Molecular cloning and biochemical characterization of a novel erythrose reductase from Candida magnoliae JH110

<p>Abstract</p> <p>Background</p> <p>Erythrose reductase (ER) catalyzes the final step of erythritol production, which is reducing erythrose to erythritol using NAD(P)H as a cofactor. ER has gained interest because of its importance in the production of erythritol, which has extremely low digestibility and approved safety for diabetics. Although ERs were purified and characterized from microbial sources, the entire primary structure and the corresponding DNA for ER still remain unknown in most of erythritol-producing yeasts. <it>Candida magnoliae </it>JH110 isolated from honeycombs produces a significant amount of erythritol, suggesting the presence of erythrose metabolizing enzymes. Here we provide the genetic sequence and functional characteristics of a novel NADPH-dependent ER from <it>C. magnoliae </it>JH110.</p> <p>Results</p> <p>The gene encoding a novel ER was isolated from an osmophilic yeast <it>C. magnoliae </it>JH110. The ER gene composed of 849 nucleotides encodes a polypeptide with a calculated molecular mass of 31.4 kDa. The deduced amino acid sequence of ER showed a high degree of similarity to other members of the aldo-keto reductase superfamily including three ER isozymes from <it>Trichosporonoides megachiliensis </it>SNG-42. The intact coding region of ER from <it>C. magnoliae </it>JH110 was cloned, functionally expressed in <it>Escherichia coli </it>using a combined approach of gene fusion and molecular chaperone co-expression, and subsequently purified to homogeneity. The enzyme displayed a temperature and pH optimum at 42°C and 5.5, respectively. Among various aldoses, the <it>C. magnoliae </it>JH110 ER showed high specific activity for reduction of erythrose to the corresponding alcohol, erythritol. To explore the molecular basis of the catalysis of erythrose reduction with NADPH, homology structural modeling was performed. The result suggested that NADPH binding partners are completely conserved in the <it>C. magnoliae </it>JH110 ER. Furthermore, NADPH interacts with the side chains Lys252, Thr255, and Arg258, which could account for the enzyme's absolute requirement of NADPH over NADH.</p> <p>Conclusions</p> <p>A novel ER enzyme and its corresponding gene were isolated from <it>C. magnoliae </it>JH110. The <it>C. magnoliae </it>JH110 ER with high activity and catalytic efficiency would be very useful for <it>in vitro </it>erythritol production and could be applied for the production of erythritol in other microorganisms, which do not produce erythritol.</p

Effects of 12 Weeks Weight Training and Plyometric Training on Body Composition, Physical Fitness and Electronic Hogu Hitting Ability in Taekwondo Sparring Athletes

OBJECTIVES The purpose of this study is to identify the effects of differences in muscle function training of Taekwondo sparring athletes on body composition, basic physical fitness, isokinetic muscle function, and electronic hogu hitting ability, and to present basic data for a training program for Taekwondo sparring athletes. METHODS This study randomly sampled 25(M: 20, F: 5) Taekwondo sparring athletes. The sampled subjects were divided into a weight training group (n=8), a plyometric training(plyometric) group (n=8), and a control group (n=9) and trained for 60 minutes, 5 times a week, for 12 weeks. Body composition, basic physical fitness, isokinetic muscle function, and electronic hogu hitting ability were evaluated before and after training. Statistical tests of RM Two-way ANOVA were conducted to verify the interaction between groups and times, main effects of times, and main effects between groups according to 12 weeks of training. Post-hoc was conducted using paired-T test(times) and One-way ANOVA test(groups). RESULTS Taekwondo sparring athletes showed positive changes in body composition(weight, BMI, Lean body mass, % body fat, WHR), basic physical fitness(muscle endurance, flexibility), isokinetic muscle function(knee endurance, low back strength), and electronic hogu hitting ability(round house kick, Turning back kick, number of hit) after participating in weight training for 12 weeks (All p<.05). Additionally, positive changes were observed in flexibility and electronic hogu hitting ability(Turning back kick) after participating in plyometric training for 12 weeks (All p<.05). CONCLUSIONS Weight training for 12 weeks in Taekwondo sparring athletes results in positive changes in body composition, increased flexibility and muscular endurance, increases in knee isokinetic muscular endurance and low back isokinetic strength, and improvement in overall electronic hogu hitting ability. Plyometrics for 12 weeks result in increased flexibility and increased electronic hogu hitting ability for back kick. Weight training shows greater improvement in strength and kick endurance than plyometrics

Using Technology to Facilitate Modeling-Based Science Education: Lessons Learned from a Meta-analysis of Empirical Research

