Study on Coupling and Coordination Relationship between Urbanization and Ecosystem Service Value in Jiangsu Province, China

Urbanization has a significant negative impact on both the structure and function of ecosystems, as it is a major part of the human-caused transformation of natural landscapes. Concurrently, the attenuation of ecosystem service values (ESVs) poses critical impediments to urbanization and imperils human well-being. Investigating the interactive coupling and coordination relationship between urbanization and ESV is paramount in informing urban development strategies and environmental preservation efforts. Using Jiangsu Province as a representative case, this study forges an urbanization assessment index framework, estimates ESV, and subsequently delves into the multifaceted nexus between urbanization and ESV. The findings disclose a gradual uptick in urbanization levels in Jiangsu Province, underscored by conspicuous regional disparities typified in the subregions of southern Jiangsu, central Jiangsu, and northern Jiangsu, mirroring the high congruence observed in the economic urbanization subsystem. However, this upward trajectory in urbanization coincides with an overarching descent in ESV, with the most pronounced declines manifesting in regions characterized by elevated urbanization levels, such as Nantong and Suzhou. A robust interrelationship between urbanization and ESV is discernible throughout Jiangsu Province. Nevertheless, certain cities exhibit perturbations and retrogression in the associations between urbanization subsystems and ESV. The coupling coordination between population urbanization and ESV is characterized by disharmony while the coordination of economic urbanization markedly lags. Additionally, several cities are witnessing a progressive deterioration in the coordination relationships between ESV subsystems (food production, soil conservation, gas regulation, and raw material production) and urbanization. In light of these findings, it is recommended that governmental authorities enact measures to harmonize urban development with environmental preservation, safeguard the integrity of ecosystem functions, and facilitate the sustainable management of land resources

Spatiotemporal evolution and relationship between construction land expansion and territorial space conflicts at the county level in Jiangsu Province

In rapidly urbanizing areas, intense human development activities and fierce competition for spatial resources have led to apparent territorial space conflicts. This paper selects Jiangsu Province as the study area to explore the spatiotemporal evolution characteristics and response relationship between the expansion of county-level construction land and territorial space conflicts from 1985 to 2020 to provide a reference for regional territorial space optimization. The results show that the construction land expansion intensity (CEI) at the county level showed a spatial characteristic of greater power in the southeast than in the northwest. The spatial expansion mode (SEI) was mainly characterized by spread type, with construction land experiencing marginal expansion, the emergence of new independent expansion points, and then the expansion process of agglomeration distribution. From 1985 to 2020, there was a significant increase in territorial space conflicts and an increase in spatial aggregation at the county level. The regions with high space conflicts intensity index (SCII) values and high increase in space conflicts intensity changes (SCIIC) were mainly located in Xuzhou, Nanjing, and at the junction of Changzhou, Wuxi, Suzhou, and Zhenjiang. There was a positive correlation between CEI and SCIIC, and the correlation of positive correlation units increased from south to north. There was a positive correlation between SEI and SCIIC, and the units with a strong positive correlation were mainly located in Xuzhou, Nantong, Changzhou, and Wuxi. The changes in population density and GDP also affect SCIIC. This paper strengthens the understanding of the spatiotemporal evolution characteristics and related relationships of construction land expansion and territorial space conflicts at the county level over a long time, which can provide a decision-making basis and reference for the regulation of county construction land expansion, territorial space optimization in Jiangsu Province

Mesenchymal stem cells microvesicles stabilize endothelial barrier function partly mediated by hepatocyte growth factor (HGF)

Abstract Background Mesenchymal stem cells microvesicles (MSC-MVs) stabilize endothelial barrier function in acute lung injury (ALI); however, the detailed mechanism remains to be further defined. Hepatocyte growth factor (HGF), which is derived from MSC-MVs, might have a key role in the restoration of endothelial barrier function by MSC-MVs. Methods MSCs with lentiviral vector-mediated HGF gene knockdown (siHGF-MSC) were generated. A co-culture model of pulmonary microvascular endothelial cells and MSC-MVs collected from MSCs or siHGF-MSCs after 24 h of hypoxic culture was utilized. Then, endothelial paracellular and transcellular permeabilities were detected. VE-cadherin, and occludin protein expression in the endothelial cells was measured using Western blot. Endothelial cell proliferation was analysed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Endothelial cell apoptosis was analysed using TUNEL assay. Finally, IL-6 and IL-10 production was determined via an enzyme-linked immunosorbent assay (ELISA). Results Treatment with MSC-MVs significantly decreased LPS-induced endothelial paracellular and transcellular permeabilities, and the effect was significantly inhibited after HGF gene knockdown in MSC-MVs. Furthermore, treatment with MSC-MVs increased the expression of the endothelial intercellular junction proteins VE-cadherin and occludin. Treatment with MSC-MVs also decreased endothelial apoptosis and induced endothelial cell proliferation. Finally, the treatment reduced IL-6 production and increased IL-10 production in the conditioned media of endothelial cells. However, the effects of the treatment with MSC-MVs were inhibited after HGF gene knockdown. Conclusions MSC-MVs protect the barrier functions of pulmonary microvascular endothelial cells, which can be partly attributed to the presence of HGF in the MSC-MVs

