Effects of Short and Medium Term use of Artesunate on Fertility in Male Rats

The effects of artesunate on major sperm parameters and testicular history in wistar rats were examined. Sixteen sexually mature wistar rats weighing 150-180gm were divided into 4 groups and administered 2mg/kg or 1mg/kg of the drug for period ranging 2-8weeks. Control animals were administered water only. All animals were allowed food and water ad-libitum. At the end of these periods, the animals were euthanized and their cauda epididymes, testes, prostrate and seminal vesicles were neatly removed from them. Major sperm parameters were obtained from the epididymal sperm and the testis prepared and examined histologically. Results showed normal to raise sperm parameters in both groups treated for 2 weeks. Sperm density in millions/gm of testes in groups 2 and 3 which received different doses of the drug for 2 weeks was 62.0+10.2 and 65.6+13.8 respectively compared to 52.1+11.0 in control rats. However after 4 weeks on the drug all major parameters were significantly depressed. Sperm density was 20.3+1.5 and abnormal sperm rate 71.7% compared to 52.1+11.0 and 26.7+2.3% in control animals respectively. This study suggests that under certain conditions, artesunate will suppress spermatogenesis. Until exhaustive studies of the drug\'s effects on fertility are accomplished, it must be used with appropriate caution in young people. Keywords: Artesunate, spermatogenesis, sperm parameters. Nigerian Journal of Health and Biomedical Sciences Vol. 7 (2) 2008: pp. 18-2

Nutritional and haematological parameters of cleft lip and/or palate patients in Lagos, Nigeria.

Introduction: Cleft Lip and Palate (CLP) deformities are the most common congenital abnormalities of the orofacial structures. The deformity is associated with certain peculiar problems including feeding disorders and recurrent infections. These may combine to affect the nutritional status of the patients. This study was carried out to assess the prevalence of malnutrition among patients with CLP deformities in Lagos, review the prevalence of anaemia and the burden of bacterial infections.Materials/methods: Serum albumin levels of patients with CLP invited for surgical intervention in Lagos between 2006 and 2008 were assessed to determine the nutritional status, the Packed Cell Volume for the presence of anaemia and total White Cell Count (WBC) to screen for the possibility of bacterial infection.Results: Two hundred and twenty five patients were studied comprising of 112 males and 113 females. Mean age was 5.05 ± 7.73 years. One hundred and one (44.89%) patients had complete cleft of the lip and palate, 27.22% had cleft lip, 13.78% had cleft of the lip and hard palate, while 7.11% had cleft of the soft palate alone. Various types of rare cleft deformities occurred in 8.88% patients out of which macrostomia or lateral cleft constituted 6.67%. Forty-eight (21.3%)patients had malnutrition. The type of CLP deformities was found to have no significant effect on the serum albumin level (p = 0. 089). Sixty-eight (30.2%) patients had anaemia. There was an association between the types of CLP deformities and the PCV levels (P=0.001). Twenty-two (9.8%) patients showed haematologic evidences of on-going bacterial infections with raised total WBC count.Conclusion: The prevalence of anaemia in patients with CLP is however higher than that of the general population. Patients with CLP require nutrition support and continuous medical monitoring

Repeated Stress Exaggerates Lipopolysaccharide-Induced Inflammatory Response in the Rat Spleen

Spleen is an immune organ innervated with sympathetic nerves which together with adrenomedullary system control splenic immune functions. However, the mechanism by which prior stress exposure modulates the immune response induced by immunogenic challenge is not sufficiently clarified. Thus, the aim of this study was to investigate the effect of a single (2 h) and repeated (2 h daily for 7 days) immobilization stress (IMO) on the innate immune response in the spleen induced by lipopolysaccharide (LPS, 100 microg/kg). LPS elevated splenic levels of norepinephrine and epinephrine, while prior IMO prevented this response. LPS did not alter de novo production of catecholamines, however, prior IMO attenuated phenylethanolamine N-methyltransferase gene expression. Particularly repeated IMO exacerbated LPS-induced down-regulation of alpha1B- and beta1-adrenergic receptors (ARs), while enhanced alpha2A- and beta2-AR mRNAs. Elevated expression of inflammatory mediators (iNOS2, IL-1beta, IL-6, TNF-alpha, IL-10) was observed following LPS and repeated IMO again potentiated this effect. These changes were associated with enhanced Ly6C gene expression, a monocyte marker, and elevated MCP-1, GM-CSF, and CXCL1 mRNAs suggesting an increased recruitment of monocytes and neutrophils into the spleen. Additionally, we observed increased Bax/Bcl-1 mRNA ratio together with reduced B cell numbers in rats exposed to repeated IMO and treated with LPS but not in acutely stressed rats. Altogether, these data indicate that repeated stress via changes in CA levels and specific alpha- and beta-AR subtypes exaggerates the inflammatory response likely by recruiting peripheral monocytes and neutrophils to the spleen, resulting in the induction of apoptosis within this tissue, particularly in B cells. These changes may alter the splenic immune functions with potentially pathological consequences