4 research outputs found
The growth curve of the microbes
The growth curve of the microbe
DataSheet1_Controllable AgNPs encapsulation to construct biocompatible and antibacterial titanium implant.PDF
Silver nanoparticles (AgNPs) are progressively becoming an in-demand material for both medical and life use due to their effective antimicrobial properties. The high surface area-to-volume ratio endows AgNPs with enhanced antibacterial capacity accompanied by inevitable cytotoxicity. Surface coating technique could precisely regulate the particle shape, aggregation, and Ag+ release pattern of AgNPs, by which the cytotoxicity could be significantly reduced. Various coating methods have been explored to shell AgNPs, but it remains a great challenge to precisely control the aggregation state of AgNPs and their shell thickness. Herein, we proposed a simple method to prepare a tunable polydopamine (pDA) coating shell on AgNPs just by tuning the reaction pH and temperature, yet we obtained high antibacterial property and excellent biocompatibility. SEM and TEM revealed that pDA coated AgNPs can form core-shell structures with different aggregation states and shell thickness. Both in vitro and in vivo antibacterial tests show that acid condition and heat-treatment lead to appropriate AgNPs cores and pDA shell structures, which endow Ti with sustained antibacterial properties and preferable cell compatibility. One month of implantation in an infected animal model demonstrated that the obtained surface could promote osteogenesis and inhibit inflammation due to its strong antibacterial properties. Therefore, this study provides a promising approach to fabricate biocompatible antibacterial surface.</p
Guidance of Stem Cells to a Target Destination in Vivo by Magnetic Nanoparticles in a Magnetic Field
Stem
cells contribute to physiological processes such as postischemic
neovascularization and vascular re-endothelialization, which help
regenerate myocardial defects or repair vascular injury. However,
therapeutic efficacy of stem cell transplantation is often limited
by inefficient homing of systemically administered cells, which results
in a low number of cells accumulating at sites of pathology. In this
study, anti-CD34 antibody-coated magnetic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>@PEG-CD34) are shown to have high affinity to stem cells.
The results of hemolysis rate and activated partial thromboplastin
time (APTT) tests indicate that such nanoparticle may be used safely
in the blood system. In vitro studies showed that a nanoparticle concentration
of 100 μg/mL gives rise to a significant increase in cell retention
using an applicable permanent magnet, exerting minimal negative effect
on cell viability and migration. Subsequent in vivo studies indicate
that nanopartical can specifically bind stem cells with good magnetic
response. Anti-CD34 antibody coated magnetic nanoparticle may be used
to help deliver stem cells to a lesion site in the body for better
treatment