This study focused on the integration of technologies in regular science teaching within the pedagogical framework of modeling-based instruction (MBI), a well-established instructional method in science education, and aimed to identify new trends of technology integration in MBI, explore the particular features (Interactivity, Collaboration, and Scaffolding) and affordances of new technologies, and examine the effect of technology-supported MBI on students learning outcomes. By analyzing empirical MBI studies from 2000 to 2010 through a meta-analysis and qualitatively reviewing studies from 2011-2016, this study shared three major findings: (1) computer-based software was the most commonly used technology in MBI, with Internet and mobile technologies rarely used, thus indicating an alarming gap between technology advancement and its integration in education; (2) the majority of technologies used in MBI were considered highly-interactive, but collaborative and scaffolding features of MBI technologies were rarely discussed in MBI literature; (3) technology-supported MBI had an overall much higher effect size on students’ science learning performance. Implications and suggestions for future research were also discussed

IT Innovation in China: Industry and Business Capabilities

China is investing heavily in leading edge and innovative information technologies to support its continuing economic growth. At the same time, Chinese businesses increasingly need to innovate with IT to create competitive difference and expand internationally. However, to what extent has China developed the capabilities to develop IT innovations and adopt them to generate value and competitive difference? This panel session will build learning on the capabilities both of the software industry and of businesses in China around IT innovation. To create a rich picture of maturity, skills and management processes concerning innovation, this multi-disciplinary session will bring together diverse perspectives from research and practice. By drawing on experience from Europe and the USA, as well as China itself, it will also reflect on key differences and lessons which can be learnt from China’s vast IT investment and rapid acquisition of skills

Optic neuritis associated with seronegative autoimmune encephalitis: a case report

Optic neuritis is an inflammatory demyelinating disorder that primarily affects the optic nerve and is often associated with multiple sclerosis. While it is rare for optic neuritis to be accompanied by autoimmune encephalitis, it can occur in some cases. A 65-year-old woman with bipolar disorder presented with a progressively altered mentality. Magnetic resonance imaging of the brain showed no definite abnormal findings. Electroencephalography revealed nonconvulsive status epilepticus. Cerebrospinal fluid study and autoimmune and paraneoplastic encephalitis antibodies were negative. The patient was diagnosed with seronegative autoimmune encephalitis and treated with methylprednisolone, intravenous immunoglobulin, and rituximab. Her condition gradually improved except for persistent blindness on the left side. This case highlights the importance of considering autoimmune encephalitis even in the absence of identifiable pathogenic antibodies when clinical manifestations and response to immunotherapy support such a diagnosis

IT Innovation in China: Industry and Business Capabilities

China is investing heavily in leading-edge and innovative information technologies to support its continuing economic growth. At the same time, Chinese businesses increasingly need to innovate with IT to create competitive difference and expand internationally. However, to what extent has China developed the capabilities to develop IT innovations and adopt them successfully? This 2011 ICIS panel session built a rich picture of maturity, skills, and management processes concerning IT adoption and innovation, bringing together diverse perspectives from research and practice. By drawing on experience from Europe and the U.S., as well as China itself, it also reflected on distinct aspects of the Chinese business environment and the impact of these on IT

Observation of interlayer phonon modes in van der Waals heterostructures

We have investigated the vibrational properties of van der Waals heterostructures of monolayer transition metal dichalcogenides (TMDs), specifically MoS2/WSe2 and MoSe2/MoS2 heterobilayers as well as twisted MoS2 bilayers, by means of ultralow-frequency Raman spectroscopy. We discovered Raman features (at 30 ~ 40 cm-1) that arise from the layer-breathing mode (LBM) vibrations between the two incommensurate TMD monolayers in these structures. The LBM Raman intensity correlates strongly with the suppression of photoluminescence that arises from interlayer charge transfer. The LBM is generated only in bilayer areas with direct layer-layer contact and atomically clean interface. Its frequency also evolves systematically with the relative orientation between of the two layers. Our research demonstrates that LBM can serve as a sensitive probe to the interface environment and interlayer interactions in van der Waals materials

Passivity, task segmentation, and relational capital: a study of interpersonal relationship formation among freshman medical students in team-based activities

Purpose Team-based learning in medical education facilitates the formation of interpersonal relationships, enhances academic achievement, and establishes future clinical connections. Despite medical schools’ distinct educational environments and curricula, research investigating students’ learning and behavioral characteristics within this context remains limited. This study aimed to investigate the specific characteristics of interpersonal relationship formation among freshmen medical students in team-based learning activities. Methods Focus group interviews were conducted with 11 students who participated in a team-based class held during the first semester of the premedical year. Results Medical students passively expressed their thoughts and feelings, such as active sympathy for the team’s opinions and self-censorship of SNS (social networking service) conversations. When performing team activities, students were excessively conscious of others and focused on evenly dividing their work, minimizing their opinions, and fulfilling their share to prevent potential interpersonal conflicts. Interpersonal activities were only superficial, as students intentionally maintained a certain distance from team members or used team activities as an opportunity to accumulate relational capital. Conclusion The results of this study are expected to provide a useful basis for designing and conducting team activity classes to enhance interpersonal relationship formation