The role of cytosolic glutamine synthetases in abiotic stress and development in Arabidopsis thaliana

Glutamine (Gln), a major nitrogen source in plants, is considered a central intermediate that coordinates carbon-nitrogen assembly for plant growth and development. To maintain a sufficient Gln supply, plant cells employ glutamine synthetases (GS), including cytosolic GS1 and plastidic GS2 for Gln production. Previous work has shown that the GS1 is responsive to various environmental stresses. This study demonstrated the involvement of GS1s in Gln homeostasis and the role of GS1 in abiotic stress tolerance in Arabidopsis. The GS1 family is comprised of five isoforms in Arabidopsis thaliana. Gene expression profiling showed that GLN1;1, GLN1;3 and GLN1;4 had similar expression patterns and were upregulated by abiotic (salinity and cold) stresses, whereas GLN1;2 exhibited constitutive expression and no GLN1;5 transcript was detected under any of the conditions tested. Null T-DNA insertion mutants for the five GS1 genes were obtained. Only the gln1;1 mutant displayed enhanced sensitivity to a GS inhibitor, phosphinothricin, and to cold and salinity treatments, suggesting a nonredundant role for GLN1;1. Increased stress sensitivity in gln1;1 was associated with accelerated accumulation of reactive oxygen species (ROS), particularly in chloroplasts. To better understand the role of cytosolic GS isoforms, we generated two different triple mutant combinations. Triple mutant gln1;1/gln1;2/gln1;3 showed reduced growth at an early stage. The gln1;1/gln1;3/gln1;4 mutant is pollen lethal, indicating an essential role of Gln in plant gametophyte development. Collectively, our results establish a link between cytosolic Gln production, ROS accumulation, plant stress tolerance and development

Numerical Investigation of Flow and Heat Transfer Characteristics in Plate with Multiple Incline Stage Holes

In this paper, the effects of impingement and film composite cooling on the design of combustion chamber cooling structure are simulated by numerical simulation. The focus of the investigation was on increased film cooling efficiency and enhanced heat transfer between the coolant and the hole wall. The five-stage shaped hole model and one cylindrical hole design have the same equivalent flow area. The flow and heat transfer characteristics of cylindrical hole and stage-shaped hole were numerically investigated under same blowing ratio, and compared at the same blowing ratio. The results showed the stage-shaped hole resulted in higher cooling effectiveness, especially in rear part, and the mechanisms of which were studied in details. The consequences of the phase parameters in the flow have very clearly dependedt on the internal shape of the corresponding hole. Stage-shaped holes formed impact inside the wall, tapped the coolant potential in cooling, and increased the heat transfer inside the solid wall. Further, stage-shaped hole resulted in unstabilized flow inside hole, gave an enhancement of lateral spreading ability, and brought a significant increase of the film lateral effectiveness. Further, the affection of area ratio and height ratio has been studied by five models. The results show that the increasing of area ratio leads to an increase in cooling efficiency, which also indicates the increasing of height ratio showed slight affection

Entanglement Routing over Quantum Networks Using Greenberger-Horne-Zeilinger Measurements

Generating a long-distance quantum entanglement is one of the most essential functions of a quantum network to support quantum communication and computing applications. The successful entanglement rate during a probabilistic entanglement process decreases dramatically with distance, and swapping is a widely-applied quantum technique to address this issue. Most existing entanglement routing protocols use a classic entanglement-swapping method based on Bell State measurements that can only fuse two successful entanglement links. This paper appeals to a more general and efficient swapping method, namely n-fusion based on Greenberger-Horne-Zeilinger measurements that can fuse n successful entanglement links, to maximize the entanglement rate for multiple quantum-user pairs over a quantum network. We propose efficient entanglement routing algorithms that utilize the properties of n-fusion for quantum networks with general topologies. Evaluation results highlight that our proposed algorithm under n-fusion can greatly improve the network performance compared with existing ones

