sICH

Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

In mammals, early organogenesis begins soon after gastrulation, accompanied by specification of various type of progenitor/precusor cells. In order to reveal dynamic chromatin landscape of precursor cells and decipher the underlying molecular mechanism driving early mouse organogenesis, we performed single-cell ATAC-seq of E8.5-E10.5 mouse embryos. We profiled a total of 101,599 single cells and identified 41 specific cell types at these stages. Besides, by performing integrated analysis of scATAC-seq and public scRNA-seq data, we identified the critical cis-regulatory elements and key transcription factors which drving development of spinal cord and somitogenesis. Furthermore, we intersected accessible peaks with human diseases/traits-related loci and found potential clinical associated single nucleotide variants (SNPs). Overall, our work provides a fundamental source for understanding cell fate determination and revealing the underlying mechanism during postimplantation embryonic development, and expand our knowledge of pathology for human developmental malformations

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell-cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs.We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200).S

Effects of predictive nursing intervention on cognitive impairment and neurological function in ischemic stroke patients

Abstract Background Ischemic stroke is a clinical emergency caused by insufficient intracranial blood supply, which eventually leads to brain tissue necrosis and neurological impairment. Predictive nursing intervention has achieved impressive success in the nursing of multiple surgeries. However, the role of predictive nursing intervention in the care of patients with ischemic stroke remains unclear. Methods This study was a randomized controlled trial. Based on the inclusion and exclusion criteria, 126 patients were randomly assigned into two groups, namely the control group and the predictive nursing intervention group. Both groups were treated with thrombolytic therapy with alteplase. The patients in the control group were given routine nursing intervention and the predictive nursing intervention group received additional predictive care. Neurologic functions and cognitive impairment were evaluated by National Institutes of Health Stroke Scale (NIHSS), Fugl‐Meyer assessment (FMA), Montreal cognitive assessment (MoCA), and mini‐mental state examination (MMSE) scales, respectively. Door‐to‐Needle Times, venous thromboembolism (VTE)‐related parameters, and complications were recorded. Results Predictive nursing intervention significantly shortened the Door‐to‐Needle Times and enhanced the peak/average femoral venous blood flow and femoral venous diameter. In addition, predictive nursing intervention improved the NIHSS, FMA, MMSE, and MoCA scores and remarkably reduced the recurrence of ischemic stroke, deep vein thrombosis and gingival bleeding. Conclusion Predictive nursing intervention is beneficial to improve the effects of thrombolytic therapy in patients with ischemic stroke, which improves the neurological, cognitive and motor functions of patients, and reduces the occurrence of complications, suggesting an important clinical application value

Identification of the Protective Role of DJ‑1 in Hypoglycemic Astrocyte Injury Using Proteomics

As a common complication of glycemic control in patients with diabetes, hypoglycemia often leads to brain dysfunction or damage. To identify new mechanisms underlying hypoglycemic brain injury, we determined the difference of protein expression profiles in brains between hypoglycemic rats and sham hypoglycemic controls by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. Among the 89 deregulated proteins, DJ-1 protein (Park7) was verified to be upregulated following hypoglycemia insult in vivo and glucose deprivation in an astrocyte cell line (CTX-TNA2) cultured in vitro. Further studies indicated the pro-survival role of autophagy activation and impaired autophagy flux in CTX-TNA2 cells short of glucose. DJ-1 knockdown hindered the initiation of the autophagy process via the AMPK/mTOR pathway and aggravated cell death induced by glucose deficiency. Taken together, our results show that responsive overexpression of DJ-1 plays a protective role against hypoglycemic astrocyte injury partly mediated by the regulation of autophagy

Identification of the Protective Role of DJ‑1 in Hypoglycemic Astrocyte Injury Using Proteomics

As a common complication of glycemic control in patients with diabetes, hypoglycemia often leads to brain dysfunction or damage. To identify new mechanisms underlying hypoglycemic brain injury, we determined the difference of protein expression profiles in brains between hypoglycemic rats and sham hypoglycemic controls by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. Among the 89 deregulated proteins, DJ-1 protein (Park7) was verified to be upregulated following hypoglycemia insult in vivo and glucose deprivation in an astrocyte cell line (CTX-TNA2) cultured in vitro. Further studies indicated the pro-survival role of autophagy activation and impaired autophagy flux in CTX-TNA2 cells short of glucose. DJ-1 knockdown hindered the initiation of the autophagy process via the AMPK/mTOR pathway and aggravated cell death induced by glucose deficiency. Taken together, our results show that responsive overexpression of DJ-1 plays a protective role against hypoglycemic astrocyte injury partly mediated by the regulation of autophagy

Aquaporin-4 deletion ameliorates hypoglycemia-induced BBB permeability by inhibiting inflammatory responses

Abstract Background Severe hypoglycemia induces brain edema by upregulating aquaporin-4 (AQP4) expression and by degrading tight junctions. Acute severe hypoglycemia induces a proinflammatory environment that may contribute to a disruption in the epithelial barrier by decreasing tight junction protein expression. Interestingly, the altered AQP4 expression has been considered to play a critical role in neuroinflammation during acute brain injury. It has been shown that AQP4 deletion reduces brain inflammation in AQP4-null mice after intracerebral LPS injection. However, the effect of AQP4 deletion regarding protection against hypoglycemia-induced blood-brain barrier (BBB) breakdown is unknown. Methods An acute severe hypoglycemic stress model was established via injection of 4 unit/kg body weight of insulin. Evans blue (EB) staining and water measurement were used to assess BBB permeability. Western blot, reverse transcription polymerase chain reaction, and immunofluorescence were used to detect the expression of related proteins. The production of cytokines was assessed via enzyme-linked immunosorbent assay. Results Hypoglycemia-induced brain edema and BBB leakage were reduced in AQP4−/− mice. AQP4 deletion upregulated PPAR-γ and inhibited proinflammatory responses. Moreover, knockdown of aquaporin-4 by small interfering RNA in astrocytes co-cultured with endothelial cells effectively reduced transendothelial permeability and degradation of tight junctions. Treatment with PPAR-γ inhibitors showed that upregulation of PPAR-γ was responsible for the protective effect of AQP4 deletion under hypoglycemic conditions. Conclusions Our data suggest that AQP4 deletion protects BBB integrity by reducing inflammatory responses due to the upregulation of PPAR-γ expression and attenuation of proinflammatory cytokine release. Reduction in AQP4 may be protective in acute severe hypoglycemia