A nomogram based on TFE3 IHC results and clinical factors as a preliminary screening scheme for TFE3‐rearranged renal cell carcinoma

Abstract Background TFE3 immunohistochemistry (TFE3‐IHC) is controversial in the diagnosis of TFE3‐rearranged renal cell carcinoma (TFE3‐rearranged RCC). This study is to investigate the accuracy and sensitivity of IHC and establish a predictive model to diagnose TFE3‐rearranged RCC. Methods Retrospective analysis was performed by collecting IHC and fluorescence in situ hybridization (FISH) results from 228 patients. IHC results were evaluated using three scoring systems. Scoring system 1 is graded based on nuclear staining intensity, scoring system 2 is graded based on the percentage of stained tumor cell nuclei, and scoring system 3 is graded based on both the nuclear staining intensity and the percentage. We collected patients' IHC results and clinical information. Important variables were screened based on univariate logistic regression analysis. Then, independent risk factors were established through multivariate logistic regression, and a nomogram model was constructed. The model was validated in internal test set and external validation set. The receiver operating characteristic curve (ROC curve), calibration curve, and decision curve analysis (DCA) were generated to assess discriminative ability of the model. Results The accuracy of IHC based on three scoring systems were 0.829, 0.772, and 0.807, respectively. The model included four factors including age, gender, lymph node metastasis and IHC results. Area under the curve (AUC) values were 0.935 for the training set, 0.934 for the internal test set, 0.933 for all 228 patients, and 0.916 for the external validation set. Conclusions TFE3 IHC has high accuracy in the diagnosis of TFE3‐rearranged RCC. Clinical information such as age and lymph node metastasis are independent risk factors, which can be used as a supplement to the results of TFE3 IHC. This study confirms the value of IHC in the diagnosis of TFE3‐rearranged RCC. The accuracy of the diagnosis can be improved by incorporating IHC with other clinical risk factors

Hsa_circ_0057105 modulates a balance of epithelial‐mesenchymal transition and ferroptosis vulnerability in renal cell carcinoma

Abstract Background The incidence of renal cell carcinoma (RCC) has increased in recent years. Metastatic RCC is common and remains a major cause of mortality. A regulatory role for circular RNAs (circRNAs) in the occurrence and progression of RCC has been identified, but their function, molecular mechanisms, and potential clinical applications remain poorly understood. Methods High‐throughput RNA sequencing was used to explore the differential expression of circRNAs and their related pathways in RCC patients. Transwell and CCK‐8 assays were used to assess the function of hsa_circ_0057105 in RCC cells. The clinical relevance of hsa_circ_0057105 was evaluated in a cohort of RCC patients. The hsa_circ_0057105 regulatory axis was defined using RNA pull‐down, luciferase reporter assays, and fluorescence in situ hybridization assays, and the in vivo effect of hsa_circ_0057105 was validated using animal experiments. Results Single‐sample gene set enrichment analysis and correlation analysis of RNA‐seq data showed that hsa_circ_0057105 was potentially oncogenic and may serve to regulate epithelial‐mesenchymal transition (EMT) activation in RCC. Hsa_circ_0057105 expression was associated with advanced TNM stages and was an independent prognostic factor for poor RCC patient survival. Phenotypic studies show that hsa_circ_0057105 can enhance the migration and invasion abilities of RCC cells. Further, hsa_circ_0057105 was shown to inhibit the expression of miR‐577, a miRNA that regulated the expression of both COL1A1, which induced EMT activation, and VDAC2, which modulated ferroptosis sensitivity. The dual regulatory roles of hsa_circ_0057105 on EMT and ferroptosis sensitivity were verified using rescue experiments. Animal studies confirmed that hsa_circ_0057105 increased the metastatic ability and ferroptosis sensitivity of RCC cells in vivo. Conclusions In RCC, hsa_circ_0057105 regulates COL1A1 and VDAC2 expression through its sponge effect on miR‐577, acting like a ‘double‐edged sword’. These findings provide new insight into the relationship between EMT and ferroptosis in RCC and provide potential biomarkers for RCC surveillance and treatment

Electrostatic force-driven oxide heteroepitaxy for interface control

Oxide heterostructure interfaces create a platform to induce intriguing electric and magnetic functionalities for possible future devices. A general approach to control growth and interface structure of oxide heterostructures will offer a great opportunity for understanding and manipulating the functionalities. Here, it is reported that an electrostatic force, originating from a polar ferroelectric surface, can be used to drive oxide heteroepitaxy, giving rise to an atomically sharp and coherent interface by using a low-temperature solution method. These heterostructures adopt a fascinating selective growth, and show a saturation thickness and the reconstructed interface with concentrated charges accumulation. The ferroelectric polarization screening, developing from a solid-liquid interface to the heterostructure interface, is decisive for the specific growth. At the interface, a charge transfer and accumulation take place for electrical compensation. The facile approach presented here can be extremely useful for controlling oxide heteroepitaxy and producing intriguing interface functionality via electrostatic engineering