Increased traffic safety by means of intelligent detection and localization technologies

The two technologies presented in this paper, "Communication Location Surveillance" (CLS) and radar classification by means of evaluation of micro-Doppler spectra, have been adapted and further developed for use in critical and complex traffic situations. The aim of the joint research activity of DLR and IMST is to detect potentially dangerous traffic situations at an early stage and, in particular, to protect vulnerable road users who do not usually communicate digitally with other road users. This is done by precisely locating road users and recording their direction of movement and speed. On the other hand, it must be possible to classify the participants in order to be able to assess their particular potential danger. Only with an exact situation overview of a traffic situation can predictions be made to assess the danger and possible countermeasures be initiated. This includes, for example, warnings to the participants via acoustic or optical signals, via digital vehicle communication or active intervention in a traffic light control system

The Ccr4–Not Deadenylase Subunits CNOT7 and CNOT8 Have Overlapping Roles and Modulate Cell Proliferation

Accurate gene expression requires the precise control of mRNA levels, which are determined by the relative rates of nuclear (pre-)mRNA synthesis and processing, and cytoplasmic mRNA turnover. A key step in mRNA degradation is the removal of the poly(A) tail, which involves several deadenylases including components of the Ccr4–Not complex. Here, we focused on the role of the human paralogues CNOT7 (hCaf1/Caf1a) and CNOT8 (hPop2/Caf1b/Calif), which possess deadenylase activity mediated by DEDD nuclease domains. We show that efficient proliferation requires both subunits, although combined knockdown of CNOT7 and CNOT8 further reduces cell proliferation indicating partial redundancy between these proteins. Interestingly, the function of CNOT7 in cell proliferation partly depends on its catalytic activity. On the other hand, the interaction between CNOT7 and BTG2, a member of the antiproliferative BTG/Tob family involved in transcription and mRNA decay appears less important for proliferation of MCF7 cells, suggesting that CNOT7 does not function solely in conjunction with BTG2. Further analysis of gene expression profiles of CNOT7 and/or CNOT8 knockdown cells underscores the partial redundancy between these subunits and suggests that regulation of several genes, including repression of the antiproliferative genes MSMB and PMP22, by the Ccr4–Not complex contributes to cell proliferation