L1\mathcal{L}_1Quad: L1\mathcal{L}_1 Adaptive Augmentation of Geometric Control for Agile Quadrotors with Performance Guarantees

Quadrotors that can operate safely in the presence of imperfect model knowledge and external disturbances are crucial in safety-critical applications. We present L1Quad, a control architecture for quadrotors based on the L1 adaptive control. L1Quad enables safe tubes centered around a desired trajectory that the quadrotor is always guaranteed to remain inside. Our design applies to both the rotational and the translational dynamics of the quadrotor. We lump various types of uncertainties and disturbances as unknown nonlinear (time- and state-dependent) forces and moments. Without assuming or enforcing parametric structures, L1Quad can accurately estimate and compensate for these unknown forces and moments. Extensive experimental results demonstrate that L1Quad is able to significantly outperform baseline controllers under a variety of uncertainties with consistently small tracking errors.Comment: The first two authors contributed equally to this wor

Developing and validating a nomogram for cognitive impairment in the older people based on the NHANES

ObjectiveTo use the United States National Health and Nutrition Examination Study (NHANES) to develop and validate a risk-prediction nomogram for cognitive impairment in people aged over 60 years.MethodsA total of 2,802 participants (aged ≥ 60 years) from NHANES were analyzed. The least absolute shrinkage and selection operator (LASSO) regression model and multivariable logistic regression analysis were used for variable selection and model development. ROC-AUC, calibration curve, and decision curve analysis (DCA) were used to evaluate the nomogram’s performance.ResultsThe nomogram included five predictors, namely sex, moderate activity, taste problem, age, and education. It demonstrated satisfying discrimination with a AUC of 0.744 (95% confidence interval, 0.696–0.791). The nomogram was well-calibrated according to the calibration curve. The DCA demonstrated that the nomogram was clinically useful.ConclusionThe risk-prediction nomogram for cognitive impairment in people aged over 60 years was effective. All predictors included in this nomogram can be easily accessed from its’ user

Prognostic and therapeutic implications of iron-related cell death pathways in acute myeloid leukemia

Acute myeloid leukemia (AML) is a blood cancer that is diverse in terms of its molecular abnormalities and clinical outcomes. Iron homeostasis and cell death pathways play crucial roles in cancer pathogenesis, including AML. The objective of this study was to examine the clinical significance of genes involved in iron-related cell death and apoptotic pathways in AML, with the intention of providing insights that could have prognostic implications and facilitate the development of targeted therapeutic interventions. Gene expression profiles, clinical information, and molecular alterations were integrated from multiple datasets, including TCGA-LAML and GSE71014. Our analysis identified specific molecular subtypes of acute myeloid leukemia (AML) displaying varying outcomes, patterns of immune cell infiltration, and profiles of drug sensitivity for targeted therapies based on the expression of genes involved in iron-related apoptotic and cell death pathways. We further developed a risk model based on four genes, which demonstrated promising prognostic value in both the training and validation cohorts, indicating the potential of this model for clinical decision-making and risk stratification in AML. Subsequently, Western blot analysis showed that the expression levels of C-Myc and CyclinD1 were significantly reduced after CD4 expression levels were knocked down. The findings underscore the potential of iron-related cell death pathways as prognostic biomarkers and therapeutic targets in AML, paving the way for further research aimed at understanding the molecular mechanisms underlying the correlation between iron balance, apoptosis regulation, and immune modulation in the bone marrow microenvironment

Chemical Composition, Antimicrobial and Insecticidal Activities of Essential Oils of Discarded Perfume Lemon and Leaves (Citrus Limon (L.) Burm. F.) as Possible Sources of Functional Botanical Agents

Two essential oils were isolated from discarded perfume lemon and leaves (Citrus limon (L.) Burm. F.) by hydro-distillation with good yield (0.044% for perfume lemon and 0.338% for leaves). Their biological activities were evaluated against five selected bacterial strains and Aedes albopictus (Ae. albopictus, Diptera: Culicidae). Chemical composition indicated that both essential oils were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpene. These constituents revealed some variability among the oils displaying interesting chemotypes (R)-(+)-limonene (12.29–49.63%), citronellal (5.37–78.70%) and citronellol (2.98–7.18%). The biological assessments proved that the two essential oils had similar effect against bacterial (inhibition zones diameter ranging from 7.27 ± 0.06 to 10.37 ± 0.15 mm; MICs and MBCs ranging from 1.6 to 6.4 mg/mL); against Ae. albopictus larvae (LC(50) ranging from 384.81 to 395.09 ppm) and adult mosquito (LD(50) ranging from 133.059 to 218.962 μg/cm(2)); the activity of the two chemotypes ((R)-(+)-limonene and citronellal): larvae (LC(50) ranging from 267.08 to 295.28 ppm), which were all presented in dose-dependent manners. Through this work, we have showcased that recycling and reusing of agriculture by-products, such as discarded perfume lemon and leaves can produce eco-friendly alternatives in bacterial disinfectants and mosquito control product

