Nylon membrane as a fluorimetric probe for the herbicide bentazone

The fluorimetric signal produced by bentazone retained in selected solid surfaces was investigated. Among the different tested supports, only a microporous nylon membrane produced the desired signal. The quantitative study was carried out by second-order calibration using parallel factor analysis, allowing the determination in a highly interfering medium. A detection limit of 0.4 ng mL−1, a prediction relative error of 8%, and a sample frequency of ten samples per hour were obtained in spiked natural waters using green analytical chemistry principles.Fil: Chiarandini Fiore, Jessica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Escandar, Graciela Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentin

Diallyl Disulfide prevention of Cis-diammine Dichloroplatinum–induced Nephrotoxicity and Leukopenia in rats: potential adjuvant effects

Cisplatin (CisPt) is an effective chemotherapeutic agent against several human cancers, but it produces important nephrotoxicity, leukopenia, and mortality. In this work, we report initial results on the potential ability of diallyl disulfide (DADS) to block these toxicities without compromising chemotherapy. Male Sprague Dawley rats were used (control, DADS, CisPt, and CisPt/DADS). CisPt was administered sc as a single dose (10.5 mg/kg) in saline. DADS was given daily intragastrically in olive oil (292.5 mg/kg) 1 h before CisPt administration the first day and 146.25 mg/kg during the next 3 days. The animals were sacrificed at the fifth day after CisPt administration. DADS significantly decreased CisPt-induced nephrotoxicity as evaluated by histology and by seric urea (CisPt: 11.05 ± 3.59; CisPt/DADS: 6.53 ± 1.74) and creatinine (CisPt: 24.74 ± 3.03; CisPt/DADS: 14.83 ± 2.07). DADS also decreased leukopenia (CisPt: 13.5% and CisPt/DADS: 43.4% respect the control), and mortality (CisPt: 50%; CisPt/DADS: 29%). DADS showed ability to interact with reactive oxygen species (H2O2, hydroperoxides, OH•) and with iron. DADS treatment does not change Platinum levels in kidney (CisPt: 15.2 ± 5.1; CisPt/DADS: 13.9 ± 4.5). Because DADS is known to inhibit cellular replication and to promote apoptosis of tumor cells, results suggest that DADS merit to be tested as a potential coadjuvant of CisPt chemotherapy in tumor-bearing animals.Fil: Chiarandini Fiore, Jessica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: de Ferreyra, Elida C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentin

Determination of the molar mass of the humic substances of the Chubut River (Argentina) by electrospray ionization mass spectrometry

Here, we studied the number-average molar mass of the humic substances of the Chubut River (Argentina) in order to progress in their characterization. We used electrospray ionization mass spectrometry in positive mode because of its high accuracy and non-destructive nature. The result obtained (818. Da) is a relatively low value, typical of fulvic acids.Fil: Scapini, Maria del Carmen. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Conzonno, Victor Hugo. Universidad Nacional de la Plata. Facultad de Ciencias Agrarias y Forestales. Laboratorio de Investigacion de Sistemas Ecologicos y Ambientales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Orfila, Jorge D.. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Chiarandini Fiore, Jessica Paola. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Balzaretti, Vilma Teresa. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Trelew; ArgentinaFil: Fernandez Cirelli, Alicia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Estudios Transdisciplinarios del Agua; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Excitation-emission matrices applied to the study of urban effluent discharges in the Chubut River (Patagonia, Argentina)

Natural and contaminated waters of the final reaches of the Chubut River (Patagonia, Argentina) were studied to obtain information about river organic matter and effects of domestic and industrial discharges (fishery effluents and sewages). Fluorescence Excitation–Emission Matrices (EEMs) were obtained from samples only filtered (0.45 μm) and diluted, if necessary, to avoid the inner filter effect. In addition, physicochemical parameters were measured to know the quality of the water and the effluents. Results show that EEMs allow a rapid and simple control of the effluents from fisheries and domestic sewage in Chubut River estuary, necessary to take management decisions.Fil: Chiarandini Fiore, Jessica Paola. Universidad de Rosario. Facultad de Bioquímica y Ciencias Farmacéuticas; Argentina.Fil: Scapini, María del Carmen. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales. Argentina.Fil: Olivieri, Alejandro César. Universidad de Rosario. Facultad de Bioquímica y Ciencias Farmacéuticas; Argentina