Submissive, inhibited, avoidant, and escape motivated: The correlates and consequences of arm-crossing.

Some scholars of nonverbal behavior contend that arm-crossing indicates a defensive orientation to the social environment, but relevant evidence is sparse. Three studies (N = 242) sought to investigate whether there is truth to this idea. Consistent with it, Study 1 found that people reporting higher arm-crossing frequencies scored higher in interpersonal submissiveness and were more inhibited in their social decision-making. To investigate causal processes, Studies 2 and 3 manipulated arm-crossing using a hypothesis-disguising cover story. Study 2 found that arm-crossing activated thoughts of the self’s submissiveness and social vulnerability. Study 3 focused on activated strategies for handling potential interpersonal violence. Participants in an arm-crossing condition, relative to a control condition, indicated that they would be more inclined to escape and less likely to attack. The studies converge on the idea that arm-crossing can signify and cause a defensive social orientation

The scope and consequences of metaphoric thinking: Using individual differences in metaphor usage to understand how metaphor functions.

People often think, feel, and behave metaphorically according to conceptual metaphor theory. There are normative sources of support for this theory, but individual differences have received scant attention. This is surprising because people are likely to differ in the frequency with which they use metaphors and therefore the frequency with which they experience the costs and benefits of metaphoric thinking. To investigate these ideas, a five study program of research (total N = 532) was conducted. Study 1 developed and validated a metaphor usage measure, finding that people were fairly consistent in their tendencies toward literal thought and language on the one hand versus metaphoric thought and language on the other. These differences were, in turn, consequential. Although metaphor usage predicted susceptibility to metaphor transfer effects (Studies 2-3), it was also linked to higher levels of emotional understanding (Studies 4-5). The findings provide support for several key premises of conceptual metaphor theory in the context of a new measure that can be used to track the consequences of metaphoric thinking

WNT5A-JNK regulation of vascular insulin resistance in human obesity

Obesity is associated with the development of vascular insulin resistance; however, pathophysiological mechanisms are poorly understood. We sought to investigate the role of WNT5A-JNK in the regulation of insulin-mediated vasodilator responses in human adipose tissue arterioles prone to endothelial dysfunction. In 43 severely obese (BMI 44±11 kg/m2) and five metabolically normal non-obese (BMI 26±2 kg/m2) subjects, we isolated arterioles from subcutaneous and visceral fat during planned surgeries. Using videomicroscopy, we examined insulin-mediated, endothelium-dependent vasodilator responses and characterized adipose tissue gene and protein expression using real-time polymerase chain reaction and Western blot analyses. Immunofluorescence was used to quantify endothelial nitric oxide synthase (eNOS) phosphorylation. Insulin-mediated vasodilation was markedly impaired in visceral compared to subcutaneous vessels from obese subjects (pWNT5A and its non-canonical receptors, which correlated negatively with insulin signaling. Pharmacological JNK antagonism with SP600125 markedly improved insulin-mediated vasodilation by sixfold (p

Mitochondrial Genetic Background Modulates Bioenergetics and Susceptibility to Acute Cardiac Volume Overload

Dysfunctional bioenergetics has emerged as a key feature in many chronic pathologies such as diabetes and cardiovascular disease. This has led to the mitochondrial paradigm in which it has been proposed that mtDNA sequence variation contributes to disease susceptibility. In the present study we show a novel animal model of mtDNA polymorphisms, the MNX (mitochondrial–nuclear exchange) mouse, in which the mtDNA from the C3H/HeN mouse has been inserted on to the C57/BL6 nuclear background and vice versa to test this concept. Our data show a major contribution of the C57/BL6 mtDNA to the susceptibility to the pathological stress of cardiac volume overload which is independent of the nuclear background. Mitochondria harbouring the C57/BL6J mtDNA generate more ROS (reactive oxygen species) and have a higher mitochondrial membrane potential relative to those with C3H/HeN mtDNA, independent of nuclear background. We propose this is the primary mechanism associated with increased bioenergetic dysfunction in response to volume overload. In summary, these studies support the ‘mitochondrial paradigm’ for the development of disease susceptibility, and show that the mtDNA modulates cellular bioenergetics, mitochondrial ROS generation and susceptibility to cardiac stress

Developmental exposure to second-hand smoke increases adult atherogenesis and alters mitochondrial DNA copy number and deletions in apoE(-/-) mice.