Efficient subspace skyline query based on user preference using MapReduce

Subspace skyline, as an important variant of skyline, has been widely applied for multiple-criteria decisions, business planning. With the development of mobile internet, subspace skyline query in mobile distributed environments has recently attracted considerable attention. However, efficiently obtaining the meaningful subset of skyline points in any subspace remains a challenging task in the current mobile internet. For more and more mobile applications, subspace skyline query on mobile units is usually limited by big data and wireless bandwidth. To address this issue, in this paper, we propose a system model that can support subspace skyline query in mobile distributed environment. An efficient algorithm for processing the Subspace Skyline Query using MapReduce (SSQ) is also presented which can obtain the meaningful subset of points from the full set of skyline points in any subspace. The SSQ algorithm divides a subspace skyline query into two processing phases: the preprocess phase and the query phase. The preprocess phase includes the pruning process and constructing index process which is designed to reduce network delay and response time. Additionally, the query phase provides two filtering methods, SQM-filtering and ε-filtering, to filter the skyline points according to user preference and reduce network cost. Extensive experiments on real and synthetic data are conducted and the experimental results indicate that our algorithm is much efficient, meanwhile, the pruning strategy can further improve the efficiency of the algorithm

Experimental investigation of flow and heat transfer characteristics on matrix ribbed channel

The effect of the rib width to height ratio t/e and width to pitch ratio t/p on the local heat transfer distribution in a rectangular matrix ribbed channel with two opposite in line o 45 ribs are experimentally investigated for Reynolds Numbers from 54000 to 150000. The rib height to channel height ratio e/H is 0.5, t/p and t/e both varies in range of 0.3-0.5. And to simulate the actually situation in turbine blades, and provide useful direct results for turbine blade designers, the parameters are same with the blade. The experiments results show that, in comparison to fully developed flow in a smooth pipe of equivalent hydraulic diameter, the Nusselt number inside the matrix-ribbed rectangular channel is increased up to 5 to 9 times higher, while total pressure drop is enlarged by up to significant magnitude. The Nusselt number ratio increases with t/p and t/e increased. Semi-empirical heat transfer is developed for designing of cooling channel

Research in nonlinearity of surface acoustic wave devices

Surface acoustic wave (SAW) devices are one of the indispensable components in the radio frequency (RF) front-end of mobile phones. With the development of mobile communication technology, the requirements for linear specification of devices are more and more strict. Nonlinear distortions of SAW devices have a serious influence on the application of mobile RF modules. To satisfy the strict requirement of linearity of communication system, it is necessary to understand the generation mechanism of nonlinearity and study the accurate modeling, appropriate measurement methods, and nonlinear response elimination technology. In this paper, we summarize the research progress on the nonlinearity of SAW devices in recent years from four aspects: the generation mechanism, simulation methods, measurement system, and suppression technology. The nonlinear harmonics with the nonlinear Mason equivalent circuit model are simulated. Furthermore, harmonics and intermodulation signals of SAW filters are tested by the authors. Thanks to these research studies, it is of great significance to the development of future RF front-end modules with high linear SAW devices

SP1-Induced Upregulation of lncRNA LINC00659 Promotes Tumour Progression in Gastric Cancer by Regulating miR-370/AQP3 Axis

Growing evidence demonstrates that long noncoding RNAs (lncRNAs) play critical roles in various human tumors. LncRNA LINC00659 (LINC00659) is a newly identified lncRNA and its roles in tumors remain largely unclear. In this study, we elucidated the potential functions and molecular mechanisms of LINC00659 on the biological behaviors of gastric cancer (GC), and also explored its clinical significance. We firstly demonstrated that LINC00659 levels were distinctly up-regulated in both GC specimens and cells using bioinformatics analysis and RT-PCR. The results of ChIP assays and luciferase reporter assays confirmed that upregulation of LINC00659 was activated by SP1 in GC. Clinical assays revealed that higher levels of LINC00659 were associated with TNM stage, lymphatic metastasis, and poorer prognosis. Moreover, LINC00659 was confirmed to be an independent prognostic marker for the patients with GC using multivariate assays. Lost-of-function assays indicated that knockdown of LINC00659 suppressed the proliferation, metastasis, and EMT progress of GC cells in vitro. Mechanistic investigation indicated that LINC00659 served as a competing endogenous RNA (ceRNA) for miR-370, thereby resulting in the upregulation of leading to the depression of its endogenous target gene AQP3. Overall, our present study revealed that the LINC00659/miR-370/AQP3 axis contributes to GC progression, which may provide clues for the exploration of cancer biomarkers and therapeutic targets for GC