ヘキサホモトリオキサカリックスアレーン オ キバン ト スル シンキ ケイコウセイ カガク センサーノ ゴウセイ トヒョウカ ニ カンスル ケンキュウ

博士（工学）佐賀大

Solvent effect and fluorescence response of the 7-tert-butylpyrene-dipicolylamine linkage for the selective and sensitive response toward Zn(II) and Cd(II) ions

The different binding behaviour of 7-tert-butylpyrene based chemosensors bearing dipicolylamine (Dpa) linkages at the 1,3-positions was investigated in various solvents for the sensing of Zn(II) and Cd(II).The potential mono-chelating ligand L1 follows the same binding pattern in both THF and methanol–water solvent systems, exhibiting higher selectivity and sensitivity for Cd(II) over Zn(II) mainly in THF solvent system. The potential bis-chelate ligand L2 can selectively bind both Zn(II) and Cd(II) in a 1:1 ratio in THF, whereas in methanol–water (7:3) at pH = 7.0; a 1:2 binding ratio was observed. In THF, two sites of ligand L2 can only selectively and sensitively bind one Zn(II) or Cd(II). The different complexation behaviour of L1 and L2 in different solvents were studied by means of fluorescence spectra and ¹H-NMR titration experiments in the presence of Zn(II) and Cd(II)

A pyrene-armed hexahomotrioxacalix[3]arene as a multi-sensor via synergistic and demetallation effects

A new pyrene-armed hexahomotrioxacalix[3]arene L has been synthesized, which exhibits a pronounced fluorescence enhancement response toward Cu²⁺ ions via a Zn²⁺ or Cd²⁺ triggered synergistic effect. Additionally, the L·Cu²⁺+ complex can subsequently serve as a sensor for F⁻ via anion-induced demetallation. The fluorescence responses by the input of Cu²⁺, Zn²⁺/Cd²⁺ and F⁻ can be constructed as a combinational logic gate which mimics a set of molecular traffic signals

Fluorescent turn-on sensors based on pyrene-containing Schiff base derivatives for Cu2+ recognition: spectroscopic and DFT computational studies

A new fluorescent chemosensor L1, pyrene containing long chain Schiff base derivative in 1-position has been synthesized. Similarly, the receptors L2 and L3 are also designed in order to compare the binding ability for detection of Cu2+. The receptors exhibit very weak fluorescence (Φ = 0.01) due to the photoinduced electron transfer (PET). Upon addition of 10 equiv. of Cu2+, the emission intensity of ligands L1 and L2 are increased 65-fold (Φ = 0.31) and 25-fold (Φ = 0.08) in CH3CN/CH2Cl2 solvents system respectively. NMR titration experiments, spectroscopic and DFT computational studies confirmed the binding phenomena and sensitivity of Cu2+

Mitigation of premature ovarian failure by over-expression of lentivirus vector-mediated Wilms tumor-suppressor gene

Purpose: To investigate the effect of WT1(Wilms tumor-suppressor gene) overexpression on premature ovarian failure (POF)-mediated ovarian dysfunction.Methods: Three mice groups were used: control group (untreated mice), POF group (mice sterilized by intravenous injection of cyclophosphamide and busulfan), and POF-LV(lentiviral vector)GFP(green fluorescent protein)WT1 group (POF mice given intra-ovarian microinjection of LVGFPWT1, a WT1overexpressing lentiviral vector, one week after sterilization). Real time-PCR was employed to analyze in vitro WT1 overexpression levels. Overall ovarian function was measured by hormonal assay, H & E staining, and immuno-histochemical techniques.Results: Overexpression of WT1 in mice models of POF alleviated ovarian granulosa cell (GC) damage, increased ovary weight, and significantly increased follicular number (p < 0.05). Radioimmunoassays revealed reduction in plasma estradiol (E2) and follicle-stimulating hormone (FSH, p < 0.05). However, results from immune-histochemical assays showed reduced Bax expression levels, and increased expression of Bcl-2 in WTI-overexpression mice, relative to POF mice.Conclusion: Overexpression of WT1 may stimulate repair of ovarian tissue while improving endocrine function by inhibiting ovarian cell apoptosis signaling pathway in POF mice.Keywords: Premature ovarian failure, Wilms tumor suppressor gene, Chemotherapy, Apoptosis, Estradio