Cardiovascular disease is a major cause of morbidity and mortality in the United States. While many studies have focused upon the effects of adult second-hand smoke exposure on cardiovascular disease development, disease development occurs over decades and is likely influenced by childhood exposure. The impacts of in utero versus neonatal second-hand smoke exposure on adult atherosclerotic disease development are not known. The objective of the current study was to determine the effects of in utero versus neonatal exposure to a low dose (1 mg/m(3) total suspended particulate) of second-hand smoke on adult atherosclerotic lesion development using the apolipoprotein E null mouse model. Consequently, apolipoprotein E null mice were exposed to either filtered air or second-hand smoke: (i) in utero from gestation days 1-19, or (ii) from birth until 3 weeks of age (neonatal). Subsequently, all animals were exposed to filtered air and sacrificed at 12-14 weeks of age. Oil red-O staining of whole aortas, measures of mitochondrial damage, and oxidative stress were performed. Results show that both in utero and neonatal second-hand smoke exposure significantly increased adult atherogenesis in mice compared to filtered air controls. These changes were associated with changes in aconitase and mitochondrial superoxide dismutase activities consistent with increased oxidative stress in the aorta, changes in mitochondrial DNA copy number and deletion levels. These studies show that in utero or neonatal exposure to second-hand smoke significantly influences adult atherosclerotic lesion development and results in significant alterations to the mitochondrion and its genome that may contribute to atherogenesis

The Impact of Influencers on Cigar Promotions: A Content Analysis of Large Cigar and Swisher Sweets Videos on TikTok

Little is known about the content, promotions, and individuals in cigar-related videos on TikTok. TikTok videos with large cigar and Swisher Sweets-related hashtags between July 2016 and September 2020 were analyzed. Follower count was used to identify influencers. We compared content characteristics and demographics of featured individuals between cigar types, and by influencer status. We also examined the association between content characteristics and video engagement. Compared to large cigar videos, Swisher Sweets videos were more likely to feature arts and crafts with cigar packages, cannabis use, and flavored products. In addition, Swisher Sweets videos were also more likely to feature females, Black individuals, and younger individuals. Both Swisher Sweets and large cigar influencers posted more videos of cigar purchasing behaviors than non-influencers, which was associated with more video views. None of the videos disclosed sponsorship with #ad or #sponsored. Videos containing the use of cigar packages for arts and crafts, and flavored products highlight the importance of colorful packaging and flavors in the appeal of Swisher Sweets cigars, lending support for plain packaging requirements and the prohibition of flavors in cigar products to decrease the appeal of cigars. The presence and broad reach of cigar promotions on TikTok requires stricter enforcement of anti-tobacco promotion policies

A bioinformatics pipeline for estimating mitochondrial DNA copy number and heteroplasmy levels from whole genome sequencing data

Mitochondrial diseases are a heterogeneous group of disorders that can be caused by mutations in the nuclear or mitochondrial genome. Mitochondrial DNA (mtDNA) variants may exist in a state of heteroplasmy, where a percentage of DNA molecules harbor a variant, or homoplasmy, where all DNA molecules have the same variant. The relative quantity of mtDNA in a cell, or copy number (mtDNA-CN), is associated with mitochondrial function, human disease, and mortality. To facilitate accurate identification of heteroplasmy and quantify mtDNA-CN, we built a bioinformatics pipeline that takes whole genome sequencing data and outputs mitochondrial variants, and mtDNA-CN. We incorporate variant annotations to facilitate determination of variant significance. Our pipeline yields uniform coverage by remapping to a circularized chrM and by recovering reads falsely mapped to nuclear-encoded mitochondrial sequences. Notably, we construct a consensus chrM sequence for each sample and recall heteroplasmy against the sample's unique mitochondrial genome. We observe an approximately 3-fold increased association with age for heteroplasmic variants in non-homopolymer regions and, are better able to capture genetic variation in the D-loop of chrM compared to existing software. Our bioinformatics pipeline more accurately captures features of mitochondrial genetics than existing pipelines that are important in understanding how mitochondrial dysfunction contributes to disease

Experimental exposure regimen to second-hand smoke (SHS).

<p>apoE−/− mice on a C57BL/6J background were developmentally exposed to either filtered air or 1 mg/m³ total suspended particulate (TSP) SHS. The <i>in utero</i> group were exposed to 1 mg/m³ TSP from gestation days 1–19, followed by filtered air exposure until sacrifice at 12–14 weeks of age. The neonatal group were exposed to 1 mg/m³ TSP within 24 hours of birth until weaning at 3 weeks of age, followed by filtered air exposure until sacrifice at 12–14 weeks of age. Control mice were exposed exclusively to filtered air, until sacrifice at 12–14 weeks of age.</p

Aconitase activity from aortas harvested from mice exposed to SHS during development.

<p>Aconitase is specifically inactivated by superoxide (O₂^.−) and peroxynitrate (ONOO⁻) and therefore provides an indirect measure of O₂^−. associated oxidant stress. A) Aconitase activity was measured in aorta homogenates from mice exposed to filtered air, neonatal SHS, or <i>in utero</i> SHS by following the production of cis-aconitate from isocitrate at 240 nm. An asterisk (*) indicates a significant difference exists compared to filtered air control (p≤0.001; n = 8/group). B) Immunoblot showing aconitase protein levels; the bar graph below depicts the quantification of relative aconitase protein levels to control (n = 8/group).</p