Regulation of MntH by a Dual Mn(II)- and Fe(II)-Dependent Transcriptional Repressor (DR2539) in Deinococcus radiodurans

The high intracellular Mn/Fe ratio observed within the bacteria Deinococcus radiodurans may contribute to its remarkable resistance to environmental stresses. We isolated DR2539, a novel regulator of intracellular Mn/Fe homeostasis in D. radiodurans. Electrophoretic gel mobility shift assays (EMSAs) revealed that DR2539 binds specifically to the promoter of the manganese acquisition transporter (MntH) gene, and that DR0865, the only Fur homologue in D. radiodurans, cannot bind to the promoter of mntH, but it can bind to the promoter of another manganese acquisition transporter, MntABC. β-galactosidase expression analysis indicated that DR2539 acts as a manganese- and iron-dependent transcriptional repressor. Further sequence alignment analysis revealed that DR2539 has evolved some special characteristics. Site-directed mutagenesis suggested that His98 plays an important role in the activities of DR2539, and further protein-DNA binding activity assays showed that the activity of H98Y mutants decreased dramatically relative to wild type DR2539. Our study suggests that D. radiodurans has evolved a very efficient manganese regulation mechanism that involves its high intracellular Mn/Fe ratio and permits resistance to extreme conditions

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Co-benefits of reducing CO2 and air pollutant emissions in the urban transport sector: A case of Guangzhou

The transport sector has become an important source of urban greenhouse gas (GHG) and air pollutant emissions, with the continuous growth of transportation demand. The co-benefits of reducing GHG and air pollutant emissions in the transport sector are receiving more and more attention. Taking Guangzhou as the case study city, this study quantitatively analyzed the co-benefits of reducing CO2 and air pollutant emissions under the sustainable scenario in the transport sector by developing a quantitative analysis model based on the Long-range Energy Alternatives Planning (LEAP) framework. The co-benefits quantitative parameter and the co-benefits radar chart analysis method are used to identify and evaluate the generated co-benefits, and then the co-benefits generated by implementing the sustainable measures are discussed. The results show that adjusting transport modes and increasing the application of electricity are the two most effective measures for achieving the co-benefits of reducing CO2 and air pollutant emissions in the Guangzhou transport sector. Thus, these two measures are highly preferential for mitigating CO2 and air pollutant emissions. In addition, increasing the application of biofuel and hydrogen energy also has good co-benefits. However, increasing the application of Liquefied Natural Gas (LNG) has poor co-benefits. Promoting the utilization of LNG intercity buses, trucks, and cargo ships will lead to emission increases of CO and HC compared to the current policy scenario, and small emission reductions of CO2 and other air pollutants. Therefore, to promote the utilization of LNG vehicles and ships, it is necessary to cooperate with the use of air pollutant end removal devices and high-pressure direct injection (HPDI) gas engines. (C) 2020 International Energy Initiative. Published by Elsevier Inc. All rights reserved

Tri-SIFT: A Triangulation-Based Detection and Matching Algorithm for Fish-Eye Images

Keypoint matching is of fundamental importance in computer vision applications. Fish-eye lenses are convenient in such applications that involve a very wide angle of view. However, their use has been limited by the lack of an effective matching algorithm. The Scale Invariant Feature Transform (SIFT) algorithm is an important technique in computer vision to detect and describe local features in images. Thus, we present a Tri-SIFT algorithm, which has a set of modifications to the SIFT algorithm that improve the descriptor accuracy and matching performance for fish-eye images, while preserving its original robustness to scale and rotation. After the keypoint detection of the SIFT algorithm is completed, the points in and around the keypoints are back-projected to a unit sphere following a fish-eye camera model. To simplify the calculation in which the image is on the sphere, the form of descriptor is based on the modification of the Gradient Location and Orientation Histogram (GLOH). In addition, to improve the invariance to the scale and the rotation in fish-eye images, the gradient magnitudes are replaced by the area of the surface, and the orientation is calculated on the sphere. Extensive experiments demonstrate that the performance of our modified algorithms outweigh that of SIFT and other related algorithms for fish-eye images