Studies on Synthesis and Evaluation of Novel Ratiometric Fluorescent Chemosensors Based on Hexahomotrioxacalix[3]arenes

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Pipe pressure of mining wet shot crete flowing in pipes

In order to explore the pressure change law of mining wet shotcrete in pipes, the rheological model was built based on rheology principle, and the computational formula of rheological parameters of wet shotcrete was deduced with the linear regression. 100 m full-scale pipeline platform of wet shotcrete was designed and built to study the relationship of pressure and other factors including flow rate, water cement ratio, mix proportion, and pipe bends. Results show: pipe pressure increases with the increase in flow rate and declines with the increase in water-cement ratio, the pressure may fluctuate with a high water cement ratio which can cause cement overhydration and bleeding separation. It will be more beneficial to transport materials if the continuous grading and straight pipe were considered. According to the tests of mix proportion 1:1.5:2.25, the pressure drop is 0.032 MPa·m−1 and the bend pressure drop is 1.3 times higher than in the straight line. We also conclude that solid phase pressure is bigger than liquid phase pressure and they both decline along the pipe based on FLUENT simulation. Finally, the formula of on-way resistance used in mine production was deduced

On the Performance of a Capacitively Coupled Electrical Impedance Tomography Sensor with Different Configurations

Capacitively coupled electrical impedance tomography (CCEIT) is a new kind of electrical resistance tomography (ERT) which realizes contactless measurement by capacitive coupling and extends traditional resistance measurement to total impedance measurement. This work investigates the performance of a CCEIT sensor with three different configurations, including the unshielded configuration, the shielded configuration A (the CCEIT sensor with the external shield) and the shielded configuration B (the CCEIT sensor with both the external shield and the radial screens). The equivalent circuit models of the measurement electrode pair of the CCEIT sensor with different configurations were developed. Additionally, three CCEIT prototypes corresponding to the three configurations were developed. Both the simulation work and experiments were carried out to compare various aspects of the three CCEIT prototypes, including the sensitivity distribution, the impedance measurement and the practical imaging performance. Simulation results show that shielded configurations improve the overall average sensitivity of the sensitivity distributions. Shielded configuration A contributes to improve the uniformity of the sensitivity distributions, while shielded configuration B reduces the uniformity in most cases. Experimental results show that the shielded configurations have no significant influence on the imaging quality of the real part of impedance measurement, but do make sense in improving the imaging performance of the imaginary part and the amplitude of impedance measurement. However, configuration B (with radial screens) has no significant advantage over configuration A (without radial screens). This work provides an insight into how shielding measures influence the performance of the CCEIT sensor, in addition to playing an important role in shielding unwanted noise and disturbances. The research results can provide a useful reference for further development of CCEIT sensors

Erratum: Liu, Y.-Z.; Jiang, X.-K.; Cao, W.-F.; Sun, J.-Y.; Gao, F. Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers. <em>Sensors</em> 2018, <em>18</em>, 589

The authors wish to make the following corrections to their paper [...

Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers

Carboxyl-functionalized semiconducting polymer dots (Pdots) were synthesized as an energy donor by the nanoprecipitation method. A black hole quenching dye (BHQ-labelled thrombin aptamers) was used as the energy acceptor, and fluorescence resonance energy transfer between the aptamers and Pdots was used for fluorescence quenching of the Pdots. The addition of thrombin restored the fluorescence intensity. Under the optimized experimental conditions, the fluorescence of the system was restored to the maximum when the concentration of thrombin reached 130 nM, with a linear range of 0–50 nM (R2 = 0.990) and a detection limit of 0.33 nM. This sensor was less disturbed by impurities, showing good specificity and signal response to thrombin, with good application in actual samples. The detection of human serum showed good linearity in the range of 0–30 nM (R2 = 0.997), with a detection limit of 0.56 nM and a recovery rate of 96.2–104.1%, indicating that this fluorescence sensor can be used for the detection of thrombin content in human serum