Genotype data-PONE-D-13-20610

This file includes genotype data of the paper, “Population structure and genetic diversity of native and invasive populations of Solanum rostratum (Solanaceae)." We genotyped 329 individuals at 10 microsatellite loci to determine the levels of genetic diversity and to investigate population structure of native and introduced populations of S. rostratum. We studied five populations in each of three regions across two continents: Mexico, the U.S.A. and China

Venn diagram of the distribution of all distinct alleles across loci for the three geographic regions studied: Mexico, the U.S.A. and China.

<p>Overlapping areas (intersections) denote shared alleles between two or three regions.</p

Unweighted pair group method with arithmetic mean (UPGMA) tree depicting the relationships between 15 <i>Solanum rostratum</i> populations from Mexico, the U.S.A. and China.

<p>The tree is based on the standardized genetic distances among populations (Nei's <i>D</i>_<i>S</i>) calculated from a matrix of genotypes at 10 microsatellite loci. The numbers above the nodes represent percentage of bootstrap support (1000 replications). Only bootstrap support above 50% is shown. Population codes as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#pone-0079807-t001" target="_blank">Table 1</a>.</p

Network diagram of 15 populations of <i>Solanum rostratum</i> in Mexico (green circles), the U.S.A. (blue circles), and China (red circles) calculated in POPULATION GRAPH [32].

<p>The diameter of each sphere is proportional to the amount of heterozygosity in each population. Black lines represent connections (edges) linking two populations.</p

Map showing the location of the 15 sampled populations of <i>Solanum rostratum</i> used in this study.

<p>Left panel: Mexican (native, green circles) and U.S.A. populations from the U.S.A. (residence time >130 years, blue circles). Right panel: Chinese populations (residence time <31 years, red circles). Details of localities and population codes are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#pone-0079807-t001" target="_blank">Table 1</a>.</p

Posterior probability of cluster assignment in nested InStruct analyses of 329 <i>Solanum rostratum</i> individuals genotyped at 10 microsatellite loci across 15 populations in three geographic regions: Mexico, the U.S.A. and China.

<p>Each bar represents a single individual with populations separated by black lines and arranged from North to South within each region. Panel <b>A</b> depicts the uppermost level of population structure across regions. The optimal number of clusters (<i>K*</i> = 2) was calculated using Evanno’s et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#B31" target="_blank">31</a>] <i>∆K</i> statistic. For illustration, panel <b>B</b> shows the assignment probabilities for <i>K</i> = 3. Panel <b>C</b> represent two separate analyses of either U.S.A./Chinese or Mexican populations; the optimal number of clusters [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079807#B31" target="_blank">31</a>] in each of these separate analyses was <i>K</i>_US-C<i>*</i> =<i>K</i>_US-C<i>*</i> = 2. Panel <b>D</b>, shows assignment probabilities for <i>K</i> = 3, for each separate analysis.</p

Additional file 1: of Nutrition assessment of vitamin A and vitamin D in northeast Chinese population based-on SPE/UPLC/PDA

Table S1. TQ MS condition of detecting of serum retinol and 25OHD3. Figure S1. Calibration graphs for retinol and 25OHD3. Figure S2. Plots of the percentage difference in 25OHD3 and retinol concentrations measured in ULPC/PDA by UPLC/MS-MS. (DOCX 125 kb