Cholesterol-Modified Caged siRNAs for Photoregulating Exogenous and Endogenous Gene Expression

siRNA has been widely applied in research and drug development due to its sequence-specific gene silencing ability. However, how to spatiotemporally control its function is still one of its challenges. Light, a fast and noninvasive trigger, is a promising tool for spatiotemporal control of gene expression. Here, we designed and synthesized a new series of caged siRNAs modified with single cholesterol at the 5′ terminal of antisense strand RNA through a photolabile linker (Chol-PL-siRNAs). We demonstrated that these caged siRNAs were successfully used to photochemically regulate both exogenous (<i>firefly luciferase</i> and <i>gfp</i>) and endogenous gene expression (mitotic kinesin-5, <i>Eg5</i>) in cells

Fate of fenoxaprop-ethyl and fenoxaprop

Concentration and SD of fenoxaprop-ethyl and fenoxaprop in control and biochar-amended soils during the incubatio

Vitamin E‑Labeled Polyethylenimine for <i>in vitro</i> and <i>in vivo</i> Gene Delivery

A series of Vitamin E (vitE)-labeled PEIs (PEI-vitE_<i>n</i>) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitE_<i>n</i>/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell lines. PEI-vitE₆ showed the best performance in <i>gfp</i> pDNA delivery and following GFP expression in HEK-293A cells. In addition, <i>in vivo</i> gene delivery in living mice also confirmed that PEI-vitE₆ showed low toxicity and efficiently delivered <i>gfp</i> pDNA to the cells of liver and lung tissues for gene expression

Oxidation-Induced Topological Phase Transition in Monolayer 1T′-WTe₂

Monolayer (ML) tungsten ditelluride (WTe₂) is a well-known quantum spin Hall (QSH) insulator with topologically protected gapless edge states, thus promising dissipationless electronic devices. However, experimental findings exhibit the fast oxidation of ML WTe₂ in ambient conditions. To reveal the changes of topological properties of WTe₂ arising from oxidation, we systematically study the surface oxidation reaction of ML 1T′-WTe₂ using first-principles calculations. The calculated results indicate that the fast oxidation of WTe₂ originates from the existence of H₂O in air, which significantly promotes the oxidation of ML 1T′-WTe₂. More importantly, this low-coverage oxidized WTe₂ loses its topological features and is changed into a trivial insulator. Furthermore, we propose a fully oxidized ML WTe₂ that can still possess the QSH insulator states. The topological phase transition induced by oxidation provides exotic insight into understanding the topological features of layered transition-metal dichalcogenide materials

sj-docx-1-asm-10.1177_10731911241241495 – Supplemental material for Interpersonal Problem Profiles of Personality and Psychopathology Constructs in Chinese Undergraduates and Offenders

Supplemental material, sj-docx-1-asm-10.1177_10731911241241495 for Interpersonal Problem Profiles of Personality and Psychopathology Constructs in Chinese Undergraduates and Offenders by Yuping Liu, Christopher J. Hopwood, Aaron L. Pincus, Bingtao Zhou, Jiali Yang, Shuliang Bai and Bo Yang in Assessment</p