Repression of recA Induction by RecX Is Independent of the RecA Protein in Deinococcus radiodurans▿

Besides inhibiting RecA activity at the protein level, Deinococcus radiodurans RecX can suppress RecA induction at the transcriptional level. The regulation of RecX on recA induction is independent of RecA activity, and its N terminus is involved in this process

Effect of Chromium on Microstructure and Oxidation Wear Behavior of High-Boron High-Speed Steel at Elevated Temperatures

In order to investigate the effect of Cr content on the microstructures and oxidation wear properties of high-boron high-speed steel (HBHSS), so as to explore oxidation wear resistant materials (e.g., hot rollers), a scanning electron microscope, an X-ray diffractometer, an electron probe X-ray microanalysis and an oxidation wear test at elevated temperatures were employed to investigate worn surfaces and worn layers. The results showed that the addition of Cr resulted in the transformation of martensite into ferrite and pearlite, while the size of the grid morphology of borides in HBHSSs was refined. After oxidation wear, oxide scales were formed and the high-temperature oxidation wear resistance of HBHSSs was gradually improved with increased additions of Cr. Meanwhile, an interaction between temperature and load in HBHSSs during oxidation wear occurred, and the temperature had more influence on the oxidation wear properties of HBHSSs. SEM observations indicated that a uniform and compact oxide film of HBHSSs in the worn surface at elevated temperatures was generated on the worn surface, and the addition of Cr also reduced the thickness of oxides and inhibited the spallation of worn layers, which was attributed to improvements in microhardness and oxidation resistance of the matrix in HBHSSs. A synergistic effect of temperature and load in HBHSSs with various Cr additions may dominate the oxidation wear process and the formation and spallation of oxide films

Extracellular dGMP enhances Deinococcus radiodurans tolerance to oxidative stress.

Free extracellular DNA provides nutrition to bacteria and promotes bacterial evolution by inducing excessive mutagenesis of the genome. To understand the influence of extracellular DNA fragments on D. radiodurans, we investigated cell growth and survival after extracellular DNA or dNMPs treatment. The results showed that the extracellular DNA fragments inhibited the growth of D. radiodurans. Interestingly, dGMP, a DNA component, enhanced D. radiodurans tolerance to H(2)O(2) and gamma-radiation significantly. Further experiments indicated that extracellular dGMP stimulated the activity of one catalase (KatA, DR1998), and induced gene transcription including the extracellular nuclease (drb0067). When this only extracellular nuclease gene (drb0067) in D. radiodurans was deleted, the mutant strain showed more sensitive to H(2)O(2) and gamma-radiation than the wild type strain. These results suggest that DRB0067 plays an important role in oxidative stress resistance. Taken together, we proposed a new anti-oxidation mechanism in D. radiodurans. This mechanism acts to increase expression levels of DRB0067 which then secretes active nuclease to degrade extracellular DNA fragments. The extracellular nuclease has a two-fold benefit, creating more free dNTPs for further cell protection and the removal of extracellular DNA fragments

Mn(II) and Fe(II) modulate the binding activity of DR2539 <i>in vivo</i>.

<p>(A) Effects of divalent metals (50 µM) on expression of pRAZH in <i>D. radiodurans</i>. Data shown are the means ± standard deviations of three independent experiments. (B) Effects of Mn(II) (squares) and Fe(II) (circles) on the expression of pRAZH in wild-type samples expressing DR2539. (C) Effects of Mn(II) (squares) and Fe(II) (circles) on the expression of pRAZH in the <i>dr2539</i> null mutant. (D) Rea-time PCR analysis of the <i>dr1709</i> gene expression using <i>dr0089</i> as internal control gene. Longitudinal axes indicate the change fold of <i>dr1709</i> mRNA relative to controls. Control cells were cultured in medium without Mn(II). *, <i>P</i><0.05 relative to control. The data are the means ± standard deviations of three independent experiments.</p