Synthesis and biological evaluation of pentacyclic triterpenoid derivatives as potential novel antibacterial agents

A series of ursolic acid (UA), oleanolic acid (OA) and 18β-glycyrrhetinic acid (GA) derivatives were synthesized by introducing a range of substituted aromatic side-chains at the C-2 position after the hydroxyl group at C-3 position was oxidized. Their antibacterial activities were evaluated in vitro against a panel of four Staphylococcus strains. The results revealed that the introduction of aromatic side-chains at the C-2 position of GA led to the discovery of potent triterpenoid derivatives for inhibition of both drug sensitive and resistant S. aureus, while the other two series derivatives of UA and OA showed no significant antibacterial activity even at high concentrations. In particular, GA derivative showed good potency against all four strains of Staphylococcus (MIC = 1.25 - 5 μmol/L) with acceptable pharmacokinetics properties and low cytotoxicity in vitro. Molecular docking was also performed using S. aureus DNA gyrase structure to rationalize the observed antibacterial activity. Therefore, this series of GA derivatives have strong potential for the development of a new type of triterpenoid antibacterial agent

Design of Tunable Terahertz Metamaterial Sensor with Single- and Dual-Resonance Characteristic

We present two types of refractive index sensors by using tunable terahertz (THz) metamaterial (TTM) based on two concentric split-ring resonators (SRRs) with different splits. By modifying the distance between SRRs and substrate, TTM shows tunable single- and dual-resonance characteristic. The maximum tuning range of resonance is 0.432 THz from 0.958 THz to 1.390 THz. To demonstrate a great flexibility of TTM in real application, TTM device is exposed on the surrounding ambient with different refractive index (n). The sensitivity of TTM can be enhanced by increasing SRR height, which is increased from 0.18 THz/RIU to 1.12 THz/RIU under the condition of n = 1.1. These results provide a strategy to improve the sensing performance of the metamaterial-based sensing device by properly arranging the geometric position of meta-atoms. The proposed TTM device can be used for tunable filters, frequency-selective detectors, and tunable high-efficiency sensors in the THz frequency range

Along-Strike Reservoir Development of Steep-Slope Depositional Systems: Case Study from Liushagang Formation in the Weixinan Sag, Beibuwan Basin, South China Sea

Seismic, core, drilling, logging, and thin-section data are considered to analyze the reservoir diversity in the east, middle, and west fan of the Liushagang Formation in the steep-slope zone of the Weixinan Sag, Beibuwan Basin. Three factors primarily affect the reservoir differences for steep-slope systems: (1) Sedimentary factors mostly control reservoir scales and characteristics and the drainage system and microfacies. Massive high-quality reservoirs have shallow burial depths. Channel development and sediment supply favor the formation of these reservoirs. The sedimentary microfacies suggest fan delta plain distributary channels. (2) Lithofacies factors primarily control reservoir types and evolution. The diagenesis of high-quality reservoirs is weak, and a weak compaction–cementation diagenetic facies and medium compaction–dissolution diagenetic facies were developed. (3) Sandstone thickness factors primarily control the oil-bearing properties of reservoirs. The average porosity and permeability of high-quality reservoirs are large, the critical sandstone thickness is small, the average sandstone thickness is large, and the oil-bearing capacity is high. Furthermore, the reservoir prediction models are summarized as fan delta and nearshore subaqueous fan models. The high-quality reservoir of the fan delta model is in the fan delta plain, and the lithology is medium–coarse sandstone. The organic acid + meteoric freshwater two-stage dissolution is developed, various dissolved pores are formed, and a Type I reservoir is developed. The high-quality reservoir of the nearshore subaqueous fan model is in the middle fan, and the lithology is primarily medium–fine sandstone. Only organic acid dissolution, dissolution pores, and Type I–II reservoirs are developed. Regarding reservoir differences and models, the high-quality reservoir of the steep-slope system is shallow and large-scale, and the reservoir is a fan delta plain distributary channel microfacies. Weak diagenetic evolution, good physical properties, thick sandstone, and good oil-bearing properties developed a Type I reservoir. The study of reservoir control factors of the northern steep-slope zone was undertaken in order to guide high-quality reservoir predictions. Further, it provides a reference for high-quality reservoir distribution and a prediction model for the steep-slope system

Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research

Musculoskeletal disorders, including fractures, scoliosis, heterotopic ossification, osteoporosis, osteoarthritis, disc degeneration, and muscular injury, etc., can occur at any stage of human life. Understanding the occurrence and development mechanism of musculoskeletal disorders, as well as the changes in tissues and cells during therapy, might help us find targeted treatment methods. Single-cell techniques provide excellent tools for studying alterations at the cellular level of disorders. However, the application of these techniques in research on musculoskeletal disorders is still limited. This review summarizes the current single-cell and spatial omics used in musculoskeletal disorders. Cell isolation, experimental methods, and feasible experimental designs for single-cell studies of musculoskeletal system diseases have been reviewed based on tissue characteristics. Then, the paper summarizes the latest findings of single-cell studies in musculoskeletal disorders from three aspects: bone and ossification, joint, and muscle and tendon disorders. Recent discoveries about the cell populations involved in these diseases are highlighted. Furthermore, the therapeutic responses of musculoskeletal disorders, especially single-cell changes after the treatments of implants, stem cell therapies, and drugs are described. Finally, the application potential and future development directions of single-cell and spatial omics in research on musculoskeletal diseases are discussed

EFFECT OF miR-663b ON INTERLEUKIN-1β-INDUCED INFLAMMATORY RESPONSE AND APOPTOSIS OF NUCLEUS PULPOSUS CELLS AND ITS MECHANISM

Objective To investigate the effect of miR-663b on the inflammatory response and apoptosis of nucleus pulposus cells (NPCs) induced by interleukin-1β (IL-1β) and its mechanism. Methods According to the different treatment me-thods, NPCs were divided into group A (no treatment), group B (induced by IL-1β), group C (IL-1β induction+miR-663b mimic transfection), and group D (IL-1β induction+miR-633b NC transfection). RT-qPCR was used to measure the expression levels of miR-663b, inflammatory factors [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β], type Ⅱ collagen, and po-lysaccharide in NPCs; CCK-8 assay and TUNEL staining were used to observe the proliferation and apoptosis of NPCs; Western blotting was used to measure the protein expression level of interleukin-1 receptor 1 (IL1R1) in NPCs. TargetScan database was used to predict the potential binding sites between miR-663b and IL1R1. The 293T cells were divided into group E (transfected with IL1R1-wt plasmid+miR-663b mimic), group F (transfected with IL1R1-wt plasmid+miR-663b mimic), group G (transfected with IL1R1-mut plasmid+miR-663b mimic), and group H (transfected with IL1R1-mut plasmid+mimic NC), and the luciferase activity of NPCs was measured for each group. Results RT-qPCR results showed that compared with groups A, B, and D, group C had a significant increase in the relative expression level of miR-663b (t=9.41-22.93,P<0.01), and compared with groups B and D, group C had significant changes in the relative expression levels of TNF-α, IL-6, IL-1β, type Ⅱ collagen, and polysaccharide (t=3.17-32.51,P<0.01). CCK-8 assay and TUNEL staining showed that compared with groups B and D, group C had a significant increase in the proliferation of NPCs (t=3.14,3.96,P<0.01) and a significant reduction in the apoptosis of NPCs (t=4.28,168.61,P<0.01). RT-qPCR and Western blotting showed that compared with groups B and D, group C had signi-ficant reductions in the relative protein expression levels of IL1R1 and IL1R1 in NPCs (t=6.39-12.84,P<0.01). Dual-luciferase reporter assay showed that group E had a significantly lower luciferase activity than groups F, G, and H (t=10.62-16.27,P<0.01). Conclusion This study shows that miR-663b may downregulate the expression of IL1R1 in NPCs through targeted bin-ding to IL1R1 and slow down the inflammatory response and apoptosis of NPCs

Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research

Musculoskeletal disorders, including fractures, scoliosis, heterotopic ossification, osteoporosis, osteoarthritis, disc degeneration, and muscular injury, etc., can occur at any stage of human life. Understanding the occurrence and development mechanism of musculoskeletal disorders, as well as the changes in tissues and cells during therapy, might help us find targeted treatment methods. Single-cell techniques provide excellent tools for studying alterations at the cellular level of disorders. However, the application of these techniques in research on musculoskeletal disorders is still limited. This review summarizes the current single-cell and spatial omics used in musculoskeletal disorders. Cell isolation, experimental methods, and feasible experimental designs for single-cell studies of musculoskeletal system diseases have been reviewed based on tissue characteristics. Then, the paper summarizes the latest findings of single-cell studies in musculoskeletal disorders from three aspects: bone and ossification, joint, and muscle and tendon disorders. Recent discoveries about the cell populations involved in these diseases are highlighted. Furthermore, the therapeutic responses of musculoskeletal disorders, especially single-cell changes after the treatments of implants, stem cell therapies, and drugs are described. Finally, the application potential and future development directions of single-cell and spatial omics in research on musculoskeletal diseases are discussed